The pluripotency transcription factor Nanog represses glutathione reductase gene expression in mouse embryonic stem cells

Objective: Redox homeostasis maintenance is essential to bring about cellular functions. Particularly, embryonic stem cells (ESCs) have high fidelity mechanisms for DNA repair, high activity of different antioxidant enzymes and low levels of oxidative stress. Although the expression and activity of...

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Autores: Solari, Claudia María, Petrone Parcero, María Victoria, Toro, Ayelen Rayen, Vazquez Echegaray, Camila, Cosentino, María Soledad, Waisman, Ariel, Francia, Marcos Gabriel, Barañao, Jose Lino Salvador, Miriuka, Santiago Gabriel, Guberman, Alejandra Sonia
Tipo de recurso: artículo
Estado:Versión publicada
Fecha de publicación:2019
País:Argentina
Institución:Consejo Nacional de Investigaciones Científicas y Técnicas
Repositorio:CONICET Digital (CONICET)
Idioma:inglés
OAI Identifier:oai:ri.conicet.gov.ar:11336/119834
Acceso en línea:http://hdl.handle.net/11336/119834
Access Level:acceso abierto
Palabra clave:DIFFERENTIATION
EMBRYONIC STEM CELLS
GENE EXPRESSION
GLUTATHIONE REDUCTASE
NANOG
REDOX HOMEOSTASIS
TRANSCRIPTIONAL REGULATION
https://purl.org/becyt/ford/1.6
https://purl.org/becyt/ford/1
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dc.title.none.fl_str_mv The pluripotency transcription factor Nanog represses glutathione reductase gene expression in mouse embryonic stem cells
title The pluripotency transcription factor Nanog represses glutathione reductase gene expression in mouse embryonic stem cells
spellingShingle The pluripotency transcription factor Nanog represses glutathione reductase gene expression in mouse embryonic stem cells
Solari, Claudia María
DIFFERENTIATION
EMBRYONIC STEM CELLS
GENE EXPRESSION
GLUTATHIONE REDUCTASE
NANOG
REDOX HOMEOSTASIS
TRANSCRIPTIONAL REGULATION
https://purl.org/becyt/ford/1.6
https://purl.org/becyt/ford/1
title_short The pluripotency transcription factor Nanog represses glutathione reductase gene expression in mouse embryonic stem cells
title_full The pluripotency transcription factor Nanog represses glutathione reductase gene expression in mouse embryonic stem cells
title_fullStr The pluripotency transcription factor Nanog represses glutathione reductase gene expression in mouse embryonic stem cells
title_full_unstemmed The pluripotency transcription factor Nanog represses glutathione reductase gene expression in mouse embryonic stem cells
title_sort The pluripotency transcription factor Nanog represses glutathione reductase gene expression in mouse embryonic stem cells
dc.creator.none.fl_str_mv Solari, Claudia María
Petrone Parcero, María Victoria
Toro, Ayelen Rayen
Vazquez Echegaray, Camila
Cosentino, María Soledad
Waisman, Ariel
Francia, Marcos Gabriel
Barañao, Jose Lino Salvador
Miriuka, Santiago Gabriel
Guberman, Alejandra Sonia
author Solari, Claudia María
author_facet Solari, Claudia María
Petrone Parcero, María Victoria
Toro, Ayelen Rayen
Vazquez Echegaray, Camila
Cosentino, María Soledad
Waisman, Ariel
Francia, Marcos Gabriel
Barañao, Jose Lino Salvador
Miriuka, Santiago Gabriel
Guberman, Alejandra Sonia
author_role author
author2 Petrone Parcero, María Victoria
Toro, Ayelen Rayen
Vazquez Echegaray, Camila
Cosentino, María Soledad
Waisman, Ariel
Francia, Marcos Gabriel
Barañao, Jose Lino Salvador
Miriuka, Santiago Gabriel
Guberman, Alejandra Sonia
author2_role author
author
author
author
author
author
author
author
author
dc.subject.none.fl_str_mv DIFFERENTIATION
EMBRYONIC STEM CELLS
GENE EXPRESSION
GLUTATHIONE REDUCTASE
NANOG
REDOX HOMEOSTASIS
TRANSCRIPTIONAL REGULATION
https://purl.org/becyt/ford/1.6
https://purl.org/becyt/ford/1
topic DIFFERENTIATION
EMBRYONIC STEM CELLS
GENE EXPRESSION
GLUTATHIONE REDUCTASE
NANOG
REDOX HOMEOSTASIS
TRANSCRIPTIONAL REGULATION
https://purl.org/becyt/ford/1.6
https://purl.org/becyt/ford/1
