Flunitrazepam induces geometrical changes at the lipid-water interface

Flunitrazepam (FNTZ) effects on molecular packing and surface curvature in artificial model membranes were investigated. FNTZ, from the subphase under dipalmitoylphosphatidylcholine (dpPC) monolayers at the air-water interface, expanded the surface pressure-area isotherm and induced an increment in...

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Detalhes bibliográficos
Autores: Garcia, Daniel Asmed, Perillo, Maria Angelica
Formato: artículo
Estado:Versión publicada
Fecha de publicación:2001
País:Argentina
Recursos:Consejo Nacional de Investigaciones Científicas y Técnicas
Repositorio:CONICET Digital (CONICET)
Idioma:inglés
OAI Identifier:oai:ri.conicet.gov.ar:11336/64526
Acesso em linha:http://hdl.handle.net/11336/64526
Access Level:acceso abierto
Palavra-chave:[3h]Gaba Entrapment
Aminophospholipid Translocation
Dipalmitoylphosphatidylcholine Self-Assembly
Electron Microscopy
Flunitrazepam
Monomolecular Layers
Multilamellar Vesicles
https://purl.org/becyt/ford/1.6
https://purl.org/becyt/ford/1
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oai_identifier_str oai:ri.conicet.gov.ar:11336/64526
network_acronym_str AR
network_name_str Argentina
repository_id_str
dc.title.none.fl_str_mv Flunitrazepam induces geometrical changes at the lipid-water interface
title Flunitrazepam induces geometrical changes at the lipid-water interface
spellingShingle Flunitrazepam induces geometrical changes at the lipid-water interface
Garcia, Daniel Asmed
[3h]Gaba Entrapment
Aminophospholipid Translocation
Dipalmitoylphosphatidylcholine Self-Assembly
Electron Microscopy
Flunitrazepam
Monomolecular Layers
Multilamellar Vesicles
https://purl.org/becyt/ford/1.6
https://purl.org/becyt/ford/1
title_short Flunitrazepam induces geometrical changes at the lipid-water interface
title_full Flunitrazepam induces geometrical changes at the lipid-water interface
title_fullStr Flunitrazepam induces geometrical changes at the lipid-water interface
title_full_unstemmed Flunitrazepam induces geometrical changes at the lipid-water interface
title_sort Flunitrazepam induces geometrical changes at the lipid-water interface
dc.creator.none.fl_str_mv Garcia, Daniel Asmed
Perillo, Maria Angelica
author Garcia, Daniel Asmed
author_facet Garcia, Daniel Asmed
Perillo, Maria Angelica
author_role author
author2 Perillo, Maria Angelica
author2_role author
dc.subject.none.fl_str_mv [3h]Gaba Entrapment
Aminophospholipid Translocation
Dipalmitoylphosphatidylcholine Self-Assembly
Electron Microscopy
Flunitrazepam
Monomolecular Layers
Multilamellar Vesicles
https://purl.org/becyt/ford/1.6
https://purl.org/becyt/ford/1
topic [3h]Gaba Entrapment
Aminophospholipid Translocation
Dipalmitoylphosphatidylcholine Self-Assembly
Electron Microscopy
Flunitrazepam
Monomolecular Layers
Multilamellar Vesicles
https://purl.org/becyt/ford/1.6
https://purl.org/becyt/ford/1
description Flunitrazepam (FNTZ) effects on molecular packing and surface curvature in artificial model membranes were investigated. FNTZ, from the subphase under dipalmitoylphosphatidylcholine (dpPC) monolayers at the air-water interface, expanded the surface pressure-area isotherm and induced an increment in the limiting area; in this conditions, the collapse pressure of dpPC decreased, indicating a lowering in the stability of the monolayer. Thermodynamic-geometric correlations based on molecular parameters predicted a decrement in the aggregation number and stability, and an increase in the curvature of the self-aggregated structure of dpPC in aqueous medium in the presence of FNTZ. Accordingly, negative-staining electron microscopy of dpPC aqueous dispersions showed that the mean diameter of dpPC vesicles decreased 2 and 2.87 times in the presence of 10 nM and 50 μM FNTZ, respectively, compared with control samples. The release of a soluble marker entrapped in dpPC liposomes increased slightly respect to the control in the presence of FNTZ. In dpPC-dpPE mixed liposomes 50 μM FNTZ induced a decrement in the amount of the aminophospholipid exposed to the outer monolayer. Concluding, an FNTZ-induced expansion of dpPC-water interface region affected the constraints imposed on the lipid-water system by the molecular geometry, interacting free energies and entropy that determine the shape of a multimolecular structure. In liposomes composed of a pure phospholipid, the bilayer expansion leaded, through a structure instability, to reduce the liposome size; in mixed liposomes, phospholipid molecules translocation could be observed as another compensating mechanism of the initial perturbation. These results may be relevant for understanding benzodiazepines' effects non-mediated by membrane receptors. (C) 2001 Elsevier Science B.V.
