Cloning and functional characterization of two key enzymes of glycosphingolipid biosynthesis in the amphibian Xenopus laevis

Gangliosides are a subfamily of complex glycosphingolipids (GSLs) with important roles in many biological processes. In this study, we report the cDNA cloning, functional characterization, and the spatial and temporal expression of Xlcgt and Xlgd3 synthase during Xenopus laevis development. Xlcgt wa...

Descripción completa

Detalles Bibliográficos
Autores: Luque, Melchor Emilio, Crespo, Pilar María, Mónaco, María Elvira, Aybar, Manuel Javier, Daniotti, Jose Luis, Sanchez, Sara Serafina del V.
Tipo de recurso: artículo
Estado:Versión publicada
Fecha de publicación:2008
País:Argentina
Institución:Consejo Nacional de Investigaciones Científicas y Técnicas
Repositorio:CONICET Digital (CONICET)
Idioma:inglés
OAI Identifier:oai:ri.conicet.gov.ar:11336/65686
Acceso en línea:http://hdl.handle.net/11336/65686
Access Level:acceso abierto
Palabra clave:AMPHIBIAN
GLYCOSPHINGOLIPIDS
MESODERMAL LAYER
https://purl.org/becyt/ford/1.6
https://purl.org/becyt/ford/1
id AR_7c3a2e6fbd86fbd1051533fdb1aef898
oai_identifier_str oai:ri.conicet.gov.ar:11336/65686
network_acronym_str AR
network_name_str Argentina
repository_id_str
dc.title.none.fl_str_mv Cloning and functional characterization of two key enzymes of glycosphingolipid biosynthesis in the amphibian Xenopus laevis
title Cloning and functional characterization of two key enzymes of glycosphingolipid biosynthesis in the amphibian Xenopus laevis
spellingShingle Cloning and functional characterization of two key enzymes of glycosphingolipid biosynthesis in the amphibian Xenopus laevis
Luque, Melchor Emilio
AMPHIBIAN
GLYCOSPHINGOLIPIDS
MESODERMAL LAYER
https://purl.org/becyt/ford/1.6
https://purl.org/becyt/ford/1
title_short Cloning and functional characterization of two key enzymes of glycosphingolipid biosynthesis in the amphibian Xenopus laevis
title_full Cloning and functional characterization of two key enzymes of glycosphingolipid biosynthesis in the amphibian Xenopus laevis
title_fullStr Cloning and functional characterization of two key enzymes of glycosphingolipid biosynthesis in the amphibian Xenopus laevis
title_full_unstemmed Cloning and functional characterization of two key enzymes of glycosphingolipid biosynthesis in the amphibian Xenopus laevis
title_sort Cloning and functional characterization of two key enzymes of glycosphingolipid biosynthesis in the amphibian Xenopus laevis
dc.creator.none.fl_str_mv Luque, Melchor Emilio
Crespo, Pilar María
Mónaco, María Elvira
Aybar, Manuel Javier
Daniotti, Jose Luis
Sanchez, Sara Serafina del V.
author Luque, Melchor Emilio
author_facet Luque, Melchor Emilio
Crespo, Pilar María
Mónaco, María Elvira
Aybar, Manuel Javier
Daniotti, Jose Luis
Sanchez, Sara Serafina del V.
author_role author
author2 Crespo, Pilar María
Mónaco, María Elvira
Aybar, Manuel Javier
Daniotti, Jose Luis
Sanchez, Sara Serafina del V.
author2_role author
author
author
author
author
dc.subject.none.fl_str_mv AMPHIBIAN
GLYCOSPHINGOLIPIDS
MESODERMAL LAYER
https://purl.org/becyt/ford/1.6
https://purl.org/becyt/ford/1
topic AMPHIBIAN
GLYCOSPHINGOLIPIDS
MESODERMAL LAYER
https://purl.org/becyt/ford/1.6
https://purl.org/becyt/ford/1
description Gangliosides are a subfamily of complex glycosphingolipids (GSLs) with important roles in many biological processes. In this study, we report the cDNA cloning, functional characterization, and the spatial and temporal expression of Xlcgt and Xlgd3 synthase during Xenopus laevis development. Xlcgt was expressed both maternally and zigotically persisting at least until stage 35. Maternal Xlgd3 synthase mRNA could not be detected and showed a steady-state expression from gastrula to late tailbud stage. Xlcgt is mainly present in involuted paraxial mesoderm, neural folds, and their derivatives. Xlgd3 synthase transcripts were detected in the dorsal blastoporal lip, in the presumptive neuroectoderm, and later in the head region, branchial arches, otic and optic primordia. We determined the effect of glycosphingolipid depletion with 1-phenyl-2-palmitoyl-3-morpholino-1-propanol (PPMP) in mesodermal layer. PPMP-injected embryos showed altered expression domains in the mesodermal markers. Our results suggest that GSL are involved in convergent-extension movements during early development in Xenopus. © 2007 Wiley-Liss, Inc.
publishDate 2008
dc.date.none.fl_str_mv 2008-01
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/65686
Luque, Melchor Emilio; Crespo, Pilar María; Mónaco, María Elvira; Aybar, Manuel Javier; Daniotti, Jose Luis; et al.; Cloning and functional characterization of two key enzymes of glycosphingolipid biosynthesis in the amphibian Xenopus laevis; Wiley-liss, Div John Wiley & Sons Inc; Developmental Dynamics; 237; 1; 1-2008; 112-123
1058-8388
CONICET Digital