description Objective: Redox homeostasis maintenance is essential to bring about cellular functions. Particularly, embryonic stem cells (ESCs) have high fidelity mechanisms for DNA repair, high activity of different antioxidant enzymes and low levels of oxidative stress. Although the expression and activity of antioxidant enzymes are reduced throughout the differentiation, the knowledge about the transcriptional regulation of genes involved in defense against oxidative stress is yet restricted. Since glutathione is a central component of a complex system involved in preserving cellular redox status, we aimed to study whether the expression of the glutathione reductase (Gsr) gene, which encodes an essential enzyme for cellular redox homeostasis, is modulated by the transcription factors critical for self-renewal and pluripotency of ESCs. Results: We found that Gsr gene is expressed in ESCs during the pluripotent state and it was upregulated when these cells were induced to differentiate, concomitantly with Nanog decreased expression. Moreover, we found an increase in Gsr mRNA levels when Nanog was downregulated by a specific shRNA targeting this transcription factor in ESCs. Our results suggest that Nanog represses Gsr gene expression in ESCs, evidencing a role of this crucial pluripotency transcription factor in preservation of redox homeostasis in stem cells.
publishDate 2019
dc.date.none.fl_str_mv 2019-07
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/119834
Solari, Claudia María; Petrone Parcero, María Victoria; Toro, Ayelen Rayen; Vazquez Echegaray, Camila; Cosentino, María Soledad; et al.; The pluripotency transcription factor Nanog represses glutathione reductase gene expression in mouse embryonic stem cells; BioMed Central; BMC Research Notes; 12; 1; 7-2019; 1-7
1756-0500
CONICET Digital
CONICET
url http://hdl.handle.net/11336/119834
identifier_str_mv Solari, Claudia María; Petrone Parcero, María Victoria; Toro, Ayelen Rayen; Vazquez Echegaray, Camila; Cosentino, María Soledad; et al.; The pluripotency transcription factor Nanog represses glutathione reductase gene expression in mouse embryonic stem cells; BioMed Central; BMC Research Notes; 12; 1; 7-2019; 1-7
1756-0500
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/url/https://bmcresnotes.biomedcentral.com/articles/10.1186/s13104-019-4411-0
info:eu-repo/semantics/altIdentifier/doi/10.1186/s13104-019-4411-0
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
application/pdf
application/pdf
dc.publisher.none.fl_str_mv BioMed Central
publisher.none.fl_str_mv BioMed Central
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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spelling The pluripotency transcription factor Nanog represses glutathione reductase gene expression in mouse embryonic stem cellsSolari, Claudia MaríaPetrone Parcero, María VictoriaToro, Ayelen RayenVazquez Echegaray, CamilaCosentino, María SoledadWaisman, ArielFrancia, Marcos GabrielBarañao, Jose Lino SalvadorMiriuka, Santiago GabrielGuberman, Alejandra SoniaDIFFERENTIATIONEMBRYONIC STEM CELLSGENE EXPRESSIONGLUTATHIONE REDUCTASENANOGREDOX HOMEOSTASISTRANSCRIPTIONAL REGULATIONhttps://purl.org/becyt/ford/1.6https://purl.org/becyt/ford/1Objective: Redox homeostasis maintenance is essential to bring about cellular functions. Particularly, embryonic stem cells (ESCs) have high fidelity mechanisms for DNA repair, high activity of different antioxidant enzymes and low levels of oxidative stress. Although the expression and activity of antioxidant enzymes are reduced throughout the differentiation, the knowledge about the transcriptional regulation of genes involved in defense against oxidative stress is yet restricted. Since glutathione is a central component of a complex system involved in preserving cellular redox status, we aimed to study whether the expression of the glutathione reductase (Gsr) gene, which encodes an essential enzyme for cellular redox homeostasis, is modulated by the transcription factors critical for self-renewal and pluripotency of ESCs. Results: We found that Gsr gene is expressed in ESCs during the pluripotent state and it was upregulated when these cells were induced to differentiate, concomitantly with Nanog decreased expression. Moreover, we found an increase in Gsr mRNA levels when Nanog was downregulated by a specific shRNA targeting this transcription factor in ESCs. Our results suggest that Nanog represses Gsr gene expression in ESCs, evidencing a role of this crucial pluripotency transcription factor in preservation of redox homeostasis in stem cells.Fil: Solari, Claudia María. