Synthesis and Characterization of Photoswitchable Fluorescent Silica Nanoparticles
We have designed and synthesized a new functional (amino reactive)highly efficient fluorescent molecular switch (FMS) with a photochromicdiarylethene and a rhodamine fluorescent dye. The reactive group in thisFMS -N-hydroxysuccinimide ester- allows selective labeling of aminocontaining molecules or...
| Autores: | , , , , , |
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| Tipo de recurso: | artículo |
| Estado: | Versión publicada |
| Fecha de publicación: | 2008 |
| País: | Argentina |
| Institución: | Consejo Nacional de Investigaciones Científicas y Técnicas |
| Repositorio: | CONICET Digital (CONICET) |
| Idioma: | inglés |
| OAI Identifier: | oai:ri.conicet.gov.ar:11336/102995 |
| Acceso en línea: | http://hdl.handle.net/11336/102995 |
| Access Level: | acceso abierto |
| Palabra clave: | colloids fluorescence microscopy molecular switches photochromism https://purl.org/becyt/ford/1.4 https://purl.org/becyt/ford/1 |
| Sumario: | We have designed and synthesized a new functional (amino reactive)highly efficient fluorescent molecular switch (FMS) with a photochromicdiarylethene and a rhodamine fluorescent dye. The reactive group in thisFMS -N-hydroxysuccinimide ester- allows selective labeling of aminocontaining molecules or other materials. In ethanolic solutions, thecompound displays a large fluorescent quantum yield of 52% and alarge fluorescence modulation ratio (94%) between two states that maybe interconverted withred and near-UV light. Silica nanoparticles incorporatingthe new FMS were prepared and characterized, and their spectroscopicand switching properties were also studied. The dye retained itsproperties after the incorporation into the silica, thereby allowing lightinducedreversible high modulation of the fluorescence signal of a singleparticle for up to 60 cycles, before undergoing irreversible photobleaching.Some applications of these particles in fluorescence microscopy arealso demonstrated. In particular, subdiffraction images of nanoparticleswere obtained, in the focal plane of a confocal microscope. |
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