Formation and nanoscale characterization of asymmetric supported lipid bilayers containing raft-like domains
The development of new strategies for achieving stable asymmetric membrane models has turned interleaflet lipid asymmetry into a topic of major interest. Cyclodextrin-mediated lipid exchange constitutes a simple and versatile method for preparing asymmetric membrane models without the need for sophi...
| Autores: | , , , , , |
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| Formato: | artículo |
| Estado: | Versión publicada |
| Fecha de publicación: | 2022 |
| País: | Argentina |
| Recursos: | Consejo Nacional de Investigaciones Científicas y Técnicas |
| Repositorio: | CONICET Digital (CONICET) |
| Idioma: | inglés |
| OAI Identifier: | oai:ri.conicet.gov.ar:11336/214559 |
| Acesso em linha: | http://hdl.handle.net/11336/214559 |
| Access Level: | acceso abierto |
| Palavra-chave: | ATOMIC FORCE MICROSCOPY-FORCE SPECTROSCOPY CYCLODEXTRINS LIPID DOMAINS LIPID EXCHANGE MEMBRANE ASYMMETRY SUPPORTED LIPID BILAYERS https://purl.org/becyt/ford/1.6 https://purl.org/becyt/ford/1 |
| Resumo: | The development of new strategies for achieving stable asymmetric membrane models has turned interleaflet lipid asymmetry into a topic of major interest. Cyclodextrin-mediated lipid exchange constitutes a simple and versatile method for preparing asymmetric membrane models without the need for sophisticated equipment. Here we describe a protocol for preparing asymmetric supported lipid bilayers mimicking membrane rafts by cyclodextrin-mediated lipid exchange and the main guidelines for obtaining structural information and quantitative measures of their mechanical properties using Atomic force microscopy and Force spectroscopy; two powerful techniques that allow membrane characterization at the nanoscale. |
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