Transcriptional profiling of human macrophages during infection with <i>Bordetella pertussis</i>

<i>Bordetella pertussis</i>, a strictly human re-emerging pathogen and the causative agent of whooping cough, exploits a broad variety of virulence factors to establish efficient infection. Here, we used RNA sequencing to analyse the changes in gene expression profiles of human THP-1 mac...

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Autores: Petráčková, Denisa, Farman, Mariam R., Amman, Fabian, Linhartová, Irena, Dienstbier, Ana, Kumar, Dilip, Držmíšek, Jakub, Hofacker, Ivo, Rodríguez, María Eugenia, Večerek, Branislav
Tipo de recurso: artículo
Estado:Versión publicada
Fecha de publicación:2020
País:Argentina
Institución:Universidad Nacional de La Plata
Repositorio:SEDICI (UNLP)
Idioma:inglés
OAI Identifier:oai:sedici.unlp.edu.ar:10915/107929
Acceso en línea:http://sedici.unlp.edu.ar/handle/10915/107929
Access Level:acceso abierto
Palabra clave:Ciencias Exactas
Biología
Bordetella pertussis
infection
macrophage
intracellular survival
host-pathogen interaction
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spelling Transcriptional profiling of human macrophages during infection with <i>Bordetella pertussis</i>Petráčková, DenisaFarman, Mariam R.Amman, FabianLinhartová, IrenaDienstbier, AnaKumar, DilipDržmíšek, JakubHofacker, IvoRodríguez, María EugeniaVečerek, BranislavCiencias ExactasBiologíaBordetella pertussisinfectionmacrophageintracellular survivalhost-pathogen interaction<i>Bordetella pertussis</i>, a strictly human re-emerging pathogen and the causative agent of whooping cough, exploits a broad variety of virulence factors to establish efficient infection. Here, we used RNA sequencing to analyse the changes in gene expression profiles of human THP-1 macrophages resulting from <i>B. pertussis</i> infection. In parallel, we attempted to determine the changes in intracellular <i>B. pertussis</i>-specific transcriptomic profiles resulting from interaction with macrophages. Our analysis revealed that global gene expression profiles in THP-1 macrophages are extensively rewired 6 h post-infection. Among the highly expressed genes, we identified those encoding cytokines, chemokines, and transcription regulators involved in the induction of the M1 and M2 macrophage polarization programmes. Notably, several host genes involved in the control of apoptosis and inflammation which are known to be hijacked by intracellular bacterial pathogens were overexpressed upon infection. Furthermore, <i>in silico</i> analyses identified large temporal changes in expression of specific gene subsets involved in signalling and metabolic pathways. Despite limited numbers of the bacterial reads, we observed reduced expression of majority of virulence factors and upregulation of several transcriptional regulators during infection suggesting that intracellular <i>B. pertussis</i> cells switch from virulent to avirulent phase and actively adapt to intracellular environment, respectively.Facultad de Ciencias ExactasCentro de Investigación y Desarrollo en Fermentaciones Industriales2020info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionArticulohttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdf731-742http://sedici.unlp.edu.ar/handle/10915/107929enginfo:eu-repo/semantics/altIdentifier/url/http://europepmc.org/backend/ptpmcrender.fcgi?accid=PMC7237194&blobtype=pdfinfo:eu-repo/semantics/altIdentifier/url/https://www.tandfonline.com/doi/full/10.1080/15476286.2020.1727694info:eu-repo/semantics/altIdentifier/issn/1555-8584info:eu-repo/semantics/altIdentifier/pmid/32070192info:eu-repo/semantics/altIdentifier/doi/10.1080/15476286.2020.1727694info:eu-repo/semantics/openAccesshttp://creativecommons.org/licenses/by/4.0/Creative Commons Attribution 4.0 International (CC BY 4.0)reponame:SEDICI (UNLP)instname:Universidad Nacional de La Platainstacron:UNLP2024-05-08T13:02:02Zoai:sedici.unlp.edu.ar:10915/107929Institucionalhttp://sedici.unlp.edu.ar/Universidad públicaNo correspondehttp://sedici.unlp.edu.ar/oai/snrdalira@sedici.unlp.edu.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:13292024-05-08 13:02:02.733SEDICI (UNLP) - Universidad Nacional de La Platafalse
dc.title.none.fl_str_mv Transcriptional profiling of human macrophages during infection with <i>Bordetella pertussis</i>
title Transcriptional profiling of human macrophages during infection with <i>Bordetella pertussis</i>
spellingShingle Transcriptional profiling of human macrophages during infection with <i>Bordetella pertussis</i>
Petráčková, Denisa
Ciencias Exactas
Biología
Bordetella pertussis
infection
macrophage
intracellular survival
host-pathogen interaction
title_short Transcriptional profiling of human macrophages during infection with <i>Bordetella pertussis</i>
title_full Transcriptional profiling of human macrophages during infection with <i>Bordetella pertussis</i>
title_fullStr Transcriptional profiling of human macrophages during infection with <i>Bordetella pertussis</i>
title_full_unstemmed Transcriptional profiling of human macrophages during infection with <i>Bordetella pertussis</i>
title_sort Transcriptional profiling of human macrophages during infection with <i>Bordetella pertussis</i>
dc.creator.none.fl_str_mv Petráčková, Denisa
Farman, Mariam R.
