Arsenic metabolism in high altitude modern stromatolites revealed by metagenomic analysis

Modern stromatolites thrive only in selected locations in the world. Socompa Lake, located in the Andean plateau at 3570 masl, is one of the numerous extreme Andean microbial ecosystems described over recent years. Extreme environmental conditions include hypersalinity, high UV incidence, and high a...

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Detalhes bibliográficos
Autores: Kurth, Daniel German, Amadio, Ariel Fernando, Ordoñez, Omar Federico, Albarracín, Virginia Helena, Gärtner, Wolfgang, Farias, Maria Eugenia
Formato: artículo
Estado:Versión publicada
Fecha de publicación:2017
País:Argentina
Recursos:Consejo Nacional de Investigaciones Científicas y Técnicas
Repositorio:CONICET Digital (CONICET)
Idioma:inglés
OAI Identifier:oai:ri.conicet.gov.ar:11336/63437
Acesso em linha:http://hdl.handle.net/11336/63437
Access Level:acceso abierto
Palavra-chave:METAGENOMICS
DNA SEQUENCING
MICROBIAL MATS
ARSENIC
https://purl.org/becyt/ford/1.6
https://purl.org/becyt/ford/1
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network_acronym_str AR
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repository_id_str
dc.title.none.fl_str_mv Arsenic metabolism in high altitude modern stromatolites revealed by metagenomic analysis
title Arsenic metabolism in high altitude modern stromatolites revealed by metagenomic analysis
spellingShingle Arsenic metabolism in high altitude modern stromatolites revealed by metagenomic analysis
Kurth, Daniel German
METAGENOMICS
DNA SEQUENCING
MICROBIAL MATS
ARSENIC
https://purl.org/becyt/ford/1.6
https://purl.org/becyt/ford/1
title_short Arsenic metabolism in high altitude modern stromatolites revealed by metagenomic analysis
title_full Arsenic metabolism in high altitude modern stromatolites revealed by metagenomic analysis
title_fullStr Arsenic metabolism in high altitude modern stromatolites revealed by metagenomic analysis
title_full_unstemmed Arsenic metabolism in high altitude modern stromatolites revealed by metagenomic analysis
title_sort Arsenic metabolism in high altitude modern stromatolites revealed by metagenomic analysis
dc.creator.none.fl_str_mv Kurth, Daniel German
Amadio, Ariel Fernando
Ordoñez, Omar Federico
Albarracín, Virginia Helena
Gärtner, Wolfgang
Farias, Maria Eugenia
author Kurth, Daniel German
author_facet Kurth, Daniel German
Amadio, Ariel Fernando
Ordoñez, Omar Federico
Albarracín, Virginia Helena
Gärtner, Wolfgang
Farias, Maria Eugenia
author_role author
author2 Amadio, Ariel Fernando
Ordoñez, Omar Federico
Albarracín, Virginia Helena
Gärtner, Wolfgang
Farias, Maria Eugenia
author2_role author
author
author
author
author
dc.subject.none.fl_str_mv METAGENOMICS
DNA SEQUENCING
MICROBIAL MATS
ARSENIC
https://purl.org/becyt/ford/1.6
https://purl.org/becyt/ford/1
topic METAGENOMICS
DNA SEQUENCING
MICROBIAL MATS
ARSENIC
https://purl.org/becyt/ford/1.6
https://purl.org/becyt/ford/1
description Modern stromatolites thrive only in selected locations in the world. Socompa Lake, located in the Andean plateau at 3570 masl, is one of the numerous extreme Andean microbial ecosystems described over recent years. Extreme environmental conditions include hypersalinity, high UV incidence, and high arsenic content, among others. After Socompa's stromatolite microbial communities were analysed by metagenomic DNA sequencing, taxonomic classification showed dominance of Proteobacteria, Bacteroidetes and Firmicutes, and a remarkably high number of unclassified sequences. A functional analysis indicated that carbon fixation might occur not only by the Calvin-Benson cycle, but also through alternative pathways such as the reverse TCA cycle, and the reductive acetyl-CoA pathway. Deltaproteobacteria were involved both in sulfate reduction and nitrogen fixation. Significant differences were found when comparing the Socompa stromatolite metagenome to the Shark Bay (Australia) smooth mat metagenome: namely, those involving stress related processes, particularly, arsenic resistance. An in-depth analysis revealed a surprisingly diverse metabolism comprising all known types of As resistance and energy generating pathways. While the ars operon was the main mechanism, an important abundance of arsM genes was observed in selected phyla. The data resulting from this work will prove a cornerstone for further studies on this rare microbial community.
