Development and characterization of miltefosine-loaded polymeric micelles for cancer treatment
Miltefosine presents antineoplastic activity but high hemolytic potential. Its use in cancer has been limited to treating cutaneous metastasis of breast cancer. To decrease hemolytic potential, we developed a formulation of miltefosine-loaded polymeric micelles (PM) of the copolymer Pluronic-F127. A...
| Autores: | , , , , , , , , |
|---|---|
| Tipo de recurso: | artículo |
| Estado: | Versión publicada |
| Fecha de publicación: | 2017 |
| País: | Argentina |
| Institución: | Consejo Nacional de Investigaciones Científicas y Técnicas |
| Repositorio: | CONICET Digital (CONICET) |
| Idioma: | inglés |
| OAI Identifier: | oai:ri.conicet.gov.ar:11336/65640 |
| Acceso en línea: | http://hdl.handle.net/11336/65640 |
| Access Level: | acceso abierto |
| Palabra clave: | Alkylphosphocholines Block-Copolymer Cancer Therapy Hemolytic Potential Miltefosine Polymeric Micelles https://purl.org/becyt/ford/2.10 https://purl.org/becyt/ford/2 |
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Development and characterization of miltefosine-loaded polymeric micelles for cancer treatmentValenzuela Oses, Johanna K.García, Mónica CristinaFeitosa, Valker A.Pachioni Vasconcelos, Juliana A.Gomes-Filho, Sandro M.Lourenço, Felipe R.Cerize, Natalia N.P.Bassères, Daniela S.Rangel-Yagui, Carlota O.AlkylphosphocholinesBlock-CopolymerCancer TherapyHemolytic PotentialMiltefosinePolymeric Micelleshttps://purl.org/becyt/ford/2.10https://purl.org/becyt/ford/2Miltefosine presents antineoplastic activity but high hemolytic potential. Its use in cancer has been limited to treating cutaneous metastasis of breast cancer. To decrease hemolytic potential, we developed a formulation of miltefosine-loaded polymeric micelles (PM) of the copolymer Pluronic-F127. A central composite design was applied and the analysis of variance showed that the optimum level of hydrodynamic diameter and polydispersity index predicted by the model and experimentally confirmed were 29 nm and 0.105, respectively. Thermal analyses confirmed that miltefosine was molecularly dispersed within PM. Pluronic-F127 PM with miltefosine 80 μM presented a significant reduction of hemolytic effect (80%, p < 0.05) in comparison to free drug. In vitro assays against HeLa carcinoma cells demonstrated similar cytotoxicity to free miltefosine and PM. Our results suggest that, by lowering hemolytic potential, miltefosine-loaded Pluronic-F127 PM a promising alternative to broaden this drug use in cancer therapy, as well as of other alkylphosphocholines.Fil: Valenzuela Oses, Johanna K.. Universidade de Sao Paulo; BrasilFil: García, Mónica Cristina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba. Unidad de Investigación y Desarrollo en Tecnología Farmacéutica. Universidad Nacional de Córdoba. Facultad de Ciencias Químicas. Unidad de Investigación y Desarrollo en Tecnología Farmacéutica; ArgentinaFil: Feitosa, Valker A.. Universidade de Sao Paulo; Brasil. Institute for Technological Research; BrasilFil: Pachioni Vasconcelos, Juliana A.. Universidade de Sao Paulo; BrasilFil: Gomes-Filho, Sandro M.. Universidade de Sao Paulo; BrasilFil: Lourenço, Felipe R.. Universidade de Sao Paulo; BrasilFil: Cerize, Natalia N.P.. Institute for Technological Research; BrasilFil: Bassères, Daniela S.. Universidade de Sao Paulo; BrasilFil: Rangel-Yagui, Carlota O.. Universidade de Sao Paulo; BrasilElsevier Science2017-12info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/65640Valenzuela Oses, Johanna K.; García, Mónica Cristina; Feitosa, Valker A.; Pachioni Vasconcelos, Juliana A.; Gomes-Filho, Sandro M.; et al.; Development and characterization of miltefosine-loaded polymeric micelles for cancer treatment; Elsevier Science; Materials Science and Engineering: C; 81; 12-2017; 327-3330928-4931CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/url/http://linkinghub.elsevier.com/retrieve/pii/S0928493117320921info:eu-repo/semantics/altIdentifier/doi/10.1016/j.msec.2017.07.040info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2024-05-08T13:35:18Zoai:ri.conicet.gov.ar:11336/65640instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982024-05-08 13:35:19.035CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse |
| dc.title.none.fl_str_mv |
Development and characterization of miltefosine-loaded polymeric micelles for cancer treatment |
| title |
Development and characterization of miltefosine-loaded polymeric micelles for cancer treatment |
| spellingShingle |
Development and characterization of miltefosine-loaded polymeric micelles for cancer treatment Valenzuela Oses, Johanna K. Alkylphosphocholines Block-Copolymer Cancer Therapy Hemolytic Potential Miltefosine Polymeric Micelles https://purl.org/becyt/ford/2.10 https://purl.org/becyt/ford/2 |
| title_short |
Development and characterization of miltefosine-loaded polymeric micelles for cancer treatment |
| title_full |
Development and characterization of miltefosine-loaded polymeric micelles for cancer treatment |
| title_fullStr |
