β-xylosidase activity and expression of a β-xylosidase gene during strawberry fruit ripening
Strawberry fruit shows a marked softening during ripening and the process is associated with an increment of pectin solubility and a reduction of the molecular mass of hemicelluloses. In this work, we report the activity of β-xylosidase and the expression of a β-xylosidase gene in strawberry fruit....
| Autores: | , , |
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| Tipo de recurso: | artículo |
| Estado: | Versión publicada |
| Fecha de publicación: | 2004 |
| País: | Argentina |
| Institución: | Consejo Nacional de Investigaciones Científicas y Técnicas |
| Repositorio: | CONICET Digital (CONICET) |
| Idioma: | inglés |
| OAI Identifier: | oai:ri.conicet.gov.ar:11336/148747 |
| Acceso en línea: | http://hdl.handle.net/11336/148747 |
| Access Level: | acceso abierto |
| Palabra clave: | FRUIT RIPENING STRAWBERRY XYLOSIDASE https://purl.org/becyt/ford/2.11 https://purl.org/becyt/ford/2 |
| Sumario: | Strawberry fruit shows a marked softening during ripening and the process is associated with an increment of pectin solubility and a reduction of the molecular mass of hemicelluloses. In this work, we report the activity of β-xylosidase and the expression of a β-xylosidase gene in strawberry fruit. We have cloned a cDNA fragment encoding a putative β-xylosidase (FaXyl1) from a cDNA library obtained from ripe strawberry fruit. The analysis of the deduced amino acid sequence revealed that FaXyl1 is closely related to other β-xylosidases from higher plants. The expression of FaXyl1 was strongly associated to the receptacle tissue although a low expression level was detected in achenes and ovaries. The accumulation of FaXyl1 mRNA is ripening-related, starting in white fruit, reaching the maximum at 25–50% red fruit and decreasing thereafter. The total β-xylosidase enzyme activity was detected in all ripening stages with the maximum in 25–50% red fruit. The low activity level detected in immature stages, where no expression of FaXyl1 was found, suggests the presence of other β-xylosidases-like genes. Both the expression of FaXyl1 and the total β-xylosidase activity were down regulated by auxins, as occurs for most of the ripening-related processes in strawberry fruit. A putative role of FaXyl1 and β-xylosidase is discussed. |
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