Determination of hydrodynamic properties of bovine serum albumin (BSA)

This work allows combining theoretical fundamentals along with the development of abilities and skills on applied hydrodynamics. This is a complementary tool used to establish or to confirm the structures and interactions of biomolecules, especially in diluted solutions. In this laboratory, viscosit...

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Detalles Bibliográficos
Autores: Curvale, Rolando, Cesco, Juan Carlos, Debatista, Nora B, Pappano, Nora B.
Tipo de recurso: artículo
Estado:Versión publicada
Fecha de publicación:2010
País:Argentina
Institución:Consejo Nacional de Investigaciones Científicas y Técnicas
Repositorio:CONICET Digital (CONICET)
Idioma:inglés
OAI Identifier:oai:ri.conicet.gov.ar:11336/15638
Acceso en línea:http://hdl.handle.net/11336/15638
Access Level:acceso abierto
Palabra clave:Laboratories And Demonstrations
Physical Chemistry
Analytical Chemistry
Hydrodynamic Properties
Bovine Serum Albumin
https://purl.org/becyt/ford/1.4
https://purl.org/becyt/ford/1
Descripción
Sumario:This work allows combining theoretical fundamentals along with the development of abilities and skills on applied hydrodynamics. This is a complementary tool used to establish or to confirm the structures and interactions of biomolecules, especially in diluted solutions. In this laboratory, viscosity measurements of bovine serum albumin solutions at different pH values are used to obtain parameters related to form and size of BSA conformers. This practice, which can be performed in three hours, presents the advantage of using low cost reagents and equipment common to many laboratories, which in turn facilitates student participation in their process of learning.