Differential expression of alkaline and neutral invertases in response to environmental stresses: Characterization of an alkaline isoform as a stress-response enzyme in wheat leaves

It is well accepted that sucrose (Suc) metabolism is involved in responses to environmental stresses in many plant species. In the present study we showed that alkaline invertase (A-Inv) expression is up-regulated in wheat leaves after an osmotic stress or a low-temperature treatment. We demonstrate...

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Autores: Vargas, Walter Alberto, Pontis, Horacio Guillermo, Salerno, Graciela Lidia
Tipo de recurso: artículo
Estado:Versión publicada
Fecha de publicación:2007
País:Argentina
Institución:Consejo Nacional de Investigaciones Científicas y Técnicas
Repositorio:CONICET Digital (CONICET)
Idioma:inglés
OAI Identifier:oai:ri.conicet.gov.ar:11336/131613
Acceso en línea:http://hdl.handle.net/11336/131613
Access Level:acceso abierto
Palabra clave:ALKALINE INVERTASE
COLD STRESS
OSMOTIC STRESS
SUCROSE HYDROLYSIS
TRANSCRIPTIONAL REGULATION
WHEAT
https://purl.org/becyt/ford/1.6
https://purl.org/becyt/ford/1
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repository_id_str
spelling Differential expression of alkaline and neutral invertases in response to environmental stresses: Characterization of an alkaline isoform as a stress-response enzyme in wheat leavesVargas, Walter AlbertoPontis, Horacio GuillermoSalerno, Graciela LidiaALKALINE INVERTASECOLD STRESSOSMOTIC STRESSSUCROSE HYDROLYSISTRANSCRIPTIONAL REGULATIONWHEAThttps://purl.org/becyt/ford/1.6https://purl.org/becyt/ford/1It is well accepted that sucrose (Suc) metabolism is involved in responses to environmental stresses in many plant species. In the present study we showed that alkaline invertase (A-Inv) expression is up-regulated in wheat leaves after an osmotic stress or a low-temperature treatment. We demonstrated that the increase of total alkaline/neutral Inv activity in wheat leaves after a stress could be due to the induction of an A-Inv isoform. Also, we identified and functionally characterized the first wheat cDNA sequence that codes for an A-Inv. The wheat leaf full-length sequence encoded a protein 70% similar to a neutral Inv of Lolium temulentum; however, after functional characterization, it resulted to encode a protein that hydrolyzed Suc to hexoses with an optimum pH of 8, and, consequently, the encoding sequence was named Ta-A-Inv. By RT-PCR assays we demonstrated that Ta-A-Inv expression is induced in response to osmotic and cold stress in mature primary wheat leaves. We propose that Ta-A-Inv activity could play an important role associated with a more efficient cytosolic Suc hydrolysis during environmental stresses. © 2007 Springer-Verlag.Fil: Vargas, Walter Alberto. Fundación para Investigaciones Biológicas Aplicadas; Argentina. Texas A&M University; Estados Unidos. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Mar del Plata; ArgentinaFil: Pontis, Horacio Guillermo. Fundación para Investigaciones Biológicas Aplicadas; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Mar del Plata; ArgentinaFil: Salerno, Graciela Lidia. Fundación para Investigaciones Biológicas Aplicadas; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Mar del Plata; ArgentinaSpringer2007-11info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/131613Vargas, Walter Alberto; Pontis, Horacio Guillermo; Salerno, Graciela Lidia; Differential expression of alkaline and neutral invertases in response to environmental stresses: Characterization of an alkaline isoform as a stress-response enzyme in wheat leaves; Springer; Planta; 226; 6; 11-2007; 1535-15450032-0935CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/url/https://link.springer.com/article/10.1007/s00425-007-0590-3info:eu-repo/semantics/altIdentifier/doi/10.1007/s00425-007-0590-3info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2024-05-08T13:41:24Zoai:ri.conicet.gov.ar:11336/131613instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982024-05-08 13:41:24.384CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Differential expression of alkaline and neutral invertases in response to environmental stresses: Characterization of an alkaline isoform as a stress-response enzyme in wheat leaves
title Differential expression of alkaline and neutral invertases in response to environmental stresses: Characterization of an alkaline isoform as a stress-response enzyme in wheat leaves
