Changes in the surface potential of Lactobacillus acidophilus under freeze-thawing stress
The zeta potential of Lactobacillus acidophilux CRL 640, a measure of the net distribution of electrical charges on the bacterial surface, is a function of the glucose concentration in the growing media. With 2% glucose, cells in the stationary phase showed a zeta potential of -45 ± 2 mV. With these...
| Autores: | , , |
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| Tipo de recurso: | artículo |
| Estado: | Versión publicada |
| Fecha de publicación: | 2000 |
| País: | Argentina |
| Institución: | Consejo Nacional de Investigaciones Científicas y Técnicas |
| Repositorio: | CONICET Digital (CONICET) |
| Idioma: | inglés |
| OAI Identifier: | oai:ri.conicet.gov.ar:11336/51619 |
| Acceso en línea: | http://hdl.handle.net/11336/51619 |
| Access Level: | acceso abierto |
| Palabra clave: | Freeze-Thawing Lactobacillus Acidophilus Surface Potential https://purl.org/becyt/ford/1.4 https://purl.org/becyt/ford/1 |
| Sumario: | The zeta potential of Lactobacillus acidophilux CRL 640, a measure of the net distribution of electrical charges on the bacterial surface, is a function of the glucose concentration in the growing media. With 2% glucose, cells in the stationary phase showed a zeta potential of -45 ± 2 mV. With these cells, the zeta potential after freezing and thawing decreased to -32 ± 2 mV and there was a decrease in viability. The changes in the surface potential correlated with damage to the cell surface as shown by electron microscopy. Freeze-thawed cells incubated in a rich medium recovered a zeta potential of -38 ± 2 mV without cell growth. L. acidophilus CRL 640 showed the same value of surface potential as control cells when they were frozen and thawed in 2 M glycerol. |
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