Mammalian Staufen 1 is recruited to stress granules and impairs their assembly
Stress granules are cytoplasmic mRNA-silencing foci that form transiently during the stress response. Stress granules harbor abortive translation initiation complexes and are in dynamic equilibrium with translating polysomes. Mammalian Staufen 1 (Stau1) is a ubiquitous double-stranded RNA-binding pr...
| Autores: | , , , , |
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| Tipo de recurso: | artículo |
| Estado: | Versión publicada |
| Fecha de publicación: | 2009 |
| País: | Argentina |
| Institución: | Universidad Nacional de Buenos Aires. Facultad de Ciencias Exactas y Naturales |
| Repositorio: | Biblioteca Digital (UBA-FCEN) |
| Idioma: | inglés |
| OAI Identifier: | paperaa:paper_00219533_v122_n4_p563_Thomas |
| Acceso en línea: | http://hdl.handle.net/20.500.12110/paper_00219533_v122_n4_p563_Thomas |
| Access Level: | acceso abierto |
| Palabra clave: | ER stress Oxidative stress P bodies Silencing foci Staufen Stress granules initiation factor 2alpha RNA binding protein staufen 1 unclassified drug enzyme inhibitor heat shock protein 70 initiation factor 2 small interfering RNA staufen protein, mammalian thapsigargin animal cell article cell granule cellular stress response controlled study endoplasmic reticulum stress gene overexpression gene silencing human human cell nonhuman oxidative stress polysome priority journal protein assembly protein phosphorylation regulatory mechanism translation initiation animal biosynthesis cell strain 3T3 cell strain COS1 Cercopithecus chemistry confocal microscopy drug effect endoplasmic reticulum HeLa cell metabolism mouse physiological stress physiology protein synthesis protein tertiary structure rat ultrastructure Mammalia Animals Cercopithecus aethiops COS Cells Cytoplasmic Granules Endoplasmic Reticulum Enzyme Inhibitors Eukaryotic Initiation Factor-2 Hela Cells HSP70 Heat-Shock Proteins Humans Mice Microscopy, Confocal NIH 3T3 Cells Oxidative Stress Polyribosomes Protein Biosynthesis Protein Structure, Tertiary Rats RNA, Small Interfering RNA-Binding Proteins Stress, Physiological Thapsigargin |
| Sumario: | Stress granules are cytoplasmic mRNA-silencing foci that form transiently during the stress response. Stress granules harbor abortive translation initiation complexes and are in dynamic equilibrium with translating polysomes. Mammalian Staufen 1 (Stau1) is a ubiquitous double-stranded RNA-binding protein associated with polysomes. Here, we show that Stau1 is recruited to stress granules upon induction of endoplasmic reticulum or oxidative stress as well in stress granules induced by translation initiation blockers. We found that stress granules lacking Stau1 formed in cells depleted of this molecule, indicating that Stau1 is not an essential component of stress granules. Moreover, Stau1 knockdown facilitated stress granule formation upon stress induction. Conversely, transient transfection of Stau1 impaired stress granule formation upon stress or pharmacological initiation arrest. The inhibitory capacity of Stau1 mapped to the amino-terminal half of the molecule, a region known to bind to polysomes. We found that the fraction of polysomes remaining upon stress induction was enriched in Stau1, and that Stau1 overexpression stabilized polysomes against stress. We propose that Stau1 is involved in recovery from stress by stabilizing polysomes, thus helping stress granule dissolution. |
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