Solid surface fluorescence methodology for fast monitoring of 2,4-dichlorophenoxyacetic acid in seed samples
A new method for pre-concentration/separation and determination of 2,4-dichlorophenoxyacetic acid (2,4-D) by solid-surface fluorescence (SSF) is proposed. The herbicide was complexed with Rhodamine B at pH 7.0 in the presence of phosphate buffer and anionic surfactant admicelles. A Nylon membrane wa...
| Autores: | , , |
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| Tipo de recurso: | artículo |
| Estado: | Versión publicada |
| Fecha de publicación: | 2017 |
| País: | Argentina |
| Institución: | Consejo Nacional de Investigaciones Científicas y Técnicas |
| Repositorio: | CONICET Digital (CONICET) |
| Idioma: | inglés |
| OAI Identifier: | oai:ri.conicet.gov.ar:11336/64767 |
| Acceso en línea: | http://hdl.handle.net/11336/64767 |
| Access Level: | acceso abierto |
| Palabra clave: | Herbicide Traces 2,4-Dichlorophenoxyacetic Acid Rhodamine B Solid Surface Fluorescence Seed Samples https://purl.org/becyt/ford/1.4 https://purl.org/becyt/ford/1 |
| Sumario: | A new method for pre-concentration/separation and determination of 2,4-dichlorophenoxyacetic acid (2,4-D) by solid-surface fluorescence (SSF) is proposed. The herbicide was complexed with Rhodamine B at pH 7.0 in the presence of phosphate buffer and anionic surfactant admicelles. A Nylon membrane was selected as a solid support for SSF measurement and the presence of 2,4-D was evident by the RhB quenching effect. Under optimal experimental conditions, the limit of detection and quantification were 6.93 and 21 ng L−1 , respectively, and the linear range was obtained from 0.021 to 22.11 μg L−1 2,4-D concentration. The developed methodology showed good sensitivity and adequate selectivity, and it was applied to the 2,4-D determination in seed samples. The SSF represents a simple and fast alternative to conventional methods of analysis, employing an existing instrument in most laboratories. |
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