Cholinergic autoantibodies from primary Sjögren’s syndrome inhibit mucin production via phospholipase c and cyclooxygenase-2 in the rat submandibular gland

BACKGROUND: Patients with primary Sjögren's syndrome (pSS) produce functional IgG against cholinoreceptor of exocrine glands modifying their activity. The aim of the present work was to demonstrate pSS IgG antibodies (pSS IgG) interacting with M(3) muscarinic acetylcholine receptors (mAChR) of...

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Autores: Passafaro, Daniela, Sterin, Leonor Josefina, Reina, Silvia Lorena, Borda, Enri Santiago
Formato: artículo
Estado:Versión publicada
Fecha de publicación:2011
País:Argentina
Recursos:Consejo Nacional de Investigaciones Científicas y Técnicas
Repositorio:CONICET Digital (CONICET)
Idioma:inglés
OAI Identifier:oai:ri.conicet.gov.ar:11336/16419
Acesso em linha:http://hdl.handle.net/11336/16419
Access Level:acceso abierto
Palavra-chave:PRIMARY SJÖGREN SYNDROME
MUCIN
SUBMANDIBULAR GLAND
PGE2
AUTO ANTIBODIES
InsP
mAChR
https://purl.org/becyt/ford/3.3
https://purl.org/becyt/ford/3
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oai_identifier_str oai:ri.conicet.gov.ar:11336/16419
network_acronym_str AR
network_name_str Argentina
repository_id_str
spelling Cholinergic autoantibodies from primary Sjögren’s syndrome inhibit mucin production via phospholipase c and cyclooxygenase-2 in the rat submandibular glandPassafaro, DanielaSterin, Leonor JosefinaReina, Silvia LorenaBorda, Enri SantiagoPRIMARY SJÖGREN SYNDROMEMUCINSUBMANDIBULAR GLANDPGE2AUTO ANTIBODIESInsPmAChRhttps://purl.org/becyt/ford/3.3https://purl.org/becyt/ford/3BACKGROUND: Patients with primary Sjögren's syndrome (pSS) produce functional IgG against cholinoreceptor of exocrine glands modifying their activity. The aim of the present work was to demonstrate pSS IgG antibodies (pSS IgG) interacting with M(3) muscarinic acetylcholine receptors (mAChR) of rats submandibular glands that alter mucin release and production via phospholipase C (PLC) and cyclooxigenase-2 (COX-2) pathways. METHODS: Mucin release and production of prostaglandin E2 (PGE2), and total inositol phosphates (InsP) were measured in rat submandibular gland in the presence of pSS IgG auto antibodies. RESULTS: The auto antibodies interacting with M3 mAChR decreased mucin release and production through stimulation of PLC and COX-2. This stimulation leads to an incremental increase in InsP production and in PGE2 generation, inducing signalling through the prostaglandin membrane receptors subtype 2 (EP2). Moreover, the decrease in mucin production had negative correlation with PGE(2) generation and InsP accumulation. CONCLUSION: IgG in patients with pSS could play an important role in the pathoetiology of dry mouth, decreasing the salivary mucin through the production of proinflammatory substances and leading to the reduction in the protection of the oral tissues.Fil: Passafaro, Daniela. Universidad de Buenos Aires. Facultad de Odontología. Cátedra de Farmacología; ArgentinaFil: Sterin, Leonor Josefina. Universidad de Buenos Aires. Facultad de Odontología. Cátedra de Farmacología; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaFil: Reina, Silvia Lorena. Universidad de Buenos Aires. Facultad de Odontología. Cátedra de Farmacología; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaFil: Borda, Enri Santiago. Universidad de Buenos Aires. Facultad de Odontología. Cátedra de Farmacología; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaIsfahan Univ Med Sciences2011-03info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/16419Passafaro, Daniela; Sterin, Leonor Josefina; Reina, Silvia Lorena; Borda, Enri Santiago; Cholinergic autoantibodies from primary Sjögren’s syndrome inhibit mucin production via phospholipase c and cyclooxygenase-2 in the rat submandibular gland; Isfahan Univ Med Sciences; Dental Research Journal; 8; 3; 3-2011; 138-1451735-33272008-0255enginfo:eu-repo/semantics/altIdentifier/url/http://drj.mui.ac.ir/index.php/drj/article/view/245info:eu-repo/semantics/altIdentifier/url/https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3177389/info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2024-05-08T13:56:57Zoai:ri.conicet.gov.ar:11336/16419instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982024-05-08 13:56:58.263CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Cholinergic autoantibodies from primary Sjögren’s syndrome inhibit mucin production via phospholipase c and cyclooxygenase-2 in the rat submandibular gland
