New insights on sucrose metabolism: Evidence for an active A/N-Inv in chloroplasts uncovers a novel component of the intracellular carbon trafficking

The presence of sucrose (Suc) in plastids was questioned for several decades. Although it was reported some decades ago, neither Suc transporters nor Suc metabolizing enzymes were demonstrated to be active in those organelles. By biochemical, immunological, molecular and genetic approaches we show t...

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Detalles Bibliográficos
Autores: Vargas, Walter Alberto, Pontis, Horacio Guillermo, Salerno, Graciela Lidia
Tipo de recurso: artículo
Estado:Versión publicada
Fecha de publicación:2007
País:Argentina
Institución:Consejo Nacional de Investigaciones Científicas y Técnicas
Repositorio:CONICET Digital (CONICET)
Idioma:inglés
OAI Identifier:oai:ri.conicet.gov.ar:11336/131848
Acceso en línea:http://hdl.handle.net/11336/131848
Access Level:acceso abierto
Palabra clave:ALKALINE/NEUTRAL INVERTASE
ARABIDOPSIS
CHLOROPLASTS
SPINACH SUBCELLULAR LOCALIZATION
SUCROSE HYDROLYSIS
https://purl.org/becyt/ford/1.6
https://purl.org/becyt/ford/1
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oai_identifier_str oai:ri.conicet.gov.ar:11336/131848
network_acronym_str AR
network_name_str Argentina
repository_id_str
dc.title.none.fl_str_mv New insights on sucrose metabolism: Evidence for an active A/N-Inv in chloroplasts uncovers a novel component of the intracellular carbon trafficking
title New insights on sucrose metabolism: Evidence for an active A/N-Inv in chloroplasts uncovers a novel component of the intracellular carbon trafficking
spellingShingle New insights on sucrose metabolism: Evidence for an active A/N-Inv in chloroplasts uncovers a novel component of the intracellular carbon trafficking
Vargas, Walter Alberto
ALKALINE/NEUTRAL INVERTASE
ARABIDOPSIS
CHLOROPLASTS
SPINACH SUBCELLULAR LOCALIZATION
SUCROSE HYDROLYSIS
https://purl.org/becyt/ford/1.6
https://purl.org/becyt/ford/1
title_short New insights on sucrose metabolism: Evidence for an active A/N-Inv in chloroplasts uncovers a novel component of the intracellular carbon trafficking
title_full New insights on sucrose metabolism: Evidence for an active A/N-Inv in chloroplasts uncovers a novel component of the intracellular carbon trafficking
title_fullStr New insights on sucrose metabolism: Evidence for an active A/N-Inv in chloroplasts uncovers a novel component of the intracellular carbon trafficking
title_full_unstemmed New insights on sucrose metabolism: Evidence for an active A/N-Inv in chloroplasts uncovers a novel component of the intracellular carbon trafficking
title_sort New insights on sucrose metabolism: Evidence for an active A/N-Inv in chloroplasts uncovers a novel component of the intracellular carbon trafficking
dc.creator.none.fl_str_mv Vargas, Walter Alberto
Pontis, Horacio Guillermo
Salerno, Graciela Lidia
author Vargas, Walter Alberto
author_facet Vargas, Walter Alberto
Pontis, Horacio Guillermo
Salerno, Graciela Lidia
author_role author
author2 Pontis, Horacio Guillermo
Salerno, Graciela Lidia
author2_role author
author
dc.subject.none.fl_str_mv ALKALINE/NEUTRAL INVERTASE
ARABIDOPSIS
CHLOROPLASTS
SPINACH SUBCELLULAR LOCALIZATION
SUCROSE HYDROLYSIS
https://purl.org/becyt/ford/1.6
https://purl.org/becyt/ford/1
topic ALKALINE/NEUTRAL INVERTASE
ARABIDOPSIS
CHLOROPLASTS
SPINACH SUBCELLULAR LOCALIZATION
SUCROSE HYDROLYSIS
https://purl.org/becyt/ford/1.6
https://purl.org/becyt/ford/1
description The presence of sucrose (Suc) in plastids was questioned for several decades. Although it was reported some decades ago, neither Suc transporters nor Suc metabolizing enzymes were demonstrated to be active in those organelles. By biochemical, immunological, molecular and genetic approaches we show that alkaline/neutral invertases (A/N-Invs) are also localized in chloroplasts of spinach and Arabidopsis. A/N-Inv activity and polypeptide content were shown in protein extracts from intact chloroplasts. Moreover, we functionally characterized the Arabidopsis At-A/N-InvE gene coding for a chloroplast-targeted A/N-Inv. The At-A/N-InvE knockout plants displayed a lower total A/N-Inv activity in comparison with wild-type plants. Furthermore, neither A/N-Inv activity nor A/N-Inv polypeptides were detected in protein extracts prepared from chloroplasts of mutant plants. Also, the measurement of carbohydrate content, in leaves harvested either at the end of the day or at the end of the night period, revealed that the knockout plants showed a decrease in starch accumulation but no alteration in Suc levels. These are the first results demonstrating the presence of a functional A/N-Inv inside chloroplasts and its relation with carbon storage in Arabidopsis leaves. Taken together our data and recent reports, we conclude that the participation of A/N-Invs in the carbon flux between the cytosol and the plastids may be a general phenomenon in plants. © 2007 Springer-Verlag.