CONICET
url http://hdl.handle.net/11336/65686
identifier_str_mv Luque, Melchor Emilio; Crespo, Pilar María; Mónaco, María Elvira; Aybar, Manuel Javier; Daniotti, Jose Luis; et al.; Cloning and functional characterization of two key enzymes of glycosphingolipid biosynthesis in the amphibian Xenopus laevis; Wiley-liss, Div John Wiley & Sons Inc; Developmental Dynamics; 237; 1; 1-2008; 112-123
1058-8388
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/doi/10.1002/dvdy.21406
info:eu-repo/semantics/altIdentifier/url/https://onlinelibrary.wiley.com/doi/full/10.1002/dvdy.21406
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
application/pdf
application/pdf
application/pdf
application/pdf
application/pdf
dc.publisher.none.fl_str_mv Wiley-liss, Div John Wiley & Sons Inc
publisher.none.fl_str_mv Wiley-liss, Div John Wiley & Sons Inc
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
_version_ 1799196246286532608
spelling Cloning and functional characterization of two key enzymes of glycosphingolipid biosynthesis in the amphibian Xenopus laevisLuque, Melchor EmilioCrespo, Pilar MaríaMónaco, María ElviraAybar, Manuel JavierDaniotti, Jose LuisSanchez, Sara Serafina del V.AMPHIBIANGLYCOSPHINGOLIPIDSMESODERMAL LAYERhttps://purl.org/becyt/ford/1.6https://purl.org/becyt/ford/1Gangliosides are a subfamily of complex glycosphingolipids (GSLs) with important roles in many biological processes. In this study, we report the cDNA cloning, functional characterization, and the spatial and temporal expression of Xlcgt and Xlgd3 synthase during Xenopus laevis development. Xlcgt was expressed both maternally and zigotically persisting at least until stage 35. Maternal Xlgd3 synthase mRNA could not be detected and showed a steady-state expression from gastrula to late tailbud stage. Xlcgt is mainly present in involuted paraxial mesoderm, neural folds, and their derivatives. Xlgd3 synthase transcripts were detected in the dorsal blastoporal lip, in the presumptive neuroectoderm, and later in the head region, branchial arches, otic and optic primordia. We determined the effect of glycosphingolipid depletion with 1-phenyl-2-palmitoyl-3-morpholino-1-propanol (PPMP) in mesodermal layer. PPMP-injected embryos showed altered expression domains in the mesodermal markers. Our results suggest that GSL are involved in convergent-extension movements during early development in Xenopus. © 2007 Wiley-Liss, Inc.Fil: Luque, Melchor Emilio. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Instituto Superior de Investigaciones Biológicas. Universidad Nacional de Tucumán. Instituto Superior de Investigaciones Biológicas; ArgentinaFil: Crespo, Pilar María. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba. Centro de Investigaciones en Química Biológica de Córdoba. Universidad Nacional de Córdoba. Facultad de Ciencias Químicas. Centro de Investigaciones en Química Biológica de Córdoba; ArgentinaFil: Mónaco, María Elvira. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Instituto Superior de Investigaciones Biológicas. Universidad Nacional de Tucumán. Instituto Superior de Investigaciones Biológicas; ArgentinaFil: Aybar, Manuel Javier. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Instituto Superior de Investigaciones Biológicas. Universidad Nacional de Tucumán. Instituto Superior de Investigaciones Biológicas; ArgentinaFil: Daniotti, Jose Luis. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba. Centro de Investigaciones en Química Biológica de Córdoba. Universidad Nacional de Córdoba. Facultad de Ciencias Químicas. Centro de Investigaciones en Química Biológica de Córdoba; ArgentinaFil: Sanchez, Sara Serafina del V.. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Instituto Superior de Investigaciones Biológicas. Universidad Nacional de Tucumán. Instituto Superior de Investigaciones Biológicas; ArgentinaWiley-liss, Div John Wiley & Sons Inc2008-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfapplication/pdfapplication/pdfapplication/pdfapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/65686Luque, Melchor Emilio; Crespo, Pilar María; Mónaco, María Elvira; Aybar, Manuel Javier; Daniotti, Jose Luis; et al.; Cloning and functional characterization of two key enzymes of glycosphingolipid biosynthesis in the amphibian Xenopus laevis; Wiley-liss, Div John Wiley & Sons Inc; Developmental Dynamics; 237; 1; 1-2008; 112-1231058-8388CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/doi/10.1002/dvdy.21406info:eu-repo/semantics/altIdentifier/url/https://onlinelibrary.wiley.com/doi/full/10.1002/dvdy.21406info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2024-05-08T14:20:15Zoai:ri.conicet.gov.ar:11336/65686instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982024-05-08 14:20:16.083CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
score 15,812429

Similares en LA Referencia