title Cholinergic autoantibodies from primary Sjögren’s syndrome inhibit mucin production via phospholipase c and cyclooxygenase-2 in the rat submandibular gland
spellingShingle Cholinergic autoantibodies from primary Sjögren’s syndrome inhibit mucin production via phospholipase c and cyclooxygenase-2 in the rat submandibular gland
Passafaro, Daniela
PRIMARY SJÖGREN SYNDROME
MUCIN
SUBMANDIBULAR GLAND
PGE2
AUTO ANTIBODIES
InsP
mAChR
https://purl.org/becyt/ford/3.3
https://purl.org/becyt/ford/3
title_short Cholinergic autoantibodies from primary Sjögren’s syndrome inhibit mucin production via phospholipase c and cyclooxygenase-2 in the rat submandibular gland
title_full Cholinergic autoantibodies from primary Sjögren’s syndrome inhibit mucin production via phospholipase c and cyclooxygenase-2 in the rat submandibular gland
title_fullStr Cholinergic autoantibodies from primary Sjögren’s syndrome inhibit mucin production via phospholipase c and cyclooxygenase-2 in the rat submandibular gland
title_full_unstemmed Cholinergic autoantibodies from primary Sjögren’s syndrome inhibit mucin production via phospholipase c and cyclooxygenase-2 in the rat submandibular gland
title_sort Cholinergic autoantibodies from primary Sjögren’s syndrome inhibit mucin production via phospholipase c and cyclooxygenase-2 in the rat submandibular gland
dc.creator.none.fl_str_mv Passafaro, Daniela
Sterin, Leonor Josefina
Reina, Silvia Lorena
Borda, Enri Santiago
author Passafaro, Daniela
author_facet Passafaro, Daniela
Sterin, Leonor Josefina
Reina, Silvia Lorena
Borda, Enri Santiago
author_role author
author2 Sterin, Leonor Josefina
Reina, Silvia Lorena
Borda, Enri Santiago
author2_role author
author
author
dc.subject.none.fl_str_mv PRIMARY SJÖGREN SYNDROME
MUCIN
SUBMANDIBULAR GLAND
PGE2
AUTO ANTIBODIES
InsP
mAChR
https://purl.org/becyt/ford/3.3
https://purl.org/becyt/ford/3
topic PRIMARY SJÖGREN SYNDROME
MUCIN
SUBMANDIBULAR GLAND
PGE2
AUTO ANTIBODIES
InsP
mAChR
https://purl.org/becyt/ford/3.3
https://purl.org/becyt/ford/3
description BACKGROUND: Patients with primary Sjögren's syndrome (pSS) produce functional IgG against cholinoreceptor of exocrine glands modifying their activity. The aim of the present work was to demonstrate pSS IgG antibodies (pSS IgG) interacting with M(3) muscarinic acetylcholine receptors (mAChR) of rats submandibular glands that alter mucin release and production via phospholipase C (PLC) and cyclooxigenase-2 (COX-2) pathways. METHODS: Mucin release and production of prostaglandin E2 (PGE2), and total inositol phosphates (InsP) were measured in rat submandibular gland in the presence of pSS IgG auto antibodies. RESULTS: The auto antibodies interacting with M3 mAChR decreased mucin release and production through stimulation of PLC and COX-2. This stimulation leads to an incremental increase in InsP production and in PGE2 generation, inducing signalling through the prostaglandin membrane receptors subtype 2 (EP2). Moreover, the decrease in mucin production had negative correlation with PGE(2) generation and InsP accumulation. CONCLUSION: IgG in patients with pSS could play an important role in the pathoetiology of dry mouth, decreasing the salivary mucin through the production of proinflammatory substances and leading to the reduction in the protection of the oral tissues.
publishDate 2011
dc.date.none.fl_str_mv 2011-03
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/16419
Passafaro, Daniela; Sterin, Leonor Josefina; Reina, Silvia Lorena; Borda, Enri Santiago; Cholinergic autoantibodies from primary Sjögren’s syndrome inhibit mucin production via phospholipase c and cyclooxygenase-2 in the rat submandibular gland; Isfahan Univ Med Sciences; Dental Research Journal; 8; 3; 3-2011; 138-145
1735-3327
2008-0255
url http://hdl.handle.net/11336/16419
identifier_str_mv Passafaro, Daniela; Sterin, Leonor Josefina; Reina, Silvia Lorena; Borda, Enri Santiago; Cholinergic autoantibodies from primary Sjögren’s syndrome inhibit mucin production via phospholipase c and cyclooxygenase-2 in the rat submandibular gland; Isfahan Univ Med Sciences; Dental Research Journal; 8; 3; 3-2011; 138-145
1735-3327
2008-0255
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/url/http://drj.mui.ac.ir/index.php/drj/article/view/245
info:eu-repo/semantics/altIdentifier/url/https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3177389/
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
dc.publisher.none.fl_str_mv Isfahan Univ Med Sciences
publisher.none.fl_str_mv Isfahan Univ Med Sciences
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
_version_ 1799195579870347264
score 15.811543

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