Amman, Fabian
Linhartová, Irena
Dienstbier, Ana
Kumar, Dilip
Držmíšek, Jakub
Hofacker, Ivo
Rodríguez, María Eugenia
Večerek, Branislav
author Petráčková, Denisa
author_facet Petráčková, Denisa
Farman, Mariam R.
Amman, Fabian
Linhartová, Irena
Dienstbier, Ana
Kumar, Dilip
Držmíšek, Jakub
Hofacker, Ivo
Rodríguez, María Eugenia
Večerek, Branislav
author_role author
author2 Farman, Mariam R.
Amman, Fabian
Linhartová, Irena
Dienstbier, Ana
Kumar, Dilip
Držmíšek, Jakub
Hofacker, Ivo
Rodríguez, María Eugenia
Večerek, Branislav
author2_role author
author
author
author
author
author
author
author
author
dc.subject.none.fl_str_mv Ciencias Exactas
Biología
Bordetella pertussis
infection
macrophage
intracellular survival
host-pathogen interaction
topic Ciencias Exactas
Biología
Bordetella pertussis
infection
macrophage
intracellular survival
host-pathogen interaction
description <i>Bordetella pertussis</i>, a strictly human re-emerging pathogen and the causative agent of whooping cough, exploits a broad variety of virulence factors to establish efficient infection. Here, we used RNA sequencing to analyse the changes in gene expression profiles of human THP-1 macrophages resulting from <i>B. pertussis</i> infection. In parallel, we attempted to determine the changes in intracellular <i>B. pertussis</i>-specific transcriptomic profiles resulting from interaction with macrophages. Our analysis revealed that global gene expression profiles in THP-1 macrophages are extensively rewired 6 h post-infection. Among the highly expressed genes, we identified those encoding cytokines, chemokines, and transcription regulators involved in the induction of the M1 and M2 macrophage polarization programmes. Notably, several host genes involved in the control of apoptosis and inflammation which are known to be hijacked by intracellular bacterial pathogens were overexpressed upon infection. Furthermore, <i>in silico</i> analyses identified large temporal changes in expression of specific gene subsets involved in signalling and metabolic pathways. Despite limited numbers of the bacterial reads, we observed reduced expression of majority of virulence factors and upregulation of several transcriptional regulators during infection suggesting that intracellular <i>B. pertussis</i> cells switch from virulent to avirulent phase and actively adapt to intracellular environment, respectively.
publishDate 2020
dc.date.none.fl_str_mv 2020
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
Articulo
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://sedici.unlp.edu.ar/handle/10915/107929
url http://sedici.unlp.edu.ar/handle/10915/107929
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/url/http://europepmc.org/backend/ptpmcrender.fcgi?accid=PMC7237194&blobtype=pdf
info:eu-repo/semantics/altIdentifier/url/https://www.tandfonline.com/doi/full/10.1080/15476286.2020.1727694
info:eu-repo/semantics/altIdentifier/issn/1555-8584
info:eu-repo/semantics/altIdentifier/pmid/32070192
info:eu-repo/semantics/altIdentifier/doi/10.1080/15476286.2020.1727694
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
http://creativecommons.org/licenses/by/4.0/
Creative Commons Attribution 4.0 International (CC BY 4.0)
eu_rights_str_mv openAccess
rights_invalid_str_mv http://creativecommons.org/licenses/by/4.0/
Creative Commons Attribution 4.0 International (CC BY 4.0)
dc.format.none.fl_str_mv application/pdf
731-742
dc.source.none.fl_str_mv reponame:SEDICI (UNLP)
instname:Universidad Nacional de La Plata
instacron:UNLP
instname_str Universidad Nacional de La Plata
instacron_str UNLP
institution UNLP
reponame_str SEDICI (UNLP)
collection SEDICI (UNLP)
repository.name.fl_str_mv SEDICI (UNLP) - Universidad Nacional de La Plata
repository.mail.fl_str_mv alira@sedici.unlp.edu.ar
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