Enzymatic production of biodiesel from cotton seed oil using t-butanol as a solvent
The enzymatic production of biodiesel by methanolysis of cottonseed oil was studied using immobilized Candida antarctica lipase as catalyst in t-butanol solvent. Methyl ester production and triacylglycerol disappearance were followed by HPLC chromatography. It was found, using a batch system, that e...
| Autores: | , , , |
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| Tipo de recurso: | artículo |
| Estado: | Versión publicada |
| Fecha de publicación: | 2007 |
| País: | Argentina |
| Institución: | Consejo Nacional de Investigaciones Científicas y Técnicas |
| Repositorio: | CONICET Digital (CONICET) |
| Idioma: | inglés |
| OAI Identifier: | oai:ri.conicet.gov.ar:11336/66874 |
| Acceso en línea: | http://hdl.handle.net/11336/66874 |
| Access Level: | acceso abierto |
| Palabra clave: | Biodiesel Cotton Seed Oil Lipase Methanol Novozym 435 T-Butanol https://purl.org/becyt/ford/2.9 https://purl.org/becyt/ford/2 |
| Sumario: | The enzymatic production of biodiesel by methanolysis of cottonseed oil was studied using immobilized Candida antarctica lipase as catalyst in t-butanol solvent. Methyl ester production and triacylglycerol disappearance were followed by HPLC chromatography. It was found, using a batch system, that enzyme inhibition caused by undissolved methanol was eliminated by adding t-butanol to the reaction medium, which also gave a noticeable increase of reaction rate and ester yield. The effect of t-butanol, methanol concentration and temperature on this system was determined. A methanolysis yield of 97% was observed after 24 h at 50 °C with a reaction mixture containing 32.5% t-butanol, 13.5% methanol, 54% oil and 0.017 g enzyme (g oil)-1. With the same mixture, a 95% ester yield was obtained using a one step fixed bed continuous reactor with a flow rate of 9.6 ml h-1 (g enzyme)-1. Experiments with the continuous reactor over 500 h did not show any appreciable decrease in ester yields. © 2006 Elsevier Ltd. All rights reserved. |
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