Trichomonas vaginalis Exosomes Deliver Cargo to Host Cells and Mediate Host: Parasite Interactions

Trichomonas vaginalis is a common sexually transmitted parasite that colonizes the human urogential tract where it remains extracellular and adheres to epithelial cells. Infections range from asymptomatic to highly inflammatory, depending on the host and the parasite strain. Here, we use a combinati...

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Detalles Bibliográficos
Autores: Twu, Olivia, de Miguel, Natalia, Lustig, Gil, Stevens, Grant C., Vashisht, Ajay A., Wohlschlegel, James A., Johnson, Patricia J.
Tipo de recurso: artículo
Estado:Versión publicada
Fecha de publicación:2013
País:Argentina
Institución:Consejo Nacional de Investigaciones Científicas y Técnicas
Repositorio:CONICET Digital (CONICET)
Idioma:inglés
OAI Identifier:oai:ri.conicet.gov.ar:11336/24262
Acceso en línea:http://hdl.handle.net/11336/24262
Access Level:acceso abierto
Palabra clave:EXOSOMES
PARASITE
PATHOGENESIS
TRICHOMONAS
https://purl.org/becyt/ford/1.6
https://purl.org/becyt/ford/1
id AR_7af4fa8752de7a8ef8e75800ff522e19
oai_identifier_str oai:ri.conicet.gov.ar:11336/24262
network_acronym_str AR
network_name_str Argentina
repository_id_str
dc.title.none.fl_str_mv Trichomonas vaginalis Exosomes Deliver Cargo to Host Cells and Mediate Host: Parasite Interactions
title Trichomonas vaginalis Exosomes Deliver Cargo to Host Cells and Mediate Host: Parasite Interactions
spellingShingle Trichomonas vaginalis Exosomes Deliver Cargo to Host Cells and Mediate Host: Parasite Interactions
Twu, Olivia
EXOSOMES
PARASITE
PATHOGENESIS
TRICHOMONAS
https://purl.org/becyt/ford/1.6
https://purl.org/becyt/ford/1
title_short Trichomonas vaginalis Exosomes Deliver Cargo to Host Cells and Mediate Host: Parasite Interactions
title_full Trichomonas vaginalis Exosomes Deliver Cargo to Host Cells and Mediate Host: Parasite Interactions
title_fullStr Trichomonas vaginalis Exosomes Deliver Cargo to Host Cells and Mediate Host: Parasite Interactions
title_full_unstemmed Trichomonas vaginalis Exosomes Deliver Cargo to Host Cells and Mediate Host: Parasite Interactions
title_sort Trichomonas vaginalis Exosomes Deliver Cargo to Host Cells and Mediate Host: Parasite Interactions
dc.creator.none.fl_str_mv Twu, Olivia
de Miguel, Natalia
Lustig, Gil
Stevens, Grant C.
Vashisht, Ajay A.
Wohlschlegel, James A.
Johnson, Patricia J.
author Twu, Olivia
author_facet Twu, Olivia
de Miguel, Natalia
Lustig, Gil
Stevens, Grant C.
Vashisht, Ajay A.
Wohlschlegel, James A.
Johnson, Patricia J.
author_role author
author2 de Miguel, Natalia
Lustig, Gil
Stevens, Grant C.
Vashisht, Ajay A.
Wohlschlegel, James A.
Johnson, Patricia J.
author2_role author
author
author
author
author
author
dc.subject.none.fl_str_mv EXOSOMES
PARASITE
PATHOGENESIS
TRICHOMONAS
https://purl.org/becyt/ford/1.6
https://purl.org/becyt/ford/1
topic EXOSOMES
PARASITE
PATHOGENESIS
TRICHOMONAS
https://purl.org/becyt/ford/1.6
https://purl.org/becyt/ford/1
description Trichomonas vaginalis is a common sexually transmitted parasite that colonizes the human urogential tract where it remains extracellular and adheres to epithelial cells. Infections range from asymptomatic to highly inflammatory, depending on the host and the parasite strain. Here, we use a combination of methodologies including cell fractionation, immunofluorescence and electron microscopy, RNA, proteomic and cytokine analyses and cell adherence assays to examine pathogenic properties of T. vaginalis. We have found that T.vaginalis produces and secretes microvesicles with physical and biochemical properties similar to mammalian exosomes. The parasite-derived exosomes are characterized by the presence of RNA and core, conserved exosomal proteins as well as parasite-specific proteins. We demonstrate that T. vaginalis exosomes fuse with and deliver their contents to host cells and modulate host cell immune responses. Moreover, exosomes from highly adherent parasite strains increase the adherence of poorly adherent parasites to vaginal and prostate epithelial cells. In contrast, exosomes from poorly adherent strains had no measurable effect on parasite adherence. Exosomes from parasite strains that preferentially bind prostate cells increased binding of parasites to these cells relative to vaginal cells. In addition to establishing that parasite exosomes act to modulate host∶parasite interactions, these studies are the first to reveal a potential role for exosomes in promoting parasite∶parasite communication and host cell colonization.
