Ultrasensitive behavior in the synthesis of storage polysaccharides in cyanobacteria

The glycogen synthetic pathway operates ultrasensitively as a function of the ADPglucose pyrophosphorylase (ADPGlcPPase) allosteric effectors, 3-phosphoglycerate and Pi, in permeabilized cells of the cyanobacterium Anabaena PCC 7120. In vitro data previously showed that the ultrasensitive behavior o...

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Detalles Bibliográficos
Autores: Gomez Casati, Diego Fabian, Cortassa, Sonia del Carmen, Aon, Miguel A., Iglesias, Alberto Alvaro
Tipo de recurso: artículo
Estado:Versión publicada
Fecha de publicación:2003
País:Argentina
Institución:Consejo Nacional de Investigaciones Científicas y Técnicas
Repositorio:CONICET Digital (CONICET)
Idioma:inglés
OAI Identifier:oai:ri.conicet.gov.ar:11336/93436
Acceso en línea:http://hdl.handle.net/11336/93436
Access Level:acceso abierto
Palabra clave:Permeabilized cyanobacteria
ADPGLUCOSE PYROPHOSPHORYLASE
ANABAENA
MOLECULAR CROWDING
PERMEABILIZED CYANOBACTERIA
https://purl.org/becyt/ford/1.6
https://purl.org/becyt/ford/1
id AR_83d5c044ceaf7c33bca8df48a68fbcb4
oai_identifier_str oai:ri.conicet.gov.ar:11336/93436
network_acronym_str AR
network_name_str Argentina
repository_id_str
dc.title.none.fl_str_mv Ultrasensitive behavior in the synthesis of storage polysaccharides in cyanobacteria
title Ultrasensitive behavior in the synthesis of storage polysaccharides in cyanobacteria
spellingShingle Ultrasensitive behavior in the synthesis of storage polysaccharides in cyanobacteria
Gomez Casati, Diego Fabian
Permeabilized cyanobacteria
ADPGLUCOSE PYROPHOSPHORYLASE
ANABAENA
MOLECULAR CROWDING
PERMEABILIZED CYANOBACTERIA
https://purl.org/becyt/ford/1.6
https://purl.org/becyt/ford/1
title_short Ultrasensitive behavior in the synthesis of storage polysaccharides in cyanobacteria
title_full Ultrasensitive behavior in the synthesis of storage polysaccharides in cyanobacteria
title_fullStr Ultrasensitive behavior in the synthesis of storage polysaccharides in cyanobacteria
title_full_unstemmed Ultrasensitive behavior in the synthesis of storage polysaccharides in cyanobacteria
title_sort Ultrasensitive behavior in the synthesis of storage polysaccharides in cyanobacteria
dc.creator.none.fl_str_mv Gomez Casati, Diego Fabian
Cortassa, Sonia del Carmen
Aon, Miguel A.
Iglesias, Alberto Alvaro
author Gomez Casati, Diego Fabian
author_facet Gomez Casati, Diego Fabian
Cortassa, Sonia del Carmen
Aon, Miguel A.
Iglesias, Alberto Alvaro
author_role author
author2 Cortassa, Sonia del Carmen
Aon, Miguel A.
Iglesias, Alberto Alvaro
author2_role author
author
author
dc.subject.none.fl_str_mv Permeabilized cyanobacteria
ADPGLUCOSE PYROPHOSPHORYLASE
ANABAENA
MOLECULAR CROWDING
PERMEABILIZED CYANOBACTERIA
https://purl.org/becyt/ford/1.6
https://purl.org/becyt/ford/1
topic Permeabilized cyanobacteria
ADPGLUCOSE PYROPHOSPHORYLASE
ANABAENA
MOLECULAR CROWDING
PERMEABILIZED CYANOBACTERIA
https://purl.org/becyt/ford/1.6
https://purl.org/becyt/ford/1
description The glycogen synthetic pathway operates ultrasensitively as a function of the ADPglucose pyrophosphorylase (ADPGlcPPase) allosteric effectors, 3-phosphoglycerate and Pi, in permeabilized cells of the cyanobacterium Anabaena PCC 7120. In vitro data previously showed that the ultrasensitive behavior of ADPGlcPPase depends upon cross-talk between the two allosteric effectors, the enzyme's response being additionally modulated by molecular crowding [D.F. Gómez Casatiet al. (2000) Biochem J 350:139–147]. In the present work we show, experimentally and with a mathematical model, that α-1,4-glucan synthesis is also ultrasensitive in cells due to the propagation of the switch-like behavior of ADPGlcPPase to the synthetic pathway. Amplifications of up to 20-fold in storage-polysaccharide synthesis can be achieved with a modest 6.7-fold increase in 3-phosphoglycerate in the presence of 5 mM Pi in contrast to the 30-fold necessary in its absence. This is the first time that this phenomenon has been reported to occur in the glycogen synthetic pathway of a photosynthetic prokaryote. The implications of the results for plant cell physiology during light–dark transitions are discussed.
