Cytochemical localization of acid phosphatase in Stigeoclonium tenue (Chaetophorales, Chlorophyceae)

Nonspecific acid phosphatases are a group of enzymes whose activity increases the availability of exogenous and endogenous orthophosphate either through extra- or intracellular hydrolysis of phosphate compounds. Our study demonstrates the activity of acid phosphatases in the filamentous freshwater a...

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Detalles Bibliográficos
Autores: Michetti, Karina Mariel, Leonardi, Patricia Ines, Caceres, Eduardo Jorge
Tipo de recurso: artículo
Estado:Versión publicada
Fecha de publicación:2006
País:Argentina
Institución:Consejo Nacional de Investigaciones Científicas y Técnicas
Repositorio:CONICET Digital (CONICET)
Idioma:inglés
OAI Identifier:oai:ri.conicet.gov.ar:11336/34863
Acceso en línea:http://hdl.handle.net/11336/34863
Access Level:acceso abierto
Palabra clave:CHAETOPHORALES
ACID PHOSPHATASE
STIGEOCLONIUM TENUE
ULTRASTRUCTURE
https://purl.org/becyt/ford/1.6
https://purl.org/becyt/ford/1
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spelling Cytochemical localization of acid phosphatase in Stigeoclonium tenue (Chaetophorales, Chlorophyceae)Michetti, Karina MarielLeonardi, Patricia InesCaceres, Eduardo JorgeCHAETOPHORALESACID PHOSPHATASESTIGEOCLONIUM TENUEULTRASTRUCTUREhttps://purl.org/becyt/ford/1.6https://purl.org/becyt/ford/1Nonspecific acid phosphatases are a group of enzymes whose activity increases the availability of exogenous and endogenous orthophosphate either through extra- or intracellular hydrolysis of phosphate compounds. Our study demonstrates the activity of acid phosphatases in the filamentous freshwater alga Stigeoclonium tenue. These enzymes were detected following a cerium-based method in which cerium was used as an orthophosphate-capture reagent. In thalli from S. tenue from the natural environment, acid phosphatases were found in the longitudinal cell wall, plasmalemma, and vacuole. In thalli from Bold’s Basal Medium culture, these enzymes were found mainly in the plasmalemma; they were scarce in the cell wall. In the thalli grown in phosphate-enriched culture medium, enzymes were found only in the plasmalemma. The low availability of orthophosphate in the medium seems to induce the transport of these enzymes to the cell wall. Its abundance, on the contrary, seems to attenuate this response without affecting the localization of acid phosphatases in the plasmalemma.Fil: Michetti, Karina Mariel. Universidad Nacional del Sur. Departamento de Biología, Bioquímica y Farmacia. Laboratorio de Ficología y Micología; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaFil: Leonardi, Patricia Ines. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Bahía Blanca. Centro de Recursos Naturales Renovables de la Zona Semiárida. Universidad Nacional del Sur. Centro de Recursos Naturales Renovables de la Zona Semiárida; Argentina. Universidad Nacional del Sur. Departamento de Biología, Bioquímica y Farmacia; ArgentinaFil: Caceres, Eduardo Jorge. Universidad Nacional del Sur. Departamento de Biología, Bioquímica y Farmacia. Laboratorio de Ficología y Micología; Argentina. Provincia de Buenos Aires. Gobernación. Comisión de Investigaciones Científicas; ArgentinaInstituto de Histología y Embriología2006-12info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/34863Michetti, Karina Mariel; Leonardi, Patricia Ines; Caceres, Eduardo Jorge; Cytochemical localization of acid phosphatase in Stigeoclonium tenue (Chaetophorales, Chlorophyceae); Instituto de Histología y Embriología; Biocell; 30; 3; 12-2006; 491-4960327-9545CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/url/http://www.cricyt.edu.ar/biocell/vol/pdf/30_3/09.pdfinfo:eu-repo/semantics/altIdentifier/url/http://ref.scielo.org/kmh4z9info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2024-05-08T14:07:50Zoai:ri.conicet.gov.ar:11336/34863instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982024-05-08 14:07:51.052CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Cytochemical localization of acid phosphatase in Stigeoclonium tenue (Chaetophorales, Chlorophyceae)
