One-step concentration and partial purification of <i>Aspergillus kawachii</i> non-acidic polygalacturonases by adsorption to glass fiber microfilters
The non-acidic polygalacturonases produced by <i>Aspergillus kawachii</i> in a glucose/tryptone medium were adsorbed to a glass fiber microfilter that was used to clarify the fermentation broth. Maximum adsorption occurred at pH 3 under low ionic strength conditions. The adsorbed activit...
| Autores: | , , |
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| Formato: | artículo |
| Estado: | Versión publicada |
| Fecha de publicación: | 2006 |
| País: | Argentina |
| Recursos: | Universidad Nacional de La Plata |
| Repositorio: | SEDICI (UNLP) |
| Idioma: | inglés |
| OAI Identifier: | oai:sedici.unlp.edu.ar:10915/145008 |
| Acesso em linha: | http://sedici.unlp.edu.ar/handle/10915/145008 |
| Access Level: | acceso abierto |
| Palavra-chave: | Ciencias Exactas Química Aspergillus kawachii glass fiber microfilter polygalacturonases protein adsorption |
| Resumo: | The non-acidic polygalacturonases produced by <i>Aspergillus kawachii</i> in a glucose/tryptone medium were adsorbed to a glass fiber microfilter that was used to clarify the fermentation broth. Maximum adsorption occurred at pH 3 under low ionic strength conditions. The adsorbed activity could be readily released with a buffer solution at pH 5. Based upon these observations, a separation process was developed which enabled the broth to be clarified and, at the same time, the non-acidic polygalacturonases to be concentrated 20-fold and purified 100-fold in a unique filtration step. The practical advantage of recovering polygalacturonases by a filtration process lies in the simplicity and efficiency of the operation involved. |
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