publishDate 2003
dc.date.none.fl_str_mv 2003-04
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/93436
Gomez Casati, Diego Fabian; Cortassa, Sonia del Carmen; Aon, Miguel A.; Iglesias, Alberto Alvaro; Ultrasensitive behavior in the synthesis of storage polysaccharides in cyanobacteria; Springer Heidelberg; Planta; 216; 6; 4-2003; 969-975
0032-0935
1432-2048
CONICET Digital
CONICET
url http://hdl.handle.net/11336/93436
identifier_str_mv Gomez Casati, Diego Fabian; Cortassa, Sonia del Carmen; Aon, Miguel A.; Iglesias, Alberto Alvaro; Ultrasensitive behavior in the synthesis of storage polysaccharides in cyanobacteria; Springer Heidelberg; Planta; 216; 6; 4-2003; 969-975
0032-0935
1432-2048
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/url/https://link.springer.com/article/10.1007/s00425-002-0949-4
info:eu-repo/semantics/altIdentifier/doi/10.1007/s00425-002-0949-4
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
application/pdf
dc.publisher.none.fl_str_mv Springer Heidelberg
publisher.none.fl_str_mv Springer Heidelberg
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
_version_ 1799195406780858368
spelling Ultrasensitive behavior in the synthesis of storage polysaccharides in cyanobacteriaGomez Casati, Diego FabianCortassa, Sonia del CarmenAon, Miguel A.Iglesias, Alberto AlvaroPermeabilized cyanobacteriaADPGLUCOSE PYROPHOSPHORYLASEANABAENAMOLECULAR CROWDINGPERMEABILIZED CYANOBACTERIAhttps://purl.org/becyt/ford/1.6https://purl.org/becyt/ford/1The glycogen synthetic pathway operates ultrasensitively as a function of the ADPglucose pyrophosphorylase (ADPGlcPPase) allosteric effectors, 3-phosphoglycerate and Pi, in permeabilized cells of the cyanobacterium Anabaena PCC 7120. In vitro data previously showed that the ultrasensitive behavior of ADPGlcPPase depends upon cross-talk between the two allosteric effectors, the enzyme's response being additionally modulated by molecular crowding [D.F. Gómez Casatiet al. (2000) Biochem J 350:139–147]. In the present work we show, experimentally and with a mathematical model, that α-1,4-glucan synthesis is also ultrasensitive in cells due to the propagation of the switch-like behavior of ADPGlcPPase to the synthetic pathway. Amplifications of up to 20-fold in storage-polysaccharide synthesis can be achieved with a modest 6.7-fold increase in 3-phosphoglycerate in the presence of 5 mM Pi in contrast to the 30-fold necessary in its absence. This is the first time that this phenomenon has been reported to occur in the glycogen synthetic pathway of a photosynthetic prokaryote. The implications of the results for plant cell physiology during light–dark transitions are discussed.Fil: Gomez Casati, Diego Fabian. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Rosario. Centro de Estudios Fotosintéticos y Bioquímicos. Universidad Nacional de Rosario. Facultad de Ciencias Bioquímicas y Farmacéuticas. Centro de Estudios Fotosintéticos y Bioquímicos; ArgentinaFil: Cortassa, Sonia del Carmen. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata. Instituto de Investigaciones Biotecnológicas. Instituto de Investigaciones Biotecnológicas "Dr. Raúl Alfonsín" (sede Chascomús). Universidad Nacional de San Martín. Instituto de Investigaciones Biotecnológicas. Instituto de Investigaciones Biotecnológicas "Dr. Raúl Alfonsín" (sede Chascomús); ArgentinaFil: Aon, Miguel. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata. Instituto de Investigaciones Biotecnológicas. Instituto de Investigaciones Biotecnológicas "Dr. Raúl Alfonsín" (sede Chascomús). Universidad Nacional de San Martín. Instituto de Investigaciones Biotecnológicas. Instituto de Investigaciones Biotecnológicas "Dr. Raúl Alfonsín" (sede Chascomús); ArgentinaFil: Iglesias, Alberto Alvaro. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Santa Fe. Instituto de Agrobiotecnología del Litoral. Universidad Nacional del Litoral. Instituto de Agrobiotecnología del Litoral; ArgentinaSpringer Heidelberg2003-04info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/93436Gomez Casati, Diego Fabian; Cortassa, Sonia del Carmen; Aon, Miguel A.; Iglesias, Alberto Alvaro; Ultrasensitive behavior in the synthesis of storage polysaccharides in cyanobacteria; Springer Heidelberg; Planta; 216; 6; 4-2003; 969-9750032-09351432-2048CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/url/https://link.springer.com/article/10.1007/s00425-002-0949-4info:eu-repo/semantics/altIdentifier/doi/10.1007/s00425-002-0949-4info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2024-05-08T13:51:32Zoai:ri.conicet.gov.ar:11336/93436instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982024-05-08 13:51:32.988CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
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