Phospholipid turnover and phospholipase D activity in tobacco hairy roots exposed to phenol

Plants are exposed to a great variety of environmental factors, which can affect their survival. In addition, the rapid urbanization and the increased release of different pollutants, such as phenol, to the environment, produce another stressful condition to the development and growth of the plants....

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Autores: Sosa Alderete, Lucas Gastón, Racagni, Graciela Esther, Agostini, Elizabeth, Medina, María I.
Tipo de recurso: artículo
Estado:Versión publicada
Fecha de publicación:2012
País:Argentina
Institución:Consejo Nacional de Investigaciones Científicas y Técnicas
Repositorio:CONICET Digital (CONICET)
Idioma:inglés
OAI Identifier:oai:ri.conicet.gov.ar:11336/230401
Acceso en línea:http://hdl.handle.net/11336/230401
Access Level:acceso abierto
Palabra clave:PHENOL
PLD
PHOSPHOLIPIDS
SIGNAL TRANSDUCTION
https://purl.org/becyt/ford/2.8
https://purl.org/becyt/ford/2
id AR_776ebc9762dc2c8a36e5edc4bb6de246
oai_identifier_str oai:ri.conicet.gov.ar:11336/230401
network_acronym_str AR
network_name_str Argentina
repository_id_str
dc.title.none.fl_str_mv Phospholipid turnover and phospholipase D activity in tobacco hairy roots exposed to phenol
title Phospholipid turnover and phospholipase D activity in tobacco hairy roots exposed to phenol
spellingShingle Phospholipid turnover and phospholipase D activity in tobacco hairy roots exposed to phenol
Sosa Alderete, Lucas Gastón
PHENOL
PLD
PHOSPHOLIPIDS
SIGNAL TRANSDUCTION
https://purl.org/becyt/ford/2.8
https://purl.org/becyt/ford/2
title_short Phospholipid turnover and phospholipase D activity in tobacco hairy roots exposed to phenol
title_full Phospholipid turnover and phospholipase D activity in tobacco hairy roots exposed to phenol
title_fullStr Phospholipid turnover and phospholipase D activity in tobacco hairy roots exposed to phenol
title_full_unstemmed Phospholipid turnover and phospholipase D activity in tobacco hairy roots exposed to phenol
title_sort Phospholipid turnover and phospholipase D activity in tobacco hairy roots exposed to phenol
dc.creator.none.fl_str_mv Sosa Alderete, Lucas Gastón
Racagni, Graciela Esther
Agostini, Elizabeth
Medina, María I.
author Sosa Alderete, Lucas Gastón
author_facet Sosa Alderete, Lucas Gastón
Racagni, Graciela Esther
Agostini, Elizabeth
Medina, María I.
author_role author
author2 Racagni, Graciela Esther
Agostini, Elizabeth
Medina, María I.
