High-throughput characterization of Echinococcus spp. metacestode miRNomes

Echinococcosis is a worldwide zoonosis of great public health concern, considered a neglected disease by the World Health Organisation. The cestode parasites Echinococcus granulosus sensu lato (s. l.) and Echinococcus multilocularis are the main aetiological agents. In the intermediate host, these p...

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Detalhes bibliográficos
Autores: Cucher, Marcela Alejandra, Macchiaroli, Natalia, Kamenetzky, Laura, Maldonado, Lucas Luciano, Brehm, Klaus, Rosenzvit, Mara Cecilia
Formato: artículo
Estado:Versión publicada
Fecha de publicación:2015
País:Argentina
Recursos:Consejo Nacional de Investigaciones Científicas y Técnicas
Repositorio:CONICET Digital (CONICET)
Idioma:inglés
OAI Identifier:oai:ri.conicet.gov.ar:11336/38915
Acesso em linha:http://hdl.handle.net/11336/38915
Access Level:acceso abierto
Palavra-chave:Echinococcosis
Echinococcus
Microrna
Parasite
Platyhelminth
Small Rna
Srna-Seq
https://purl.org/becyt/ford/3.3
https://purl.org/becyt/ford/3
id AR_7aee9852404fcb73b0fbddf10aada7ad
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network_acronym_str AR
network_name_str Argentina
repository_id_str
dc.title.none.fl_str_mv High-throughput characterization of Echinococcus spp. metacestode miRNomes
title High-throughput characterization of Echinococcus spp. metacestode miRNomes
spellingShingle High-throughput characterization of Echinococcus spp. metacestode miRNomes
Cucher, Marcela Alejandra
Echinococcosis
Echinococcus
Microrna
Parasite
Platyhelminth
Small Rna
Srna-Seq
https://purl.org/becyt/ford/3.3
https://purl.org/becyt/ford/3
title_short High-throughput characterization of Echinococcus spp. metacestode miRNomes
title_full High-throughput characterization of Echinococcus spp. metacestode miRNomes
title_fullStr High-throughput characterization of Echinococcus spp. metacestode miRNomes
title_full_unstemmed High-throughput characterization of Echinococcus spp. metacestode miRNomes
title_sort High-throughput characterization of Echinococcus spp. metacestode miRNomes
dc.creator.none.fl_str_mv Cucher, Marcela Alejandra
Macchiaroli, Natalia
Kamenetzky, Laura
Maldonado, Lucas Luciano
Brehm, Klaus
Rosenzvit, Mara Cecilia
author Cucher, Marcela Alejandra
author_facet Cucher, Marcela Alejandra
Macchiaroli, Natalia
Kamenetzky, Laura
Maldonado, Lucas Luciano
Brehm, Klaus
Rosenzvit, Mara Cecilia
author_role author
author2 Macchiaroli, Natalia
Kamenetzky, Laura
Maldonado, Lucas Luciano
Brehm, Klaus
Rosenzvit, Mara Cecilia
author2_role author
author
author
author
author
dc.subject.none.fl_str_mv Echinococcosis
Echinococcus
Microrna
Parasite
Platyhelminth
Small Rna
Srna-Seq
https://purl.org/becyt/ford/3.3
https://purl.org/becyt/ford/3
topic Echinococcosis
Echinococcus
Microrna
Parasite
Platyhelminth
Small Rna
Srna-Seq
https://purl.org/becyt/ford/3.3
https://purl.org/becyt/ford/3
description Echinococcosis is a worldwide zoonosis of great public health concern, considered a neglected disease by the World Health Organisation. The cestode parasites Echinococcus granulosus sensu lato (s. l.) and Echinococcus multilocularis are the main aetiological agents. In the intermediate host, these parasites display particular developmental traits that lead to different patterns of disease progression. In an attempt to understand the causes of these differences, we focused on the analysis of microRNAs (miRNAs), small non-coding regulatory RNAs with major roles in development of animals and plants. In this work, we analysed the small RNA expression pattern of the metacestode, the stage of sanitary relevance, and provide a detailed description of Echinococcus miRNAs. Using high-throughput small RNA sequencing, we believe that we have carried out the first experimental identification of miRNAs in E. multilocularis and have expanded the Echinococcus miRNA catalogue to 38 miRNA genes, including one miRNA only present in E. granulosus s. l. Our findings show that although both species share the top five highest expressed miRNAs, 13 are differentially expressed, which could be related to developmental differences. We also provide evidence that uridylation is the main miRNA processing mechanism in Echinococcus spp. These results provide detailed information on Echinococcus miRNAs, which is the first step in understanding their role in parasite biology and disease establishment and/or progression, and their future potential use as drug or diagnostic targets.
