Intranasal administration of TLR agonists induces a discriminated local innate response along murine respiratory tract

Adjuvants are relevant for mucosal immunization in order to induce long lasting protective immunity. It has been shown that targeting to different regions of the airway results in different capacity to trigger adaptive/protective immunity. Nevertheless there is scarce knowledge regarding topological...

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Detalhes bibliográficos
Autores: Errea, Agustina Juliana, González Maciel, María Dolores, Hiriart, Yanina, Hozbor, Daniela Flavia, Rumbo, Martín
Formato: artículo
Estado:Versión publicada
Fecha de publicación:2015
País:Argentina
Recursos:Consejo Nacional de Investigaciones Científicas y Técnicas
Repositorio:CONICET Digital (CONICET)
Idioma:inglés
OAI Identifier:oai:ri.conicet.gov.ar:11336/45801
Acesso em linha:http://hdl.handle.net/11336/45801
Access Level:acceso abierto
Palavra-chave:Toll Like Receptors
Tlr Agonist
Airways
Innate Response
https://purl.org/becyt/ford/3.1
https://purl.org/becyt/ford/3
id AR_84eb84e972ec6a2bd75b93fe15fefdc4
oai_identifier_str oai:ri.conicet.gov.ar:11336/45801
network_acronym_str AR
network_name_str Argentina
repository_id_str
dc.title.none.fl_str_mv Intranasal administration of TLR agonists induces a discriminated local innate response along murine respiratory tract
title Intranasal administration of TLR agonists induces a discriminated local innate response along murine respiratory tract
spellingShingle Intranasal administration of TLR agonists induces a discriminated local innate response along murine respiratory tract
Errea, Agustina Juliana
Toll Like Receptors
Tlr Agonist
Airways
Innate Response
https://purl.org/becyt/ford/3.1
https://purl.org/becyt/ford/3
title_short Intranasal administration of TLR agonists induces a discriminated local innate response along murine respiratory tract
title_full Intranasal administration of TLR agonists induces a discriminated local innate response along murine respiratory tract
title_fullStr Intranasal administration of TLR agonists induces a discriminated local innate response along murine respiratory tract
title_full_unstemmed Intranasal administration of TLR agonists induces a discriminated local innate response along murine respiratory tract
title_sort Intranasal administration of TLR agonists induces a discriminated local innate response along murine respiratory tract
dc.creator.none.fl_str_mv Errea, Agustina Juliana
González Maciel, María Dolores
Hiriart, Yanina
Hozbor, Daniela Flavia
Rumbo, Martín
author Errea, Agustina Juliana
author_facet Errea, Agustina Juliana
González Maciel, María Dolores
Hiriart, Yanina
Hozbor, Daniela Flavia
Rumbo, Martín
author_role author
author2 González Maciel, María Dolores
Hiriart, Yanina
Hozbor, Daniela Flavia
Rumbo, Martín
author2_role author
author
author
author
dc.subject.none.fl_str_mv Toll Like Receptors
Tlr Agonist
Airways
Innate Response
https://purl.org/becyt/ford/3.1
https://purl.org/becyt/ford/3
topic Toll Like Receptors
Tlr Agonist
Airways
Innate Response
https://purl.org/becyt/ford/3.1
https://purl.org/becyt/ford/3
description Adjuvants are relevant for mucosal immunization in order to induce long lasting protective immunity. It has been shown that targeting to different regions of the airway results in different capacity to trigger adaptive/protective immunity. Nevertheless there is scarce knowledge regarding topological responsiveness along airways to TLR agonists. We analyzed the effects of intranasal administration of lipopolysaccharide (LPS), poly I:C and flagellin on the expression of a panel of innate response markers along murine airways by laser microdissection and RTqPCR. In all cases treatment induced recruitment of inflammatory cells to airways. However, regional gene expression indicated that whereas deeper airways (mainly alveoli) respond with high expression of IL6, CXCL1 and CXCL10, the response in conductive airways (bronchi and bronchioles) is dominated by expression of CCL20. On the other hand, triggering TLR3 elicits a response dominated by CXCL10, showing higher expression at 6 h compared to 2 h, whereas LPS and flagellin induce a response peaking at 2 h and dominated by IL6 and CXCL1. The results presented here showed difference in topological response triggered by different TLR agonist. These results make the targeting of different sites of airways a variable to evaluate when selecting the appropriate combinations of TLR and vaccinal antigens for intranasal delivery.
