Rabbit IgG antibodies against phospholipase A2 from Crotalus durissus terrificus neutralize the lethal activity of the venom
Crotalus durissus terrificus (C.d.t.) (South American rattlesnake) venom possesses myotoxic and neurotoxic activities, both of which are also expressed by crotoxin, the principal toxin of this venom. Crotoxin contains a basic phospholipase A2 (PLA2) and a non toxic acidic protein, crotapotin. We hav...
| Autores: | , , , , , |
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| Tipo de documento: | artigo |
| Estado: | Versão publicada |
| Data de publicação: | 2006 |
| País: | Argentina |
| Recursos: | Universidad Nacional de Buenos Aires. Facultad de Ciencias Exactas y Naturales |
| Repositório: | Biblioteca Digital (UBA-FCEN) |
| Idioma: | inglês |
| OAI Identifier: | paperaa:paper_00257680_v66_n6_p512_Rodriguez |
| Acesso em linha: | http://hdl.handle.net/20.500.12110/paper_00257680_v66_n6_p512_Rodriguez |
| Access Level: | Acceso aberto |
| Palavra-chave: | Antibodies IgG Crotalus durissus terrificus Neutralization Phospholipase A2 crotoxin Freund adjuvant immunoglobulin G phospholipase A2 snake venom snake venom antiserum animal experiment animal model article controlled study drug efficacy envenomation enzyme isolation gel filtration chromatography incubation time inoculation lethality male mortality mouse muscle disease neurotoxicity nonhuman rabbit snake treatment outcome Animals Antibody Specificity Antivenins Buffers Crotalus Crotoxin Hemolysis Immunoglobulin G Male Mice Neuromuscular Blockade Neutralization Tests Phospholipases A Rabbits |
| Resumo: | Crotalus durissus terrificus (C.d.t.) (South American rattlesnake) venom possesses myotoxic and neurotoxic activities, both of which are also expressed by crotoxin, the principal toxin of this venom. Crotoxin contains a basic phospholipase A2 (PLA2) and a non toxic acidic protein, crotapotin. We have produced and investigated the ability of IgG antibodies raised in rabbits against PLA2 to neutralize the lethality of the whole venom. PLA2 was isolated by gel filtration chromatography (Sephadex G-75). Specific antibodies were obtained by subcutaneous and intramuscular inoculation of PLA2 (700 μg) with Freund adjuvant. Groups of six mice (20 ± 2 g) were inoculated with 0.5 ml i.p. of C. d. t. venom (4 μg) or a mixture of venom that had been preincubated with the desired volume of IgG antibodies. Mortality, recorded 24 and 48 h after inoculation, showed that IgG anti-PLA2 were more effective than anticrotalic serum in neutralizing the lethal activity. These results demonstrate that it could be possible to obtain an anti-venom made by specific antibodies with a high level of protection against the lethal component of C.d.t. venom, and/or the inclusion of these antibodies as a supplement in heterologous anti-venoms. |
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