publishDate 2015
dc.date.none.fl_str_mv 2015-03
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/38915
Cucher, Marcela Alejandra; Macchiaroli, Natalia; Kamenetzky, Laura; Maldonado, Lucas Luciano; Brehm, Klaus; et al.; High-throughput characterization of Echinococcus spp. metacestode miRNomes; Elsevier; International Journal for Parasitology; 45; 4; 3-2015; 253-267
0020-7519
CONICET Digital
CONICET
url http://hdl.handle.net/11336/38915
identifier_str_mv Cucher, Marcela Alejandra; Macchiaroli, Natalia; Kamenetzky, Laura; Maldonado, Lucas Luciano; Brehm, Klaus; et al.; High-throughput characterization of Echinococcus spp. metacestode miRNomes; Elsevier; International Journal for Parasitology; 45; 4; 3-2015; 253-267
0020-7519
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/doi/10.1016/j.ijpara.2014.12.003
info:eu-repo/semantics/altIdentifier/url/https://www.sciencedirect.com/science/article/pii/S002075191500017X
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-nd/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-nd/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
application/pdf
application/pdf
application/pdf
application/pdf
dc.publisher.none.fl_str_mv Elsevier
publisher.none.fl_str_mv Elsevier
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
_version_ 1799195583019220992
spelling High-throughput characterization of Echinococcus spp. metacestode miRNomesCucher, Marcela AlejandraMacchiaroli, NataliaKamenetzky, LauraMaldonado, Lucas LucianoBrehm, KlausRosenzvit, Mara CeciliaEchinococcosisEchinococcusMicrornaParasitePlatyhelminthSmall RnaSrna-Seqhttps://purl.org/becyt/ford/3.3https://purl.org/becyt/ford/3Echinococcosis is a worldwide zoonosis of great public health concern, considered a neglected disease by the World Health Organisation. The cestode parasites Echinococcus granulosus sensu lato (s. l.) and Echinococcus multilocularis are the main aetiological agents. In the intermediate host, these parasites display particular developmental traits that lead to different patterns of disease progression. In an attempt to understand the causes of these differences, we focused on the analysis of microRNAs (miRNAs), small non-coding regulatory RNAs with major roles in development of animals and plants. In this work, we analysed the small RNA expression pattern of the metacestode, the stage of sanitary relevance, and provide a detailed description of Echinococcus miRNAs. Using high-throughput small RNA sequencing, we believe that we have carried out the first experimental identification of miRNAs in E. multilocularis and have expanded the Echinococcus miRNA catalogue to 38 miRNA genes, including one miRNA only present in E. granulosus s. l. Our findings show that although both species share the top five highest expressed miRNAs, 13 are differentially expressed, which could be related to developmental differences. We also provide evidence that uridylation is the main miRNA processing mechanism in Echinococcus spp. These results provide detailed information on Echinococcus miRNAs, which is the first step in understanding their role in parasite biology and disease establishment and/or progression, and their future potential use as drug or diagnostic targets.Fil: Cucher, Marcela Alejandra. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay. Instituto de Investigaciones en Microbiología y Parasitología Médica. Universidad de Buenos Aires. Facultad de Medicina. Instituto de Investigaciones en Microbiología y Parasitología Médica; ArgentinaFil: Macchiaroli, Natalia. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay. Instituto de Investigaciones en Microbiología y Parasitología Médica. Universidad de Buenos Aires. Facultad de Medicina. Instituto de Investigaciones en Microbiología y Parasitología Médica; ArgentinaFil: Kamenetzky, Laura. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay. Instituto de Investigaciones en Microbiología y Parasitología Médica. Universidad de Buenos Aires. Facultad de Medicina. Instituto de Investigaciones en Microbiología y Parasitología Médica; ArgentinaFil: Maldonado, Lucas Luciano. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay. Instituto de Investigaciones en Microbiología y Parasitología Médica. Universidad de Buenos Aires. Facultad de Medicina. Instituto de Investigaciones en Microbiología y Parasitología Médica; ArgentinaFil: Brehm, Klaus. Universität Würzburg; AlemaniaFil: Rosenzvit, Mara Cecilia. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay. Instituto de Investigaciones en Microbiología y Parasitología Médica. Universidad de Buenos Aires. Facultad de Medicina. Instituto de Investigaciones en Microbiología y Parasitología Médica; ArgentinaElsevier2015-03info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfapplication/pdfapplication/pdfapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/38915Cucher, Marcela Alejandra; Macchiaroli, Natalia; Kamenetzky, Laura; Maldonado, Lucas Luciano; Brehm, Klaus; et al.; High-throughput characterization of Echinococcus spp. metacestode miRNomes; Elsevier; International Journal for Parasitology; 45; 4; 3-2015; 253-2670020-7519CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/doi/10.1016/j.ijpara.2014.12.003info:eu-repo/semantics/altIdentifier/url/https://www.sciencedirect.com/science/article/pii/S002075191500017Xinfo:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-nd/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2024-05-08T13:57:05Zoai:ri.conicet.gov.ar:11336/38915instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982024-05-08 13:57:06.01CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
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