author2_role author
author
author
dc.subject.none.fl_str_mv PHENOL
PLD
PHOSPHOLIPIDS
SIGNAL TRANSDUCTION
https://purl.org/becyt/ford/2.8
https://purl.org/becyt/ford/2
topic PHENOL
PLD
PHOSPHOLIPIDS
SIGNAL TRANSDUCTION
https://purl.org/becyt/ford/2.8
https://purl.org/becyt/ford/2
description Plants are exposed to a great variety of environmental factors, which can affect their survival. In addition, the rapid urbanization and the increased release of different pollutants, such as phenol, to the environment, produce another stressful condition to the development and growth of the plants. In this work, we studied the effects on the [32P]Piphospholipid turnover and phospholipase D (PLD) activity after phenol treatment, using tobacco hairy roots (HRs), double transgenic (DT) for two peroxidase genes (tpx1 and tpx2) and wild type (WT) ones. In both HRs, the [32P]phospholipid turnover of the most abundant phospholipids (PLs), such as phosphatidylcholine (PC), phosphatydilethanolamine (PE), phosphatydilglycerol (PG) and cardiolipin (CL) did not show any changes after phenol treatment. However, modifications in the minor PLs of both HRs were observed. Phenol treatment significantly increases the turnover of phosphatidic acid (PA) and phosphatydilinositol (PI), in WT HRs. In DT HRs, phenol produced significant increase in the turnover of PI, lisophosphatidic acid (LPA), diacylglycerolpyrophosphate (DGPP) and PA with a concomitant decrease in the phosphatidylinositol monophosphate (PIP). Moreover, phosphatidylinositol bisphosphate (PIP2) was detected, but its level did not change in presence of the pollutant. Phenol treatment significantly increased the PLD activity of both HRs. In WT HRs the increase was 100% higher than the control, whereas in DT HRs it was about to 50%. These results suggest the participation of minor PLs, mainly PA, and the PLD pathway as one source of PA production in the activation of intracellular mechanisms that might be important in the response of these plant tissues to phenol treatment.32P]Piphospholipid turnover and phospholipase D (PLD) activity after phenol treatment, using tobacco hairy roots (HRs), double transgenic (DT) for two peroxidase genes (tpx1 and tpx2) and wild type (WT) ones. In both HRs, the [32P]phospholipid turnover of the most abundant phospholipids (PLs), such as phosphatidylcholine (PC), phosphatydilethanolamine (PE), phosphatydilglycerol (PG) and cardiolipin (CL) did not show any changes after phenol treatment. However, modifications in the minor PLs of both HRs were observed. Phenol treatment significantly increases the turnover of phosphatidic acid (PA) and phosphatydilinositol (PI), in WT HRs. In DT HRs, phenol produced significant increase in the turnover of PI, lisophosphatidic acid (LPA), diacylglycerolpyrophosphate (DGPP) and PA with a concomitant decrease in the phosphatidylinositol monophosphate (PIP). Moreover, phosphatidylinositol bisphosphate (PIP2) was detected, but its level did not change in presence of the pollutant. Phenol treatment significantly increased the PLD activity of both HRs. In WT HRs the increase was 100% higher than the control, whereas in DT HRs it was about to 50%. These results suggest the participation of minor PLs, mainly PA, and the PLD pathway as one source of PA production in the activation of intracellular mechanisms that might be important in the response of these plant tissues to phenol treatment.32P]phospholipid turnover of the most abundant phospholipids (PLs), such as phosphatidylcholine (PC), phosphatydilethanolamine (PE), phosphatydilglycerol (PG) and cardiolipin (CL) did not show any changes after phenol treatment. However, modifications in the minor PLs of both HRs were observed. Phenol treatment significantly increases the turnover of phosphatidic acid (PA) and phosphatydilinositol (PI), in WT HRs. In DT HRs, phenol produced significant increase in the turnover of PI, lisophosphatidic acid (LPA), diacylglycerolpyrophosphate (DGPP) and PA with a concomitant decrease in the phosphatidylinositol monophosphate (PIP). Moreover, phosphatidylinositol bisphosphate (PIP2) was detected, but its level did not change in presence of the pollutant. Phenol treatment significantly increased the PLD activity of both HRs. In WT HRs the increase was 100% higher than the control, whereas in DT HRs it was about to 50%. These results suggest the participation of minor PLs, mainly PA, and the PLD pathway as one source of PA production in the activation of intracellular mechanisms that might be important in the response of these plant tissues to phenol treatment.2) was detected, but its level did not change in presence of the pollutant. Phenol treatment significantly increased the PLD activity of both HRs. In WT HRs the increase was 100% higher than the control, whereas in DT HRs it was about to 50%. These results suggest the participation of minor PLs, mainly PA, and the PLD pathway as one source of PA production in the activation of intracellular mechanisms that might be important in the response of these plant tissues to phenol treatment.