Development and characterization of miltefosine-loaded polymeric micelles for cancer treatment |
| title_full_unstemmed |
Development and characterization of miltefosine-loaded polymeric micelles for cancer treatment |
| title_sort |
Development and characterization of miltefosine-loaded polymeric micelles for cancer treatment |
| dc.creator.none.fl_str_mv |
Valenzuela Oses, Johanna K. García, Mónica Cristina Feitosa, Valker A. Pachioni Vasconcelos, Juliana A. Gomes-Filho, Sandro M. Lourenço, Felipe R. Cerize, Natalia N.P. Bassères, Daniela S. Rangel-Yagui, Carlota O. |
| author |
Valenzuela Oses, Johanna K. |
| author_facet |
Valenzuela Oses, Johanna K. García, Mónica Cristina Feitosa, Valker A. Pachioni Vasconcelos, Juliana A. Gomes-Filho, Sandro M. Lourenço, Felipe R. Cerize, Natalia N.P. Bassères, Daniela S. Rangel-Yagui, Carlota O. |
| author_role |
author |
| author2 |
García, Mónica Cristina Feitosa, Valker A. Pachioni Vasconcelos, Juliana A. Gomes-Filho, Sandro M. Lourenço, Felipe R. Cerize, Natalia N.P. Bassères, Daniela S. Rangel-Yagui, Carlota O. |
| author2_role |
author author author author author author author author |
| dc.subject.none.fl_str_mv |
Alkylphosphocholines Block-Copolymer Cancer Therapy Hemolytic Potential Miltefosine Polymeric Micelles https://purl.org/becyt/ford/2.10 https://purl.org/becyt/ford/2 |
| topic |
Alkylphosphocholines Block-Copolymer Cancer Therapy Hemolytic Potential Miltefosine Polymeric Micelles https://purl.org/becyt/ford/2.10 https://purl.org/becyt/ford/2 |
| description |
Miltefosine presents antineoplastic activity but high hemolytic potential. Its use in cancer has been limited to treating cutaneous metastasis of breast cancer. To decrease hemolytic potential, we developed a formulation of miltefosine-loaded polymeric micelles (PM) of the copolymer Pluronic-F127. A central composite design was applied and the analysis of variance showed that the optimum level of hydrodynamic diameter and polydispersity index predicted by the model and experimentally confirmed were 29 nm and 0.105, respectively. Thermal analyses confirmed that miltefosine was molecularly dispersed within PM. Pluronic-F127 PM with miltefosine 80 μM presented a significant reduction of hemolytic effect (80%, p < 0.05) in comparison to free drug. In vitro assays against HeLa carcinoma cells demonstrated similar cytotoxicity to free miltefosine and PM. Our results suggest that, by lowering hemolytic potential, miltefosine-loaded Pluronic-F127 PM a promising alternative to broaden this drug use in cancer therapy, as well as of other alkylphosphocholines. |
| publishDate |
2017 |
| dc.date.none.fl_str_mv |
2017-12 |
| dc.type.none.fl_str_mv |
info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion http://purl.org/coar/resource_type/c_6501 info:ar-repo/semantics/articulo |
| format |
article |
| status_str |
publishedVersion |
| dc.identifier.none.fl_str_mv |
http://hdl.handle.net/11336/65640 Valenzuela Oses, Johanna K.; García, Mónica Cristina; Feitosa, Valker A.; Pachioni Vasconcelos, Juliana A.; Gomes-Filho, Sandro M.; et al.; Development and characterization of miltefosine-loaded polymeric micelles for cancer treatment; Elsevier Science; Materials Science and Engineering: C; 81; 12-2017; 327-333 0928-4931 CONICET Digital CONICET |
| url |
http://hdl.handle.net/11336/65640 |
| identifier_str_mv |
Valenzuela Oses, Johanna K.; García, Mónica Cristina; Feitosa, Valker A.; Pachioni Vasconcelos, Juliana A.; Gomes-Filho, Sandro M.; et al.; Development and characterization of miltefosine-loaded polymeric micelles for cancer treatment; Elsevier Science; Materials Science and Engineering: C; 81; 12-2017; 327-333 0928-4931 CONICET Digital CONICET |
| dc.language.none.fl_str_mv |
eng |
| language |
eng |
| dc.relation.none.fl_str_mv |
info:eu-repo/semantics/altIdentifier/url/http://linkinghub.elsevier.com/retrieve/pii/S0928493117320921 info:eu-repo/semantics/altIdentifier/doi/10.1016/j.msec.2017.07.040 |
| dc.rights.none.fl_str_mv |
info:eu-repo/semantics/openAccess https://creativecommons.org/licenses/by-nc-sa/2.5/ar/ |
| eu_rights_str_mv |
openAccess |
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https://creativecommons.org/licenses/by-nc-sa/2.5/ar/ |
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application/pdf application/pdf |
| dc.publisher.none.fl_str_mv |
Elsevier Science |
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Elsevier Science |
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reponame:CONICET Digital (CONICET) instname:Consejo Nacional de Investigaciones Científicas y Técnicas |
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Consejo Nacional de Investigaciones Científicas y Técnicas |
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CONICET Digital (CONICET) |
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CONICET Digital (CONICET) |
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CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas |
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dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar |
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15,812429 |