Increased cholesterol efflux capacity in metabolic syndrome: Relation with qualitative alterations in HDL and LCAT

Background: Metabolic syndrome (MetS) is associated with changes in HDL levels, composition and sub-fraction profile. Whether these alterations affect HDL anti-atherogenic function, specifically measured as its capacity to perform cholesterol efflux, is not yet clearly known. Objective: To evaluate...

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Detalles Bibliográficos
Autores: Lucero, Diego Martín, Svidirov, Denis, Freeman, Lita, López, Graciela I., Fassio, Eduardo, Remaley, Alan T., Schreier, Laura Ester
Tipo de recurso: artículo
Estado:Versión publicada
Fecha de publicación:2015
País:Argentina
Institución:Consejo Nacional de Investigaciones Científicas y Técnicas
Repositorio:CONICET Digital (CONICET)
Idioma:inglés
OAI Identifier:oai:ri.conicet.gov.ar:11336/117684
Acceso en línea:http://hdl.handle.net/11336/117684
Access Level:acceso abierto
Palabra clave:CHOLESTEROL EFFLUX CAPACITY
HDL
LCAT
METABOLIC SYNDROME
PRE-Β1-HDL
https://purl.org/becyt/ford/3.3
https://purl.org/becyt/ford/3
id AR_7d5fb75cee3fbd210d239ea2a3abd905
oai_identifier_str oai:ri.conicet.gov.ar:11336/117684
network_acronym_str AR
network_name_str Argentina
repository_id_str
dc.title.none.fl_str_mv Increased cholesterol efflux capacity in metabolic syndrome: Relation with qualitative alterations in HDL and LCAT
title Increased cholesterol efflux capacity in metabolic syndrome: Relation with qualitative alterations in HDL and LCAT
spellingShingle Increased cholesterol efflux capacity in metabolic syndrome: Relation with qualitative alterations in HDL and LCAT
Lucero, Diego Martín
CHOLESTEROL EFFLUX CAPACITY
HDL
LCAT
METABOLIC SYNDROME
PRE-Β1-HDL
https://purl.org/becyt/ford/3.3
https://purl.org/becyt/ford/3
title_short Increased cholesterol efflux capacity in metabolic syndrome: Relation with qualitative alterations in HDL and LCAT
title_full Increased cholesterol efflux capacity in metabolic syndrome: Relation with qualitative alterations in HDL and LCAT
title_fullStr Increased cholesterol efflux capacity in metabolic syndrome: Relation with qualitative alterations in HDL and LCAT
title_full_unstemmed Increased cholesterol efflux capacity in metabolic syndrome: Relation with qualitative alterations in HDL and LCAT
title_sort Increased cholesterol efflux capacity in metabolic syndrome: Relation with qualitative alterations in HDL and LCAT
dc.creator.none.fl_str_mv Lucero, Diego Martín
Svidirov, Denis
Freeman, Lita
López, Graciela I.
Fassio, Eduardo
Remaley, Alan T.
Schreier, Laura Ester
author Lucero, Diego Martín
author_facet Lucero, Diego Martín
Svidirov, Denis
Freeman, Lita
López, Graciela I.
Fassio, Eduardo
Remaley, Alan T.
Schreier, Laura Ester
author_role author
author2 Svidirov, Denis
Freeman, Lita
López, Graciela I.
Fassio, Eduardo
Remaley, Alan T.
Schreier, Laura Ester
author2_role author
author
author
author
author
author
dc.subject.none.fl_str_mv CHOLESTEROL EFFLUX CAPACITY
HDL
LCAT
METABOLIC SYNDROME
PRE-Β1-HDL
https://purl.org/becyt/ford/3.3
https://purl.org/becyt/ford/3
topic CHOLESTEROL EFFLUX CAPACITY
HDL
LCAT
METABOLIC SYNDROME
PRE-Β1-HDL
https://purl.org/becyt/ford/3.3
https://purl.org/becyt/ford/3
description Background: Metabolic syndrome (MetS) is associated with changes in HDL levels, composition and sub-fraction profile. Whether these alterations affect HDL anti-atherogenic function, specifically measured as its capacity to perform cholesterol efflux, is not yet clearly known. Objective: To evaluate the relation between serum cholesterol efflux capacity and the changes in HDL composition and sub-fraction profile in MetS. Methods: In 35 non-treated MetS patients and 15 healthy controls, HDL mediated cholesterol efflux was measured as the ability of apoB-depleted serum to accept cholesterol from cholesterol-loaded BHK cells expressing either ABCA1 or ABCG1. Additionally we determined: lipid profile, HDL sub-fractions (NMR) and LCAT mass (ELISA). Isolated HDL (δ:1.063-1.210 g/mL) was chemically characterized. Pre-β1-HDL was determined by 2D-electrophoresis in a sub-group of MetS and controls (n = 6 each). Results: Surprisingly, MetS patients presented higher ABCA1 mediated cholesterol efflux (10.4 ± 1.8 vs. 8.7 ± 0.3%; p = 0.0001), without differences in ABCG1 efflux. In MetS, HDL showed reduction in particle size and number (p < 0.02) and lower large/small HDL ratio (p = 0.05), as well as triglyceride enrichment (p = 0.0001). Pre-β1-HDL was increased in MetS (p = 0.048) and correlated with ABCA1-cholesterol efflux (r = 0.64; p = 0.042). LCAT mass showed a tendency to reduction in MetS (p = 0.08), and inversely correlated with ABCA1-cholesterol efflux (r = -0.51; p = 0.001), independently of obesity and insulin-resistance (β = -0.40, p = 0.034). Conclusion: This is the first description of ABCA1 mediated cholesterol efflux in MetS. Regardless the reduced HDL-cholesterol, in vitro cholesterol efflux capacity by ABCA1 was enhanced, linked to increased pre-β1-HDL and slightly reduced in LCAT mass that would probably reflect a delay in reverse cholesterol transport occurring in MetS.
