Comparison of ebony gene from three ebony mutants

Drosophila melanogaster ebony mutant is easily recognizable by its black pigmentation instead of the normal brown color of the wild type strains. This mutant is defective for the synthesis of β-alanyl derivatives such as N-β-alanyldopamine (NBAD) or N-β-alanylhistamine (carcinine), which are the pro...

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Autores: Pérez, Martín Mariano, Rossi, Fabiana Alejandra, Quesada Allue, Luis Alberto
Tipo de recurso: artículo
Estado:Versión publicada
Fecha de publicación:2014
País:Argentina
Institución:Consejo Nacional de Investigaciones Científicas y Técnicas
Repositorio:CONICET Digital (CONICET)
Idioma:inglés
OAI Identifier:oai:ri.conicet.gov.ar:11336/8481
Acceso en línea:http://hdl.handle.net/11336/8481
Access Level:acceso abierto
Palabra clave:Ebony
Nbad-Synthase
Coding Sequence
Alleles
https://purl.org/becyt/ford/1.6
https://purl.org/becyt/ford/1
id AR_7f63ffc2ed5e3637907a06ed4f0d3db3
oai_identifier_str oai:ri.conicet.gov.ar:11336/8481
network_acronym_str AR
network_name_str Argentina
repository_id_str
dc.title.none.fl_str_mv Comparison of ebony gene from three ebony mutants
title Comparison of ebony gene from three ebony mutants
spellingShingle Comparison of ebony gene from three ebony mutants
Pérez, Martín Mariano
Ebony
Nbad-Synthase
Coding Sequence
Alleles
https://purl.org/becyt/ford/1.6
https://purl.org/becyt/ford/1
title_short Comparison of ebony gene from three ebony mutants
title_full Comparison of ebony gene from three ebony mutants
title_fullStr Comparison of ebony gene from three ebony mutants
title_full_unstemmed Comparison of ebony gene from three ebony mutants
title_sort Comparison of ebony gene from three ebony mutants
dc.creator.none.fl_str_mv Pérez, Martín Mariano
Rossi, Fabiana Alejandra
Quesada Allue, Luis Alberto
author Pérez, Martín Mariano
author_facet Pérez, Martín Mariano
Rossi, Fabiana Alejandra
Quesada Allue, Luis Alberto
author_role author
author2 Rossi, Fabiana Alejandra
Quesada Allue, Luis Alberto
author2_role author
author
dc.subject.none.fl_str_mv Ebony
Nbad-Synthase
Coding Sequence
Alleles
https://purl.org/becyt/ford/1.6
https://purl.org/becyt/ford/1
topic Ebony
Nbad-Synthase
Coding Sequence
Alleles
https://purl.org/becyt/ford/1.6
https://purl.org/becyt/ford/1
description Drosophila melanogaster ebony mutant is easily recognizable by its black pigmentation instead of the normal brown color of the wild type strains. This mutant is defective for the synthesis of β-alanyl derivatives such as N-β-alanyldopamine (NBAD) or N-β-alanylhistamine (carcinine), which are the products of the conjugation of β- alanine with dopamine or histamine respectively. Besides body color defect, other wellknown features of this mutant are the neurological and behavioral disorders, such as abnormal electroretinograms, lacking “on” and “off” transients (Hotta and Benzer, 1969), reduced phototaxis (Benzer, 1967) and abnormal circadian rhythm (Newby and Jackson 1991). For many years, Ebony was known as an epidermal enzyme responsible only for tanning and sclerotization of brown cuticles (Wright, 1987). We previously demonstrated that NBAD-synthase is an enzyme induced in epidermis with a narrow window of expression at the beginning of pupariation (Pérez et al., 2002; Wappner et al., 1996a). The enzyme is also induced during the transition from pharate adult to imago and during the first hours after the ecdysis (Pérez et al, 2004: Pérez et al., 2010). Recently, we documented the expression of NBAD-synthase in epidermal tissues of D. melanogaster embryos (Pérez et al., 2010). In addition to the expression of NBAD-synthase in epidermis, we found that this activity is also expressed constitutively in nervous system (Pérez et al., 2004), suggesting a role in the metabolism of neurotransmitters. Furthermore, it has been postulated that this enzyme functions in a metabolic pathway that may terminate the action of histamine in photoreceptor cells (Borycz et al., 2002; Richardt et al., 2003). Our biochemical and immunohistochemical results demonstrated that NBAD-synthase is widely expressed in the brain. Thus the expression of Ebony in brain regions other than retinas suggests that this enzyme not only plays a role in the metabolism of histamine in visual system but also in the metabolism of other neurotransmitters like dopamine and possibly octopamine and serotonin (Pérez et al., 2002; Richardt et al., 2003; Pérez et al, 2004). We previously analyzed NBAD-synthase in vitro activity in the ebony mutants e1 and e4 and we found that they are unable to synthesize β-alanyderivatives (Pérez et al 1997; 2001). We have also cloned and sequenced the e4 mutant gene, showing that it has a 447 base pairs deletion in its first exon, synthesizing a protein without activity due to the lacking of 149 aminoacids (Pérez et al., 2001). Some slight physiological differences exist among the different ebony mutants, with e4 being the less drastic phenotype (Newby and Jackson, 1991; Rossi et al, data not shown; Flybase). To address the reason of this discrepancy we characterized molecularly e1 and e11 to better understand their phenotypes...
