Synchronous fluorescence spectrometry: Conformational investigation or inner filter effect?

Synchronous fluorescence spectrometry has become a common tool to investigate conformational changes in proteins due to the presence of other molecules (quenchers). The same pattern is always followed, leaving aside the possibility of interfering effects that make conclusions incorrect. In this pape...

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Detalles Bibliográficos
Autores: Pacheco, Maria Emilia, Bruzzone, Liliana
Tipo de recurso: artículo
Estado:Versión publicada
Fecha de publicación:2013
País:Argentina
Institución:Consejo Nacional de Investigaciones Científicas y Técnicas
Repositorio:CONICET Digital (CONICET)
Idioma:inglés
OAI Identifier:oai:ri.conicet.gov.ar:11336/22862
Acceso en línea:http://hdl.handle.net/11336/22862
Access Level:acceso abierto
Palabra clave:Synchronous Fluorescence Spectrometry
Inner Filter Effect
Bovine Serum Albumin
Imazethapyr
Chlorimuron-Ethyl
https://purl.org/becyt/ford/1.4
https://purl.org/becyt/ford/1
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spelling Synchronous fluorescence spectrometry: Conformational investigation or inner filter effect?Pacheco, Maria EmiliaBruzzone, LilianaSynchronous Fluorescence SpectrometryInner Filter EffectBovine Serum AlbuminImazethapyrChlorimuron-Ethylhttps://purl.org/becyt/ford/1.4https://purl.org/becyt/ford/1Synchronous fluorescence spectrometry has become a common tool to investigate conformational changes in proteins due to the presence of other molecules (quenchers). The same pattern is always followed, leaving aside the possibility of interfering effects that make conclusions incorrect. In this paper, we discuss the main cause of a wrong understanding of synchronous fluorescence spectrometry in a large number of papers published in many different journals. Based on the principles of synchronous fluorescence spectrometry, we perform a simple correction method of synchronous spectra taking into account inner filter effects. This criticism tries to demonstrate that the interpretation of synchronous fluorescence spectrometry is not as easy as it seems.Fil: Pacheco, Maria Emilia. Universidad Nacional de la Plata. Facultad de Ciencias Exactas. Departamento de Quimica; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaFil: Bruzzone, Liliana. Universidad Nacional de la Plata. Facultad de Ciencias Exactas. Departamento de Quimica; ArgentinaElsevier Science2013-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/22862Pacheco, Maria Emilia; Bruzzone, Liliana; Synchronous fluorescence spectrometry: Conformational investigation or inner filter effect?; Elsevier Science; Journal of Luminescence; 137; 1-2013; 138-1420022-2313CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/doi/10.1016/j.jlumin.2012.12.056info:eu-repo/semantics/altIdentifier/url/http://www.sciencedirect.com/science/article/pii/S0022231313000021info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-nd/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2024-05-08T13:51:11Zoai:ri.conicet.gov.ar:11336/22862instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982024-05-08 13:51:11.689CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Synchronous fluorescence spectrometry: Conformational investigation or inner filter effect?
title Synchronous fluorescence spectrometry: Conformational investigation or inner filter effect?
spellingShingle Synchronous fluorescence spectrometry: Conformational investigation or inner filter effect?
Pacheco, Maria Emilia
Synchronous Fluorescence Spectrometry
Inner Filter Effect
Bovine Serum Albumin
Imazethapyr
Chlorimuron-Ethyl
https://purl.org/becyt/ford/1.4
https://purl.org/becyt/ford/1
title_short Synchronous fluorescence spectrometry: Conformational investigation or inner filter effect?
title_full Synchronous fluorescence spectrometry: Conformational investigation or inner filter effect?
title_fullStr Synchronous fluorescence spectrometry: Conformational investigation or inner filter effect?
title_full_unstemmed Synchronous fluorescence spectrometry: Conformational investigation or inner filter effect?
title_sort Synchronous fluorescence spectrometry: Conformational investigation or inner filter effect?
dc.creator.none.fl_str_mv Pacheco, Maria Emilia
Bruzzone, Liliana
author Pacheco, Maria Emilia
author_facet Pacheco, Maria Emilia
Bruzzone, Liliana
author_role author
author2 Bruzzone, Liliana
author2_role author
dc.subject.none.fl_str_mv Synchronous Fluorescence Spectrometry
Inner Filter Effect
Bovine Serum Albumin
Imazethapyr
Chlorimuron-Ethyl
https://purl.org/becyt/ford/1.4
https://purl.org/becyt/ford/1
topic Synchronous Fluorescence Spectrometry
Inner Filter Effect
Bovine Serum Albumin
Imazethapyr
Chlorimuron-Ethyl
https://purl.org/becyt/ford/1.4
https://purl.org/becyt/ford/1
description Synchronous fluorescence spectrometry has become a common tool to investigate conformational changes in proteins due to the presence of other molecules (quenchers). The same pattern is always followed, leaving aside the possibility of interfering effects that make conclusions incorrect. In this paper, we discuss the main cause of a wrong understanding of synchronous fluorescence spectrometry in a large number of papers published in many different journals. Based on the principles of synchronous fluorescence spectrometry, we perform a simple correction method of synchronous spectra taking into account inner filter effects. This criticism tries to demonstrate that the interpretation of synchronous fluorescence spectrometry is not as easy as it seems.
publishDate 2013
dc.date.none.fl_str_mv 2013-01
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/22862
Pacheco, Maria Emilia; Bruzzone, Liliana; Synchronous fluorescence spectrometry: Conformational investigation or inner filter effect?; Elsevier Science; Journal of Luminescence; 137; 1-2013; 138-142
0022-2313
CONICET Digital
CONICET
url http://hdl.handle.net/11336/22862
identifier_str_mv Pacheco, Maria Emilia; Bruzzone, Liliana; Synchronous fluorescence spectrometry: Conformational investigation or inner filter effect?; Elsevier Science; Journal of Luminescence; 137; 1-2013; 138-142
0022-2313
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/doi/10.1016/j.jlumin.2012.12.056
info:eu-repo/semantics/altIdentifier/url/http://www.sciencedirect.com/science/article/pii/S0022231313000021
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-nd/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-nd/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
dc.publisher.none.fl_str_mv Elsevier Science
publisher.none.fl_str_mv Elsevier Science
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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score 15.811543

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