Application of the knowledge-based approach to strain selection for a bioaugmentation process of phenanthrene- and Cr(VI)-contaminated soil
Aims: The objective of this study was to apply the knowledge-based approach to the selection of an inoculum to be used in bioaugmentation processes to facilitate phenanthrene degradation in phenanthrene- and Cr(VI)-co-contaminated soils. Methods and Results: The bacterial community composition of ph...
| Autores: | , , , , |
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| Tipo de recurso: | artículo |
| Estado: | Versión publicada |
| Fecha de publicación: | 2011 |
| País: | Argentina |
| Institución: | Consejo Nacional de Investigaciones Científicas y Técnicas |
| Repositorio: | CONICET Digital (CONICET) |
| Idioma: | inglés |
| OAI Identifier: | oai:ri.conicet.gov.ar:11336/184144 |
| Acceso en línea: | http://hdl.handle.net/11336/184144 |
| Access Level: | acceso abierto |
| Palabra clave: | BIOAUGMENTATION CR(VI) PHENANTHRENE SPHINGOMONAS SP https://purl.org/becyt/ford/2.8 https://purl.org/becyt/ford/2 |
| Sumario: | Aims: The objective of this study was to apply the knowledge-based approach to the selection of an inoculum to be used in bioaugmentation processes to facilitate phenanthrene degradation in phenanthrene- and Cr(VI)-co-contaminated soils. Methods and Results: The bacterial community composition of phenanthrene and phenanthrene- and Cr(VI)-co-contaminated microcosms, determined by denaturing gradient gel electrophoresis analysis, showed that members of the Sphingomonadaceae family were the predominant micro-organisms. However, the Cr(VI) contamination produced a selective change of predominant Sphingomonas species, and in co-contaminated soil microcosms, a population closely related to Sphingomonas paucimobilis was naturally selected. The bioaugmentation process was carried out using the phenanthrene-degrading strain S. paucimobilis 20006FA, isolated and characterized in our laboratory. Although the strain showed a low Cr(VI) resistance (0·250mmoll-1); in liquid culture, it was capable of reducing chromate and degrading phenanthrene simultaneously. Conclusion: The inoculation of this strain managed to moderate the effect of the presence of Cr(VI), increasing the biological activity and phenanthrene degradation rate in co-contaminated microcosm. Significance and Impact of the Study: In this study, we have applied a novel approach to the selection of the adequate inoculum to enhance the phenanthrene degradation in phenanthrene- and Cr(VI)-co-contaminated soils. © 2011 The Authors. Journal of Applied Microbiology |
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