publishDate 2014
dc.date.none.fl_str_mv 2014-12
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/8481
Pérez, Martín Mariano; Rossi, Fabiana Alejandra; Quesada Allue, Luis Alberto; Comparison of ebony gene from three ebony mutants; University of Oklahoma; Drosophila Information Service; 97; 12-2014; 30-32
0070-7333
url http://hdl.handle.net/11336/8481
identifier_str_mv Pérez, Martín Mariano; Rossi, Fabiana Alejandra; Quesada Allue, Luis Alberto; Comparison of ebony gene from three ebony mutants; University of Oklahoma; Drosophila Information Service; 97; 12-2014; 30-32
0070-7333
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/url/http://www.ou.edu/journals/dis/DIS97/Rossi%2030.pdf
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
application/pdf
dc.publisher.none.fl_str_mv University of Oklahoma
publisher.none.fl_str_mv University of Oklahoma
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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spelling Comparison of ebony gene from three ebony mutantsPérez, Martín MarianoRossi, Fabiana AlejandraQuesada Allue, Luis AlbertoEbonyNbad-SynthaseCoding SequenceAlleleshttps://purl.org/becyt/ford/1.6https://purl.org/becyt/ford/1Drosophila melanogaster ebony mutant is easily recognizable by its black pigmentation instead of the normal brown color of the wild type strains. This mutant is defective for the synthesis of β-alanyl derivatives such as N-β-alanyldopamine (NBAD) or N-β-alanylhistamine (carcinine), which are the products of the conjugation of β- alanine with dopamine or histamine respectively. Besides body color defect, other wellknown features of this mutant are the neurological and behavioral disorders, such as abnormal electroretinograms, lacking “on” and “off” transients (Hotta and Benzer, 1969), reduced phototaxis (Benzer, 1967) and abnormal circadian rhythm (Newby and Jackson 1991). For many years, Ebony was known as an epidermal enzyme responsible only for tanning and sclerotization of brown cuticles (Wright, 1987). We previously demonstrated that NBAD-synthase is an enzyme induced in epidermis with a narrow window of expression at the beginning of pupariation (Pérez et al., 2002; Wappner et al., 1996a). The enzyme is also induced during the transition from pharate adult to imago and during the first hours after the ecdysis (Pérez et al, 2004: Pérez et al., 2010). Recently, we documented the expression of NBAD-synthase in epidermal tissues of D. melanogaster embryos (Pérez et al., 2010). In addition to the expression of NBAD-synthase in epidermis, we found that this activity is also expressed constitutively in nervous system (Pérez et al., 2004), suggesting a role in the metabolism of neurotransmitters. Furthermore, it has been postulated that this enzyme functions in a metabolic pathway that may terminate the action of histamine in photoreceptor cells (Borycz et al., 2002; Richardt et al., 2003). Our biochemical and immunohistochemical results demonstrated that NBAD-synthase is widely expressed in the brain. Thus the expression of Ebony in brain regions other than retinas suggests that this enzyme not only plays a role in the metabolism of histamine in visual system but also in the metabolism of other neurotransmitters like dopamine and possibly octopamine and serotonin (Pérez et al., 2002; Richardt et al., 2003; Pérez et al, 2004). We previously analyzed NBAD-synthase in vitro activity in the ebony mutants e1 and e4 and we found that they are unable to synthesize β-alanyderivatives (Pérez et al 1997; 2001). We have also cloned and sequenced the e4 mutant gene, showing that it has a 447 base pairs deletion in its first exon, synthesizing a protein without activity due to the lacking of 149 aminoacids (Pérez et al., 2001). Some slight physiological differences exist among the different ebony mutants, with e4 being the less drastic phenotype (Newby and Jackson, 1991; Rossi et al, data not shown; Flybase). To address the reason of this discrepancy we characterized molecularly e1 and e11 to better understand their phenotypes...Fil: Pérez, Martín Mariano. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Parque Centenario. Instituto de Investigaciones Bioquimicas de Buenos Aires; Argentina. Fundación Instituto Leloir; Argentina. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Departamento de Química Biológica; ArgentinaFil: Rossi, Fabiana Alejandra. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Departamento de Química Biológica; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Parque Centenario. Instituto de Investigaciones Bioquimicas de Buenos Aires; Argentina. Fundación Instituto Leloir; ArgentinaFil: Quesada Allue, Luis Alberto. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Parque Centenario. Instituto de Investigaciones Bioquimicas de Buenos Aires; Argentina. Fundación Instituto Leloir; Argentina. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Departamento de Química Biológica; ArgentinaUniversity of Oklahoma2014-12info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/8481Pérez, Martín Mariano; Rossi, Fabiana Alejandra; Quesada Allue, Luis Alberto; Comparison of ebony gene from three ebony mutants; University of Oklahoma; Drosophila Information Service; 97; 12-2014; 30-320070-7333enginfo:eu-repo/semantics/altIdentifier/url/http://www.ou.edu/journals/dis/DIS97/Rossi%2030.pdfinfo:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2024-05-08T13:43:41Zoai:ri.conicet.gov.ar:11336/8481instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982024-05-08 13:43:41.888CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
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