publishDate 2012
dc.date.none.fl_str_mv 2012-04
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/230401
Sosa Alderete, Lucas Gastón; Racagni, Graciela Esther; Agostini, Elizabeth; Medina, María I.; Phospholipid turnover and phospholipase D activity in tobacco hairy roots exposed to phenol; Pergamon-Elsevier Science Ltd; Environmental and Experimental Botany; 77; 4-2012; 141-145
0098-8472
1873-7307
CONICET Digital
CONICET
url http://hdl.handle.net/11336/230401
identifier_str_mv Sosa Alderete, Lucas Gastón; Racagni, Graciela Esther; Agostini, Elizabeth; Medina, María I.; Phospholipid turnover and phospholipase D activity in tobacco hairy roots exposed to phenol; Pergamon-Elsevier Science Ltd; Environmental and Experimental Botany; 77; 4-2012; 141-145
0098-8472
1873-7307
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/doi/10.1016/j.envexpbot.2011.11.006
info:eu-repo/semantics/altIdentifier/url/https://www.sciencedirect.com/science/article/pii/S0098847211002826
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
application/pdf
application/pdf
application/pdf
dc.publisher.none.fl_str_mv Pergamon-Elsevier Science Ltd
publisher.none.fl_str_mv Pergamon-Elsevier Science Ltd
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
_version_ 1799195586137686016
spelling Phospholipid turnover and phospholipase D activity in tobacco hairy roots exposed to phenolSosa Alderete, Lucas GastónRacagni, Graciela EstherAgostini, ElizabethMedina, María I.PHENOLPLDPHOSPHOLIPIDSSIGNAL TRANSDUCTIONhttps://purl.org/becyt/ford/2.8https://purl.org/becyt/ford/2Plants are exposed to a great variety of environmental factors, which can affect their survival. In addition, the rapid urbanization and the increased release of different pollutants, such as phenol, to the environment, produce another stressful condition to the development and growth of the plants. In this work, we studied the effects on the [32P]Piphospholipid turnover and phospholipase D (PLD) activity after phenol treatment, using tobacco hairy roots (HRs), double transgenic (DT) for two peroxidase genes (tpx1 and tpx2) and wild type (WT) ones. In both HRs, the [32P]phospholipid turnover of the most abundant phospholipids (PLs), such as phosphatidylcholine (PC), phosphatydilethanolamine (PE), phosphatydilglycerol (PG) and cardiolipin (CL) did not show any changes after phenol treatment. However, modifications in the minor PLs of both HRs were observed. Phenol treatment significantly increases the turnover of phosphatidic acid (PA) and phosphatydilinositol (PI), in WT HRs. In DT HRs, phenol produced significant increase in the turnover of PI, lisophosphatidic acid (LPA), diacylglycerolpyrophosphate (DGPP) and PA with a concomitant decrease in the phosphatidylinositol monophosphate (PIP). Moreover, phosphatidylinositol bisphosphate (PIP2) was detected, but its level did not change in presence of the pollutant. Phenol treatment significantly increased the PLD activity of both HRs. In WT HRs the increase was 100% higher than the control, whereas in DT HRs it was about to 50%. These results suggest the participation of minor PLs, mainly PA, and the PLD pathway as one source of PA production in the activation of intracellular mechanisms that might be important in the response of these plant tissues to phenol treatment.32P]Piphospholipid turnover and phospholipase D (PLD) activity after phenol treatment, using tobacco hairy roots (HRs), double transgenic (DT) for two peroxidase genes (tpx1 and tpx2) and wild type (WT) ones. In both HRs, the [32P]phospholipid turnover of the most abundant phospholipids (PLs), such as phosphatidylcholine (PC), phosphatydilethanolamine (PE), phosphatydilglycerol (PG) and cardiolipin (CL) did not show any changes after phenol treatment. However, modifications in the minor PLs of both HRs were observed. Phenol treatment significantly increases the turnover of phosphatidic acid (PA) and phosphatydilinositol (PI), in WT HRs. In DT HRs, phenol produced significant increase in the turnover of PI, lisophosphatidic acid (LPA), diacylglycerolpyrophosphate (DGPP) and PA with a concomitant decrease in the phosphatidylinositol monophosphate (PIP). Moreover, phosphatidylinositol bisphosphate (PIP2) was detected, but its level did not change in presence of the pollutant. Phenol treatment significantly increased the PLD activity of both HRs. In WT HRs the increase was 100% higher than the control, whereas in DT HRs it was about to 50%. These results suggest the participation of minor PLs, mainly PA, and the PLD pathway as one source of PA production in the activation of intracellular mechanisms that might be important in the response of these plant tissues to phenol treatment.32P]phospholipid turnover of the most abundant phospholipids (PLs), such as phosphatidylcholine (PC), phosphatydilethanolamine (PE), phosphatydilglycerol (PG) and cardiolipin (CL) did not show any changes after phenol treatment. However, modifications in the minor PLs of both HRs were observed. Phenol treatment significantly increases the turnover of phosphatidic acid (PA) and phosphatydilinositol (PI), in WT HRs. In DT HRs, phenol produced significant increase in the turnover of PI, lisophosphatidic acid (LPA), diacylglycerolpyrophosphate (DGPP) and PA with a concomitant decrease in the phosphatidylinositol monophosphate (PIP). Moreover, phosphatidylinositol bisphosphate (PIP2) was detected, but its level did not change in presence of the pollutant. Phenol treatment significantly increased the PLD activity of both HRs. In WT HRs the increase was 100% higher than the control, whereas in DT HRs it was about to 50%. These results suggest the participation of minor PLs, mainly PA, and the PLD pathway as one source of PA production in the activation of intracellular mechanisms that might be important in the response of these plant tissues to phenol treatment.2) was detected, but its level did not change in presence of the pollutant. Phenol treatment significantly increased the PLD activity of both HRs. In WT HRs the increase was 100% higher than the control, whereas in DT HRs it was about to 50%. These results suggest the participation of minor PLs, mainly PA, and the PLD pathway as one source of PA production in the activation of intracellular mechanisms that might be important in the response of these plant tissues to phenol treatment.Fil: Sosa Alderete, Lucas Gastón. Universidad Nacional de Río Cuarto. Facultad de Ciencias Exactas Fisicoquímicas y Naturales. Instituto de Biotecnología Ambiental y Salud - Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba. Instituto de Biotecnología Ambiental y Salud; ArgentinaFil: Racagni, Graciela Esther. Universidad Nacional de Río Cuarto. Facultad de Ciencias Exactas Fisicoquímicas y Naturales; ArgentinaFil: Agostini, Elizabeth. Universidad Nacional de Río Cuarto. Facultad de Ciencias Exactas Fisicoquímicas y Naturales. Instituto de Biotecnología Ambiental y Salud - Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba. Instituto de Biotecnología Ambiental y Salud; ArgentinaFil: Medina, María I.. Universidad Nacional de Río Cuarto. Facultad de Ciencias Exactas Fisicoquímicas y Naturales; ArgentinaPergamon-Elsevier Science Ltd2012-04info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfapplication/pdfapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/230401Sosa Alderete, Lucas Gastón; Racagni, Graciela Esther; Agostini, Elizabeth; Medina, María I.; Phospholipid turnover and phospholipase D activity in tobacco hairy roots exposed to phenol; Pergamon-Elsevier Science Ltd; Environmental and Experimental Botany; 77; 4-2012; 141-1450098-84721873-7307CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/doi/10.1016/j.envexpbot.2011.11.006info:eu-repo/semantics/altIdentifier/url/https://www.sciencedirect.com/science/article/pii/S0098847211002826info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2024-05-08T13:57:09Zoai:ri.conicet.gov.ar:11336/230401instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982024-05-08 13:57:09.542CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
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