publishDate 2007
dc.date.none.fl_str_mv 2007-11-22
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/131848
Vargas, Walter Alberto; Pontis, Horacio Guillermo; Salerno, Graciela Lidia; New insights on sucrose metabolism: Evidence for an active A/N-Inv in chloroplasts uncovers a novel component of the intracellular carbon trafficking; Springer; Planta; 227; 4; 22-11-2007; 795-807
0032-0935
1432-2048
CONICET Digital
CONICET
url http://hdl.handle.net/11336/131848
identifier_str_mv Vargas, Walter Alberto; Pontis, Horacio Guillermo; Salerno, Graciela Lidia; New insights on sucrose metabolism: Evidence for an active A/N-Inv in chloroplasts uncovers a novel component of the intracellular carbon trafficking; Springer; Planta; 227; 4; 22-11-2007; 795-807
0032-0935
1432-2048
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/doi/10.1007/s00425-007-0657-1
info:eu-repo/semantics/altIdentifier/url/https://link.springer.com/article/10.1007/s00425-007-0657-1
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
application/pdf
application/pdf
dc.publisher.none.fl_str_mv Springer
publisher.none.fl_str_mv Springer
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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spelling New insights on sucrose metabolism: Evidence for an active A/N-Inv in chloroplasts uncovers a novel component of the intracellular carbon traffickingVargas, Walter AlbertoPontis, Horacio GuillermoSalerno, Graciela LidiaALKALINE/NEUTRAL INVERTASEARABIDOPSISCHLOROPLASTSSPINACH SUBCELLULAR LOCALIZATIONSUCROSE HYDROLYSIShttps://purl.org/becyt/ford/1.6https://purl.org/becyt/ford/1The presence of sucrose (Suc) in plastids was questioned for several decades. Although it was reported some decades ago, neither Suc transporters nor Suc metabolizing enzymes were demonstrated to be active in those organelles. By biochemical, immunological, molecular and genetic approaches we show that alkaline/neutral invertases (A/N-Invs) are also localized in chloroplasts of spinach and Arabidopsis. A/N-Inv activity and polypeptide content were shown in protein extracts from intact chloroplasts. Moreover, we functionally characterized the Arabidopsis At-A/N-InvE gene coding for a chloroplast-targeted A/N-Inv. The At-A/N-InvE knockout plants displayed a lower total A/N-Inv activity in comparison with wild-type plants. Furthermore, neither A/N-Inv activity nor A/N-Inv polypeptides were detected in protein extracts prepared from chloroplasts of mutant plants. Also, the measurement of carbohydrate content, in leaves harvested either at the end of the day or at the end of the night period, revealed that the knockout plants showed a decrease in starch accumulation but no alteration in Suc levels. These are the first results demonstrating the presence of a functional A/N-Inv inside chloroplasts and its relation with carbon storage in Arabidopsis leaves. Taken together our data and recent reports, we conclude that the participation of A/N-Invs in the carbon flux between the cytosol and the plastids may be a general phenomenon in plants. © 2007 Springer-Verlag.Fil: Vargas, Walter Alberto. Consejo Superior de Investigaciones Científicas. Centro de Investigaciones Biológicas; España. Fundación para Investigaciones Biológicas Aplicadas; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Parque Centenario; Argentina. Texas A&M University; Estados UnidosFil: Pontis, Horacio Guillermo. Consejo Superior de Investigaciones Científicas. Centro de Investigaciones Biológicas; España. Fundación para Investigaciones Biológicas Aplicadas; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Parque Centenario; ArgentinaFil: Salerno, Graciela Lidia. Consejo Superior de Investigaciones Científicas. Centro de Investigaciones Biológicas; España. Fundación para Investigaciones Biológicas Aplicadas; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Parque Centenario; ArgentinaSpringer2007-11-22info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/131848Vargas, Walter Alberto; Pontis, Horacio Guillermo; Salerno, Graciela Lidia; New insights on sucrose metabolism: Evidence for an active A/N-Inv in chloroplasts uncovers a novel component of the intracellular carbon trafficking; Springer; Planta; 227; 4; 22-11-2007; 795-8070032-09351432-2048CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/doi/10.1007/s00425-007-0657-1info:eu-repo/semantics/altIdentifier/url/https://link.springer.com/article/10.1007/s00425-007-0657-1info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2024-05-08T13:56:07Zoai:ri.conicet.gov.ar:11336/131848instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982024-05-08 13:56:07.491CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
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