SRAP as an Informative Molecular Marker to Study the Fusarium poae Genetic Variability
<em>Fusarium poae</em>is one of the<em>Fusarium</em>species isolated from grains associated with Fusarium head blight (FHB), whose occurrence has increased in the last years. In this study, a total of 105<em>F.poae</em>isolates from Argentina, Belgium, Canada, Eng...
| Autores: | , , |
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| Tipo de recurso: | artículo |
| Estado: | Versión enviada para evaluación y publicación |
| Fecha de publicación: | 2015 |
| País: | Argentina |
| Institución: | Comisión de Investigaciones Científicas de la Provincia de Buenos Aires |
| Repositorio: | CIC Digital (CICBA) |
| Idioma: | inglés |
| OAI Identifier: | oai:digital.cic.gba.gob.ar:11746/6292 |
| Acceso en línea: | https://digital.cic.gba.gob.ar/handle/11746/6292 |
| Access Level: | acceso abierto |
| Palabra clave: | Agronomía, reproducción y protección de plantas Fusarium poae genetic variability open reading frames sequence-related amplified polymorphism |
| Sumario: | <em>Fusarium poae</em>is one of the<em>Fusarium</em>species isolated from grains associated with Fusarium head blight (FHB), whose occurrence has increased in the last years. In this study, a total of 105<em>F.poae</em>isolates from Argentina, Belgium, Canada, England, Finland, France, Germany, Hungary, Italy, Luxembourg, Poland, Switzerland and Uruguay were evaluated using sequence-related amplified polymorphism (SRAP) to analyse the capacity of this molecular marker to evaluate the<em>F.poae</em>genetic variability. The molecular analysis showed high intraspecific variability within<em>F.poae</em>isolates, and a partial relationship was revealed between variability and the host/geographic origin. Analysis of molecular variance (amova) indicated a high genetic variability in the<em>F.poae</em>collection, with most of the genetic variability resulting from differences within, rather than between American and European populations. The analysis of sequenced SRAP fragments targets into hypothetical proteins from different<em>Fusarium</em>species showing that the SRAP technique not only allows studying<em>F.poae</em>genetic variability, but also targets coding regions into the<em>F.poae</em>genome. To our knowledge, this is the first report on genetic variability of<em>F.poae</em>using SRAP technique and also demonstrates the efficacy of this molecular marker to amplify open reading frames in fungus. |
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