Impact of sub-lethal 2(3)-tert-butyl-4 hydroxyanisole (BHA) microcapsules on behavior of Aspergillus flavus in culture medium and peanut seeds

The aim of this work was the evaluation of physiological behavior of Aspergillus flavavus affected by the application of sub-lethal doses of microencapsulated 2(3)-tert-butyl-4 hydroxyanisole (BHA). For this , peanut meal extract agar (PMEA) and peanut kernels with modified water activity (aw) (0.96...

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Detalles Bibliográficos
Autores: García, Daiana, Girardi, Natalia Soledad, Nesci, Andrea Verónica, Passone, Maria Alejandra, Etcheverry, Miriam Graciela
Tipo de recurso: artículo
Estado:Versión publicada
Fecha de publicación:2016
País:Argentina
Institución:Consejo Nacional de Investigaciones Científicas y Técnicas
Repositorio:CONICET Digital (CONICET)
Idioma:inglés
OAI Identifier:oai:ri.conicet.gov.ar:11336/179624
Acceso en línea:http://hdl.handle.net/11336/179624
Access Level:acceso abierto
Palabra clave:PEANUT
ASPERGILLUS FLAVUS
AFLATOXIN B1
MICROCAPSULES
BHA
https://purl.org/becyt/ford/1.6
https://purl.org/becyt/ford/1
Descripción
Sumario:The aim of this work was the evaluation of physiological behavior of Aspergillus flavavus affected by the application of sub-lethal doses of microencapsulated 2(3)-tert-butyl-4 hydroxyanisole (BHA). For this , peanut meal extract agar (PMEA) and peanut kernels with modified water activity (aw) (0.96 and 0.99) and a sub-lethal dose (0.6 mM) of an BHA formulation was used. Fungl physiological aspects as growth rate, tme to growt and AFB1 evolution were modified by the formulation, especially on PMEA at both aw. In general, conidial and vesicle size of the mold only were affected by growth substrate, being higher on sterile seed than on PMEA regardless of aw condition . However, positive correlation (p<0.05) observed in control betweenradius and biomass responses was altedred by the application of the formulation, mainly under the lowest aw in both substrate evaluated. Finally, presence of encapsulated antioxidant showed significant change in Pearson coefficients respect to the controls for all studiend parameters.As conclusin, sub-lethaldoses formulation lead in reduction on growt and toxin accumulation, but conidial and vesicle size were not affected. Results of this work indicate the need to consider both fungal primary and secondary metabolism to determine the effect of food grade antioxidants formulation.