publishDate 2017
dc.date.none.fl_str_mv 2017-12
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/63437
Kurth, Daniel German; Amadio, Ariel Fernando; Ordoñez, Omar Federico; Albarracín, Virginia Helena; Gärtner, Wolfgang; et al.; Arsenic metabolism in high altitude modern stromatolites revealed by metagenomic analysis; Nature Publishing Group; Scientific Reports; 7; 1; 12-2017; 1-16
2045-2322
CONICET Digital
CONICET
url http://hdl.handle.net/11336/63437
identifier_str_mv Kurth, Daniel German; Amadio, Ariel Fernando; Ordoñez, Omar Federico; Albarracín, Virginia Helena; Gärtner, Wolfgang; et al.; Arsenic metabolism in high altitude modern stromatolites revealed by metagenomic analysis; Nature Publishing Group; Scientific Reports; 7; 1; 12-2017; 1-16
2045-2322
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/doi/10.1038/s41598-017-00896-0
info:eu-repo/semantics/altIdentifier/url/https://www.nature.com/articles/s41598-017-00896-0
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
application/pdf
application/pdf
application/pdf
application/pdf
dc.publisher.none.fl_str_mv Nature Publishing Group
publisher.none.fl_str_mv Nature Publishing Group
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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spelling Arsenic metabolism in high altitude modern stromatolites revealed by metagenomic analysisKurth, Daniel GermanAmadio, Ariel FernandoOrdoñez, Omar FedericoAlbarracín, Virginia HelenaGärtner, WolfgangFarias, Maria EugeniaMETAGENOMICSDNA SEQUENCINGMICROBIAL MATSARSENIChttps://purl.org/becyt/ford/1.6https://purl.org/becyt/ford/1Modern stromatolites thrive only in selected locations in the world. Socompa Lake, located in the Andean plateau at 3570 masl, is one of the numerous extreme Andean microbial ecosystems described over recent years. Extreme environmental conditions include hypersalinity, high UV incidence, and high arsenic content, among others. After Socompa's stromatolite microbial communities were analysed by metagenomic DNA sequencing, taxonomic classification showed dominance of Proteobacteria, Bacteroidetes and Firmicutes, and a remarkably high number of unclassified sequences. A functional analysis indicated that carbon fixation might occur not only by the Calvin-Benson cycle, but also through alternative pathways such as the reverse TCA cycle, and the reductive acetyl-CoA pathway. Deltaproteobacteria were involved both in sulfate reduction and nitrogen fixation. Significant differences were found when comparing the Socompa stromatolite metagenome to the Shark Bay (Australia) smooth mat metagenome: namely, those involving stress related processes, particularly, arsenic resistance. An in-depth analysis revealed a surprisingly diverse metabolism comprising all known types of As resistance and energy generating pathways. While the ars operon was the main mechanism, an important abundance of arsM genes was observed in selected phyla. The data resulting from this work will prove a cornerstone for further studies on this rare microbial community.Fil: Kurth, Daniel German. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Planta Piloto de Procesos Industriales Microbiológicos; ArgentinaFil: Amadio, Ariel Fernando. Instituto Nacional de Tecnología Agropecuaria. Centro Regional Santa Fe. Estación Experimental Agropecuaria Rafaela; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaFil: Ordoñez, Omar Federico. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Planta Piloto de Procesos Industriales Microbiológicos; ArgentinaFil: Albarracín, Virginia Helena. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Planta Piloto de Procesos Industriales Microbiológicos; ArgentinaFil: Gärtner, Wolfgang. Max-Planck Institute for Chemical Energy Conversion. Mülheim an der Ruhr; AlemaniaFil: Farias, Maria Eugenia. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Planta Piloto de Procesos Industriales Microbiológicos; ArgentinaNature Publishing Group2017-12info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfapplication/pdfapplication/pdfapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/63437Kurth, Daniel German; Amadio, Ariel Fernando; Ordoñez, Omar Federico; Albarracín, Virginia Helena; Gärtner, Wolfgang; et al.; Arsenic metabolism in high altitude modern stromatolites revealed by metagenomic analysis; Nature Publishing Group; Scientific Reports; 7; 1; 12-2017; 1-162045-2322CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/doi/10.1038/s41598-017-00896-0info:eu-repo/semantics/altIdentifier/url/https://www.nature.com/articles/s41598-017-00896-0info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2024-05-08T13:37:54Zoai:ri.conicet.gov.ar:11336/63437instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982024-05-08 13:37:55.256CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
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