title Cytochemical localization of acid phosphatase in Stigeoclonium tenue (Chaetophorales, Chlorophyceae)
spellingShingle Cytochemical localization of acid phosphatase in Stigeoclonium tenue (Chaetophorales, Chlorophyceae)
Michetti, Karina Mariel
CHAETOPHORALES
ACID PHOSPHATASE
STIGEOCLONIUM TENUE
ULTRASTRUCTURE
https://purl.org/becyt/ford/1.6
https://purl.org/becyt/ford/1
title_short Cytochemical localization of acid phosphatase in Stigeoclonium tenue (Chaetophorales, Chlorophyceae)
title_full Cytochemical localization of acid phosphatase in Stigeoclonium tenue (Chaetophorales, Chlorophyceae)
title_fullStr Cytochemical localization of acid phosphatase in Stigeoclonium tenue (Chaetophorales, Chlorophyceae)
title_full_unstemmed Cytochemical localization of acid phosphatase in Stigeoclonium tenue (Chaetophorales, Chlorophyceae)
title_sort Cytochemical localization of acid phosphatase in Stigeoclonium tenue (Chaetophorales, Chlorophyceae)
dc.creator.none.fl_str_mv Michetti, Karina Mariel
Leonardi, Patricia Ines
Caceres, Eduardo Jorge
author Michetti, Karina Mariel
author_facet Michetti, Karina Mariel
Leonardi, Patricia Ines
Caceres, Eduardo Jorge
author_role author
author2 Leonardi, Patricia Ines
Caceres, Eduardo Jorge
author2_role author
author
dc.subject.none.fl_str_mv CHAETOPHORALES
ACID PHOSPHATASE
STIGEOCLONIUM TENUE
ULTRASTRUCTURE
https://purl.org/becyt/ford/1.6
https://purl.org/becyt/ford/1
topic CHAETOPHORALES
ACID PHOSPHATASE
STIGEOCLONIUM TENUE
ULTRASTRUCTURE
https://purl.org/becyt/ford/1.6
https://purl.org/becyt/ford/1
description Nonspecific acid phosphatases are a group of enzymes whose activity increases the availability of exogenous and endogenous orthophosphate either through extra- or intracellular hydrolysis of phosphate compounds. Our study demonstrates the activity of acid phosphatases in the filamentous freshwater alga Stigeoclonium tenue. These enzymes were detected following a cerium-based method in which cerium was used as an orthophosphate-capture reagent. In thalli from S. tenue from the natural environment, acid phosphatases were found in the longitudinal cell wall, plasmalemma, and vacuole. In thalli from Bold’s Basal Medium culture, these enzymes were found mainly in the plasmalemma; they were scarce in the cell wall. In the thalli grown in phosphate-enriched culture medium, enzymes were found only in the plasmalemma. The low availability of orthophosphate in the medium seems to induce the transport of these enzymes to the cell wall. Its abundance, on the contrary, seems to attenuate this response without affecting the localization of acid phosphatases in the plasmalemma.
publishDate 2006
dc.date.none.fl_str_mv 2006-12
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/34863
Michetti, Karina Mariel; Leonardi, Patricia Ines; Caceres, Eduardo Jorge; Cytochemical localization of acid phosphatase in Stigeoclonium tenue (Chaetophorales, Chlorophyceae); Instituto de Histología y Embriología; Biocell; 30; 3; 12-2006; 491-496
0327-9545
CONICET Digital
CONICET
url http://hdl.handle.net/11336/34863
identifier_str_mv Michetti, Karina Mariel; Leonardi, Patricia Ines; Caceres, Eduardo Jorge; Cytochemical localization of acid phosphatase in Stigeoclonium tenue (Chaetophorales, Chlorophyceae); Instituto de Histología y Embriología; Biocell; 30; 3; 12-2006; 491-496
0327-9545
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/url/http://www.cricyt.edu.ar/biocell/vol/pdf/30_3/09.pdf
info:eu-repo/semantics/altIdentifier/url/http://ref.scielo.org/kmh4z9
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
application/pdf
application/pdf
dc.publisher.none.fl_str_mv Instituto de Histología y Embriología
publisher.none.fl_str_mv Instituto de Histología y Embriología
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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