spellingShingle Differential expression of alkaline and neutral invertases in response to environmental stresses: Characterization of an alkaline isoform as a stress-response enzyme in wheat leaves
Vargas, Walter Alberto
ALKALINE INVERTASE
COLD STRESS
OSMOTIC STRESS
SUCROSE HYDROLYSIS
TRANSCRIPTIONAL REGULATION
WHEAT
https://purl.org/becyt/ford/1.6
https://purl.org/becyt/ford/1
title_short Differential expression of alkaline and neutral invertases in response to environmental stresses: Characterization of an alkaline isoform as a stress-response enzyme in wheat leaves
title_full Differential expression of alkaline and neutral invertases in response to environmental stresses: Characterization of an alkaline isoform as a stress-response enzyme in wheat leaves
title_fullStr Differential expression of alkaline and neutral invertases in response to environmental stresses: Characterization of an alkaline isoform as a stress-response enzyme in wheat leaves
title_full_unstemmed Differential expression of alkaline and neutral invertases in response to environmental stresses: Characterization of an alkaline isoform as a stress-response enzyme in wheat leaves
title_sort Differential expression of alkaline and neutral invertases in response to environmental stresses: Characterization of an alkaline isoform as a stress-response enzyme in wheat leaves
dc.creator.none.fl_str_mv Vargas, Walter Alberto
Pontis, Horacio Guillermo
Salerno, Graciela Lidia
author Vargas, Walter Alberto
author_facet Vargas, Walter Alberto
Pontis, Horacio Guillermo
Salerno, Graciela Lidia
author_role author
author2 Pontis, Horacio Guillermo
Salerno, Graciela Lidia
author2_role author
author
dc.subject.none.fl_str_mv ALKALINE INVERTASE
COLD STRESS
OSMOTIC STRESS
SUCROSE HYDROLYSIS
TRANSCRIPTIONAL REGULATION
WHEAT
https://purl.org/becyt/ford/1.6
https://purl.org/becyt/ford/1
topic ALKALINE INVERTASE
COLD STRESS
OSMOTIC STRESS
SUCROSE HYDROLYSIS
TRANSCRIPTIONAL REGULATION
WHEAT
https://purl.org/becyt/ford/1.6
https://purl.org/becyt/ford/1
description It is well accepted that sucrose (Suc) metabolism is involved in responses to environmental stresses in many plant species. In the present study we showed that alkaline invertase (A-Inv) expression is up-regulated in wheat leaves after an osmotic stress or a low-temperature treatment. We demonstrated that the increase of total alkaline/neutral Inv activity in wheat leaves after a stress could be due to the induction of an A-Inv isoform. Also, we identified and functionally characterized the first wheat cDNA sequence that codes for an A-Inv. The wheat leaf full-length sequence encoded a protein 70% similar to a neutral Inv of Lolium temulentum; however, after functional characterization, it resulted to encode a protein that hydrolyzed Suc to hexoses with an optimum pH of 8, and, consequently, the encoding sequence was named Ta-A-Inv. By RT-PCR assays we demonstrated that Ta-A-Inv expression is induced in response to osmotic and cold stress in mature primary wheat leaves. We propose that Ta-A-Inv activity could play an important role associated with a more efficient cytosolic Suc hydrolysis during environmental stresses. © 2007 Springer-Verlag.
publishDate 2007
dc.date.none.fl_str_mv 2007-11
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/131613
Vargas, Walter Alberto; Pontis, Horacio Guillermo; Salerno, Graciela Lidia; Differential expression of alkaline and neutral invertases in response to environmental stresses: Characterization of an alkaline isoform as a stress-response enzyme in wheat leaves; Springer; Planta; 226; 6; 11-2007; 1535-1545
0032-0935
CONICET Digital
CONICET
url http://hdl.handle.net/11336/131613
identifier_str_mv Vargas, Walter Alberto; Pontis, Horacio Guillermo; Salerno, Graciela Lidia; Differential expression of alkaline and neutral invertases in response to environmental stresses: Characterization of an alkaline isoform as a stress-response enzyme in wheat leaves; Springer; Planta; 226; 6; 11-2007; 1535-1545
0032-0935
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/url/https://link.springer.com/article/10.1007/s00425-007-0590-3
info:eu-repo/semantics/altIdentifier/doi/10.1007/s00425-007-0590-3
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
application/pdf
application/pdf
dc.publisher.none.fl_str_mv Springer
publisher.none.fl_str_mv Springer
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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score 15.811543

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