Levels of milk glycosaminoglycans in Holstein cows with clinical and subclinical mastitis

Mastitis is an inflammation of the mammary gland whose diagnosis is based on clinical criteria and the determination of inflammatory markers. The disease is mainly caused by bacteria causing intra-mammary infections. The etiological agent/s are detected by milk culture or by molecular biology approa...

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Authors: Caggiano, Nicolás, Crespi, Elisa Silvia, Fiorini, Gonzalo, de Simone, Emilio Adrian
Format: article
Status:Published version
Publication Date:2022
Country:Argentina
Institution:Consejo Nacional de Investigaciones Científicas y Técnicas
Repository:CONICET Digital (CONICET)
Language:English
OAI Identifier:oai:ri.conicet.gov.ar:11336/223905
Online Access:http://hdl.handle.net/11336/223905
Access Level:Open access
Keyword:BOVINE MASTITIS
GLYCOSAMINOGLYCANS
MILK
https://purl.org/becyt/ford/4.2
https://purl.org/becyt/ford/4
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oai_identifier_str oai:ri.conicet.gov.ar:11336/223905
network_acronym_str AR
network_name_str Argentina
repository_id_str
spelling Levels of milk glycosaminoglycans in Holstein cows with clinical and subclinical mastitisCaggiano, NicolásCrespi, Elisa SilviaFiorini, Gonzalode Simone, Emilio AdrianBOVINE MASTITISGLYCOSAMINOGLYCANSMILKhttps://purl.org/becyt/ford/4.2https://purl.org/becyt/ford/4Mastitis is an inflammation of the mammary gland whose diagnosis is based on clinical criteria and the determination of inflammatory markers. The disease is mainly caused by bacteria causing intra-mammary infections. The etiological agent/s are detected by milk culture or by molecular biology approaches. However, these methodologies are unpractical for on-farm diagnosis, where rapid decisions must be made when infected animals are detected. For this reason, the levels of glycosaminoglycans (GAGs) in milk were evaluated as a tool for the diagnosis of mastitis. GAGs levels were determined by the dimethylmethylene blue assay (DMMB) method in milk from healthy animals and in milk from animals with mastitis caused by different etiological agents. Low levels of GAGs (1.93 ± 0.40 mg) were found in milk samples obtained from healthy animals (H), while intermediate (2.4 ± 0.61 mg) and high levels (2.87 ± 0.7 mg) were found in milk obtained from cows with subclinical (SM) and clinical mastitis (CM), respectively. Statistically significant differences were found between the SM and H groups (p < 0.001) and between CM and the other groups (p < 0.0001). The highest levels of GAGs were detected in the CM group, in which coagulase negative Staphylococcus (CNS) and Streptococcus spp. were isolated. Animals infected by S. aureus had the highest levels of GAGs in the SM group. The sensitivity (Se) and specificity (Sp) for the assessment of GAGs levels for CM diagnosis were 89.7 % and 81.6 %, respectively. For the diagnosis of SM, Se and Sp were 73.2 % and 95 %, respectively. The area under the curve for the diagnosis of CM was 0.902 (p < 0.0001), while that for SM was 0.833 (p < 0.0001). Results suggest that the measurement of GAGs levels in milk can be used as an indicator of udder health in dairy cattle, since increased levels of GAGs were associated with mastitis.Fil: Caggiano, Nicolás. Universidad de Buenos Aires. Facultad de Ciencias Veterinarias. Cátedra de Fisiología Animal; ArgentinaFil: Crespi, Elisa Silvia. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaFil: Fiorini, Gonzalo. Universidad de Buenos Aires. Facultad de Ciencias Veterinarias. Cátedra de Fisiología Animal; ArgentinaFil: de Simone, Emilio Adrian. Universidad de Buenos Aires. Facultad de Ciencias Veterinarias. Cátedra de Fisiología Animal; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaScientific Technical Research Council Turkey-tubitak2022-03info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/223905Caggiano, Nicolás; Crespi, Elisa Silvia; Fiorini, Gonzalo; de Simone, Emilio Adrian; Levels of milk glycosaminoglycans in Holstein cows with clinical and subclinical mastitis; Scientific Technical Research Council Turkey-tubitak; Turkish Journal Of Veterinary And Animal Sciences; 46; 2; 3-2022; 171-1791300-0128CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/doi/10.55730/1300-0128.4164info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2024-05-08T14:09:09Zoai:ri.conicet.gov.ar:11336/223905instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982024-05-08 14:09:09.523CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Levels of milk glycosaminoglycans in Holstein cows with clinical and subclinical mastitis