publishDate 2013
dc.date.none.fl_str_mv 2013-07-11
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/24262
Twu, Olivia; de Miguel, Natalia; Lustig, Gil; Stevens, Grant C.; Vashisht, Ajay A.; et al.; Trichomonas vaginalis Exosomes Deliver Cargo to Host Cells and Mediate Host: Parasite Interactions; Public Library of Science; Plos Pathogens; 9; 7; 11-7-2013; 1-14
1553-7366
CONICET Digital
CONICET
url http://hdl.handle.net/11336/24262
identifier_str_mv Twu, Olivia; de Miguel, Natalia; Lustig, Gil; Stevens, Grant C.; Vashisht, Ajay A.; et al.; Trichomonas vaginalis Exosomes Deliver Cargo to Host Cells and Mediate Host: Parasite Interactions; Public Library of Science; Plos Pathogens; 9; 7; 11-7-2013; 1-14
1553-7366
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/doi/10.1371/journal.ppat.1003482
info:eu-repo/semantics/altIdentifier/url/http://journals.plos.org/plospathogens/article?id=10.1371/journal.ppat.1003482
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
dc.publisher.none.fl_str_mv Public Library of Science
publisher.none.fl_str_mv Public Library of Science
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
_version_ 1799196419198812160
spelling Trichomonas vaginalis Exosomes Deliver Cargo to Host Cells and Mediate Host: Parasite InteractionsTwu, Oliviade Miguel, NataliaLustig, GilStevens, Grant C.Vashisht, Ajay A.Wohlschlegel, James A.Johnson, Patricia J.EXOSOMESPARASITEPATHOGENESISTRICHOMONAShttps://purl.org/becyt/ford/1.6https://purl.org/becyt/ford/1Trichomonas vaginalis is a common sexually transmitted parasite that colonizes the human urogential tract where it remains extracellular and adheres to epithelial cells. Infections range from asymptomatic to highly inflammatory, depending on the host and the parasite strain. Here, we use a combination of methodologies including cell fractionation, immunofluorescence and electron microscopy, RNA, proteomic and cytokine analyses and cell adherence assays to examine pathogenic properties of T. vaginalis. We have found that T.vaginalis produces and secretes microvesicles with physical and biochemical properties similar to mammalian exosomes. The parasite-derived exosomes are characterized by the presence of RNA and core, conserved exosomal proteins as well as parasite-specific proteins. We demonstrate that T. vaginalis exosomes fuse with and deliver their contents to host cells and modulate host cell immune responses. Moreover, exosomes from highly adherent parasite strains increase the adherence of poorly adherent parasites to vaginal and prostate epithelial cells. In contrast, exosomes from poorly adherent strains had no measurable effect on parasite adherence. Exosomes from parasite strains that preferentially bind prostate cells increased binding of parasites to these cells relative to vaginal cells. In addition to establishing that parasite exosomes act to modulate host∶parasite interactions, these studies are the first to reveal a potential role for exosomes in promoting parasite∶parasite communication and host cell colonization.Fil: Twu, Olivia. University of California at Los Angeles; Estados UnidosFil: de Miguel, Natalia. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata. Instituto de Investigaciones Biotecnológicas. Instituto de Investigaciones Biotecnológicas "Dr. Raúl Alfonsín" (sede Chascomús). Universidad Nacional de San Martín. Instituto de Investigaciones Biotecnológicas. Instituto de Investigaciones Biotecnológicas "Dr. Raúl Alfonsín" (sede Chascomús); Argentina. University of California at Los Angeles; Estados UnidosFil: Lustig, Gil. University of California at Los Angeles; Estados UnidosFil: Stevens, Grant C.. University of California at Los Angeles; Estados UnidosFil: Vashisht, Ajay A.. University of California at Los Angeles; Estados UnidosFil: Wohlschlegel, James A.. University of California at Los Angeles; Estados UnidosFil: Johnson, Patricia J.. University of California at Los Angeles; Estados UnidosPublic Library of Science2013-07-11info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/24262Twu, Olivia; de Miguel, Natalia; Lustig, Gil; Stevens, Grant C.; Vashisht, Ajay A.; et al.; Trichomonas vaginalis Exosomes Deliver Cargo to Host Cells and Mediate Host: Parasite Interactions; Public Library of Science; Plos Pathogens; 9; 7; 11-7-2013; 1-141553-7366CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/doi/10.1371/journal.ppat.1003482info:eu-repo/semantics/altIdentifier/url/http://journals.plos.org/plospathogens/article?id=10.1371/journal.ppat.1003482info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2024-05-08T14:26:56Zoai:ri.conicet.gov.ar:11336/24262instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982024-05-08 14:26:56.906CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
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