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Ciudad Universitaria. Instituto de Química Biológica de la Facultad de Ciencias Exactas y Naturales. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Instituto de Química Biológica de la Facultad de Ciencias Exactas y Naturales; ArgentinaFil: Petrone Parcero, María Victoria. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Ciudad Universitaria. Instituto de Química Biológica de la Facultad de Ciencias Exactas y Naturales. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Instituto de Química Biológica de la Facultad de Ciencias Exactas y Naturales; ArgentinaFil: Toro, Ayelen Rayen. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Ciudad Universitaria. Instituto de Química Biológica de la Facultad de Ciencias Exactas y Naturales. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Instituto de Química Biológica de la Facultad de Ciencias Exactas y Naturales; ArgentinaFil: Vazquez Echegaray, Camila. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Ciudad Universitaria. Instituto de Química Biológica de la Facultad de Ciencias Exactas y Naturales. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Instituto de Química Biológica de la Facultad de Ciencias Exactas y Naturales; ArgentinaFil: Cosentino, María Soledad. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Ciudad Universitaria. Instituto de Química Biológica de la Facultad de Ciencias Exactas y Naturales. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Instituto de Química Biológica de la Facultad de Ciencias Exactas y Naturales; ArgentinaFil: Waisman, Ariel. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Ciudad Universitaria. Instituto de Química Biológica de la Facultad de Ciencias Exactas y Naturales. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Instituto de Química Biológica de la Facultad de Ciencias Exactas y Naturales; ArgentinaFil: Francia, Marcos Gabriel. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Ciudad Universitaria. Instituto de Química Biológica de la Facultad de Ciencias Exactas y Naturales. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Instituto de Química Biológica de la Facultad de Ciencias Exactas y Naturales; ArgentinaFil: Barañao, Jose Lino Salvador. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Departamento de Química Biológica; ArgentinaFil: Miriuka, Santiago Gabriel. Fundación para la Lucha contra las Enfermedades Neurológicas de la Infancia; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaFil: Guberman, Alejandra Sonia. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Ciudad Universitaria. Instituto de Química Biológica de la Facultad de Ciencias Exactas y Naturales. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Instituto de Química Biológica de la Facultad de Ciencias Exactas y Naturales; ArgentinaBioMed Central2019-07info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/119834Solari, Claudia María; Petrone Parcero, María Victoria; Toro, Ayelen Rayen; Vazquez Echegaray, Camila; Cosentino, María Soledad; et al.; The pluripotency transcription factor Nanog represses glutathione reductase gene expression in mouse embryonic stem cells; BioMed Central; BMC Research Notes; 12; 1; 7-2019; 1-71756-0500CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/url/https://bmcresnotes.biomedcentral.com/articles/10.1186/s13104-019-4411-0info:eu-repo/semantics/altIdentifier/doi/10.1186/s13104-019-4411-0info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2024-05-08T13:52:12Zoai:ri.conicet.gov.ar:11336/119834instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982024-05-08 13:52:13.278CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
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