title Levels of milk glycosaminoglycans in Holstein cows with clinical and subclinical mastitis
spellingShingle Levels of milk glycosaminoglycans in Holstein cows with clinical and subclinical mastitis
Caggiano, Nicolás
BOVINE MASTITIS
GLYCOSAMINOGLYCANS
MILK
https://purl.org/becyt/ford/4.2
https://purl.org/becyt/ford/4
title_short Levels of milk glycosaminoglycans in Holstein cows with clinical and subclinical mastitis
title_full Levels of milk glycosaminoglycans in Holstein cows with clinical and subclinical mastitis
title_fullStr Levels of milk glycosaminoglycans in Holstein cows with clinical and subclinical mastitis
title_full_unstemmed Levels of milk glycosaminoglycans in Holstein cows with clinical and subclinical mastitis
title_sort Levels of milk glycosaminoglycans in Holstein cows with clinical and subclinical mastitis
dc.creator.none.fl_str_mv Caggiano, Nicolás
Crespi, Elisa Silvia
Fiorini, Gonzalo
de Simone, Emilio Adrian
author Caggiano, Nicolás
author_facet Caggiano, Nicolás
Crespi, Elisa Silvia
Fiorini, Gonzalo
de Simone, Emilio Adrian
author_role author
author2 Crespi, Elisa Silvia
Fiorini, Gonzalo
de Simone, Emilio Adrian
author2_role author
author
author
dc.subject.none.fl_str_mv BOVINE MASTITIS
GLYCOSAMINOGLYCANS
MILK
https://purl.org/becyt/ford/4.2
https://purl.org/becyt/ford/4
topic BOVINE MASTITIS
GLYCOSAMINOGLYCANS
MILK
https://purl.org/becyt/ford/4.2
https://purl.org/becyt/ford/4
description Mastitis is an inflammation of the mammary gland whose diagnosis is based on clinical criteria and the determination of inflammatory markers. The disease is mainly caused by bacteria causing intra-mammary infections. The etiological agent/s are detected by milk culture or by molecular biology approaches. However, these methodologies are unpractical for on-farm diagnosis, where rapid decisions must be made when infected animals are detected. For this reason, the levels of glycosaminoglycans (GAGs) in milk were evaluated as a tool for the diagnosis of mastitis. GAGs levels were determined by the dimethylmethylene blue assay (DMMB) method in milk from healthy animals and in milk from animals with mastitis caused by different etiological agents. Low levels of GAGs (1.93 ± 0.40 mg) were found in milk samples obtained from healthy animals (H), while intermediate (2.4 ± 0.61 mg) and high levels (2.87 ± 0.7 mg) were found in milk obtained from cows with subclinical (SM) and clinical mastitis (CM), respectively. Statistically significant differences were found between the SM and H groups (p < 0.001) and between CM and the other groups (p < 0.0001). The highest levels of GAGs were detected in the CM group, in which coagulase negative Staphylococcus (CNS) and Streptococcus spp. were isolated. Animals infected by S. aureus had the highest levels of GAGs in the SM group. The sensitivity (Se) and specificity (Sp) for the assessment of GAGs levels for CM diagnosis were 89.7 % and 81.6 %, respectively. For the diagnosis of SM, Se and Sp were 73.2 % and 95 %, respectively. The area under the curve for the diagnosis of CM was 0.902 (p < 0.0001), while that for SM was 0.833 (p < 0.0001). Results suggest that the measurement of GAGs levels in milk can be used as an indicator of udder health in dairy cattle, since increased levels of GAGs were associated with mastitis.
publishDate 2022
dc.date.none.fl_str_mv 2022-03
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/223905
Caggiano, Nicolás; Crespi, Elisa Silvia; Fiorini, Gonzalo; de Simone, Emilio Adrian; Levels of milk glycosaminoglycans in Holstein cows with clinical and subclinical mastitis; Scientific Technical Research Council Turkey-tubitak; Turkish Journal Of Veterinary And Animal Sciences; 46; 2; 3-2022; 171-179
1300-0128
CONICET Digital
CONICET
url http://hdl.handle.net/11336/223905
identifier_str_mv Caggiano, Nicolás; Crespi, Elisa Silvia; Fiorini, Gonzalo; de Simone, Emilio Adrian; Levels of milk glycosaminoglycans in Holstein cows with clinical and subclinical mastitis; Scientific Technical Research Council Turkey-tubitak; Turkish Journal Of Veterinary And Animal Sciences; 46; 2; 3-2022; 171-179
1300-0128
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/doi/10.55730/1300-0128.4164
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
dc.publisher.none.fl_str_mv Scientific Technical Research Council Turkey-tubitak
publisher.none.fl_str_mv Scientific Technical Research Council Turkey-tubitak
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
_version_ 1799195945925083136
score 15,811543

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