publishDate 2015
dc.date.none.fl_str_mv 2015-03
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/45801
Errea, Agustina Juliana; González Maciel, María Dolores; Hiriart, Yanina; Hozbor, Daniela Flavia; Rumbo, Martín; Intranasal administration of TLR agonists induces a discriminated local innate response along murine respiratory tract; Elsevier Science; Immunology Letters; 164; 3-2015; 33-39
0165-2478
CONICET Digital
CONICET
url http://hdl.handle.net/11336/45801
identifier_str_mv Errea, Agustina Juliana; González Maciel, María Dolores; Hiriart, Yanina; Hozbor, Daniela Flavia; Rumbo, Martín; Intranasal administration of TLR agonists induces a discriminated local innate response along murine respiratory tract; Elsevier Science; Immunology Letters; 164; 3-2015; 33-39
0165-2478
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/doi/10.1016/j.imlet.2015.01.004
info:eu-repo/semantics/altIdentifier/url/https://linkinghub.elsevier.com/retrieve/pii/S016524781500005X
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
application/pdf
dc.publisher.none.fl_str_mv Elsevier Science
publisher.none.fl_str_mv Elsevier Science
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
_version_ 1799196273209769984
spelling Intranasal administration of TLR agonists induces a discriminated local innate response along murine respiratory tractErrea, Agustina JulianaGonzález Maciel, María DoloresHiriart, YaninaHozbor, Daniela FlaviaRumbo, MartínToll Like ReceptorsTlr AgonistAirwaysInnate Responsehttps://purl.org/becyt/ford/3.1https://purl.org/becyt/ford/3Adjuvants are relevant for mucosal immunization in order to induce long lasting protective immunity. It has been shown that targeting to different regions of the airway results in different capacity to trigger adaptive/protective immunity. Nevertheless there is scarce knowledge regarding topological responsiveness along airways to TLR agonists. We analyzed the effects of intranasal administration of lipopolysaccharide (LPS), poly I:C and flagellin on the expression of a panel of innate response markers along murine airways by laser microdissection and RTqPCR. In all cases treatment induced recruitment of inflammatory cells to airways. However, regional gene expression indicated that whereas deeper airways (mainly alveoli) respond with high expression of IL6, CXCL1 and CXCL10, the response in conductive airways (bronchi and bronchioles) is dominated by expression of CCL20. On the other hand, triggering TLR3 elicits a response dominated by CXCL10, showing higher expression at 6 h compared to 2 h, whereas LPS and flagellin induce a response peaking at 2 h and dominated by IL6 and CXCL1. The results presented here showed difference in topological response triggered by different TLR agonist. These results make the targeting of different sites of airways a variable to evaluate when selecting the appropriate combinations of TLR and vaccinal antigens for intranasal delivery.Fil: Errea, Agustina Juliana. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata. Instituto de Estudios Inmunológicos y Fisiopatológicos. Universidad Nacional de La Plata. Facultad de Ciencias Exactas. Instituto de Estudios Inmunológicos y Fisiopatológicos; ArgentinaFil: González Maciel, María Dolores. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata. Instituto de Estudios Inmunológicos y Fisiopatológicos. Universidad Nacional de La Plata. Facultad de Ciencias Exactas. Instituto de Estudios Inmunológicos y Fisiopatológicos; ArgentinaFil: Hiriart, Yanina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata. Instituto de Estudios Inmunológicos y Fisiopatológicos. Universidad Nacional de La Plata. Facultad de Ciencias Exactas. Instituto de Estudios Inmunológicos y Fisiopatológicos; ArgentinaFil: Hozbor, Daniela Flavia. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata. Instituto de Biotecnología y Biología Molecular. Universidad Nacional de La Plata. Facultad de Ciencias Exactas. Instituto de Biotecnología y Biología Molecular; ArgentinaFil: Rumbo, Martín. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata. Instituto de Estudios Inmunológicos y Fisiopatológicos. Universidad Nacional de La Plata. Facultad de Ciencias Exactas. Instituto de Estudios Inmunológicos y Fisiopatológicos; ArgentinaElsevier Science2015-03info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/45801Errea, Agustina Juliana; González Maciel, María Dolores; Hiriart, Yanina; Hozbor, Daniela Flavia; Rumbo, Martín; Intranasal administration of TLR agonists induces a discriminated local innate response along murine respiratory tract; Elsevier Science; Immunology Letters; 164; 3-2015; 33-390165-2478CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/doi/10.1016/j.imlet.2015.01.004info:eu-repo/semantics/altIdentifier/url/https://linkinghub.elsevier.com/retrieve/pii/S016524781500005Xinfo:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2024-05-08T14:21:10Zoai:ri.conicet.gov.ar:11336/45801instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982024-05-08 14:21:10.747CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
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