publishDate 2001
dc.date.none.fl_str_mv 2001-01-15
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/64526
Garcia, Daniel Asmed; Perillo, Maria Angelica; Flunitrazepam induces geometrical changes at the lipid-water interface; Elsevier Science; Colloids and Surfaces B: Biointerfaces; 20; 1; 15-1-2001; 63-72
0927-7765
CONICET Digital
CONICET
url http://hdl.handle.net/11336/64526
identifier_str_mv Garcia, Daniel Asmed; Perillo, Maria Angelica; Flunitrazepam induces geometrical changes at the lipid-water interface; Elsevier Science; Colloids and Surfaces B: Biointerfaces; 20; 1; 15-1-2001; 63-72
0927-7765
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/url/https://www.sciencedirect.com/science/article/pii/S0927776500001715
info:eu-repo/semantics/altIdentifier/doi/10.1016/S0927-7765(00)00171-5
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
dc.publisher.none.fl_str_mv Elsevier Science
publisher.none.fl_str_mv Elsevier Science
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
_version_ 1799195723574542336
spelling Flunitrazepam induces geometrical changes at the lipid-water interfaceGarcia, Daniel AsmedPerillo, Maria Angelica[3h]Gaba EntrapmentAminophospholipid TranslocationDipalmitoylphosphatidylcholine Self-AssemblyElectron MicroscopyFlunitrazepamMonomolecular LayersMultilamellar Vesicleshttps://purl.org/becyt/ford/1.6https://purl.org/becyt/ford/1Flunitrazepam (FNTZ) effects on molecular packing and surface curvature in artificial model membranes were investigated. FNTZ, from the subphase under dipalmitoylphosphatidylcholine (dpPC) monolayers at the air-water interface, expanded the surface pressure-area isotherm and induced an increment in the limiting area; in this conditions, the collapse pressure of dpPC decreased, indicating a lowering in the stability of the monolayer. Thermodynamic-geometric correlations based on molecular parameters predicted a decrement in the aggregation number and stability, and an increase in the curvature of the self-aggregated structure of dpPC in aqueous medium in the presence of FNTZ. Accordingly, negative-staining electron microscopy of dpPC aqueous dispersions showed that the mean diameter of dpPC vesicles decreased 2 and 2.87 times in the presence of 10 nM and 50 μM FNTZ, respectively, compared with control samples. The release of a soluble marker entrapped in dpPC liposomes increased slightly respect to the control in the presence of FNTZ. In dpPC-dpPE mixed liposomes 50 μM FNTZ induced a decrement in the amount of the aminophospholipid exposed to the outer monolayer. Concluding, an FNTZ-induced expansion of dpPC-water interface region affected the constraints imposed on the lipid-water system by the molecular geometry, interacting free energies and entropy that determine the shape of a multimolecular structure. In liposomes composed of a pure phospholipid, the bilayer expansion leaded, through a structure instability, to reduce the liposome size; in mixed liposomes, phospholipid molecules translocation could be observed as another compensating mechanism of the initial perturbation. These results may be relevant for understanding benzodiazepines' effects non-mediated by membrane receptors. (C) 2001 Elsevier Science B.V.Fil: Garcia, Daniel Asmed. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba. Instituto de Investigaciones Biológicas y Tecnológicas. Universidad Nacional de Córdoba. Facultad de Ciencias Exactas, Físicas y Naturales. Instituto de Investigaciones Biológicas y Tecnológicas; ArgentinaFil: Perillo, Maria Angelica. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba. Instituto de Investigaciones Biológicas y Tecnológicas. Universidad Nacional de Córdoba. Facultad de Ciencias Exactas, Físicas y Naturales. Instituto de Investigaciones Biológicas y Tecnológicas; ArgentinaElsevier Science2001-01-15info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/64526Garcia, Daniel Asmed; Perillo, Maria Angelica; Flunitrazepam induces geometrical changes at the lipid-water interface; Elsevier Science; Colloids and Surfaces B: Biointerfaces; 20; 1; 15-1-2001; 63-720927-7765CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/url/https://www.sciencedirect.com/science/article/pii/S0927776500001715info:eu-repo/semantics/altIdentifier/doi/10.1016/S0927-7765(00)00171-5info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2024-05-08T14:01:29Zoai:ri.conicet.gov.ar:11336/64526instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982024-05-08 14:01:30.112CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
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