publishDate 2015
dc.date.none.fl_str_mv 2015-09
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/117684
Lucero, Diego Martín; Svidirov, Denis; Freeman, Lita; López, Graciela I.; Fassio, Eduardo; et al.; Increased cholesterol efflux capacity in metabolic syndrome: Relation with qualitative alterations in HDL and LCAT; Elsevier Ireland; Atherosclerosis; 242; 1; 9-2015; 236-242
0021-9150
CONICET Digital
CONICET
url http://hdl.handle.net/11336/117684
identifier_str_mv Lucero, Diego Martín; Svidirov, Denis; Freeman, Lita; López, Graciela I.; Fassio, Eduardo; et al.; Increased cholesterol efflux capacity in metabolic syndrome: Relation with qualitative alterations in HDL and LCAT; Elsevier Ireland; Atherosclerosis; 242; 1; 9-2015; 236-242
0021-9150
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/url/https://www.sciencedirect.com/science/article/abs/pii/S0021915015300393
info:eu-repo/semantics/altIdentifier/doi/10.1016/j.atherosclerosis.2015.07.019
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
application/pdf
application/pdf
dc.publisher.none.fl_str_mv Elsevier Ireland
publisher.none.fl_str_mv Elsevier Ireland
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
_version_ 1799194924902514688
spelling Increased cholesterol efflux capacity in metabolic syndrome: Relation with qualitative alterations in HDL and LCATLucero, Diego MartínSvidirov, DenisFreeman, LitaLópez, Graciela I.Fassio, EduardoRemaley, Alan T.Schreier, Laura EsterCHOLESTEROL EFFLUX CAPACITYHDLLCATMETABOLIC SYNDROMEPRE-Β1-HDLhttps://purl.org/becyt/ford/3.3https://purl.org/becyt/ford/3Background: Metabolic syndrome (MetS) is associated with changes in HDL levels, composition and sub-fraction profile. Whether these alterations affect HDL anti-atherogenic function, specifically measured as its capacity to perform cholesterol efflux, is not yet clearly known. Objective: To evaluate the relation between serum cholesterol efflux capacity and the changes in HDL composition and sub-fraction profile in MetS. Methods: In 35 non-treated MetS patients and 15 healthy controls, HDL mediated cholesterol efflux was measured as the ability of apoB-depleted serum to accept cholesterol from cholesterol-loaded BHK cells expressing either ABCA1 or ABCG1. Additionally we determined: lipid profile, HDL sub-fractions (NMR) and LCAT mass (ELISA). Isolated HDL (δ:1.063-1.210 g/mL) was chemically characterized. Pre-β1-HDL was determined by 2D-electrophoresis in a sub-group of MetS and controls (n = 6 each). Results: Surprisingly, MetS patients presented higher ABCA1 mediated cholesterol efflux (10.4 ± 1.8 vs. 8.7 ± 0.3%; p = 0.0001), without differences in ABCG1 efflux. In MetS, HDL showed reduction in particle size and number (p < 0.02) and lower large/small HDL ratio (p = 0.05), as well as triglyceride enrichment (p = 0.0001). Pre-β1-HDL was increased in MetS (p = 0.048) and correlated with ABCA1-cholesterol efflux (r = 0.64; p = 0.042). LCAT mass showed a tendency to reduction in MetS (p = 0.08), and inversely correlated with ABCA1-cholesterol efflux (r = -0.51; p = 0.001), independently of obesity and insulin-resistance (β = -0.40, p = 0.034). Conclusion: This is the first description of ABCA1 mediated cholesterol efflux in MetS. Regardless the reduced HDL-cholesterol, in vitro cholesterol efflux capacity by ABCA1 was enhanced, linked to increased pre-β1-HDL and slightly reduced in LCAT mass that would probably reflect a delay in reverse cholesterol transport occurring in MetS.Fil: Lucero, Diego Martín. Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica. Instituto de Fisiopatología y Bioquímica Clínica; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay; ArgentinaFil: Svidirov, Denis. National Institutes of Health; Estados UnidosFil: Freeman, Lita. National Institutes of Health; Estados UnidosFil: López, Graciela I.. Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica. Instituto de Fisiopatología y Bioquímica Clínica; ArgentinaFil: Fassio, Eduardo. Hospital Nacional Profesor Alejandro Posadas; ArgentinaFil: Remaley, Alan T.. National Institutes of Health; Estados UnidosFil: Schreier, Laura Ester. Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica. Instituto de Fisiopatología y Bioquímica Clínica; ArgentinaElsevier Ireland2015-09info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/117684Lucero, Diego Martín; Svidirov, Denis; Freeman, Lita; López, Graciela I.; Fassio, Eduardo; et al.; Increased cholesterol efflux capacity in metabolic syndrome: Relation with qualitative alterations in HDL and LCAT; Elsevier Ireland; Atherosclerosis; 242; 1; 9-2015; 236-2420021-9150CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/url/https://www.sciencedirect.com/science/article/abs/pii/S0021915015300393info:eu-repo/semantics/altIdentifier/doi/10.1016/j.atherosclerosis.2015.07.019info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2024-05-08T13:39:15Zoai:ri.conicet.gov.ar:11336/117684instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982024-05-08 13:39:15.596CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
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