A conventional PCR technique to detect Septoria tritici in wheat seeds
A PCR assay was developed for detection of wheat seed naturally contaminated with <em>Septoria tritici. S. tritici </em>specific primers were derived from strict alignment of ITS and a-tubulin sequences of the pathogen. The specificity of four sets of synthesised oligonucleotide pairs (A...
| Autores: | , , , , |
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| Tipo de recurso: | artículo |
| Estado: | Versión enviada para evaluación y publicación |
| Fecha de publicación: | 2009 |
| País: | Argentina |
| Institución: | Comisión de Investigaciones Científicas de la Provincia de Buenos Aires |
| Repositorio: | CIC Digital (CICBA) |
| Idioma: | inglés |
| OAI Identifier: | oai:digital.cic.gba.gob.ar:11746/6993 |
| Acceso en línea: | https://digital.cic.gba.gob.ar/handle/11746/6993 |
| Access Level: | acceso abierto |
| Palabra clave: | Agronomía, reproducción y protección de plantas Mycosphaerella graminicola seedborne pathogen Septoria leaf blotch wheat |
| Sumario: | A PCR assay was developed for detection of wheat seed naturally contaminated with <em>Septoria tritici. S. tritici </em>specific primers were derived from strict alignment of ITS and a-tubulin sequences of the pathogen. The specificity of four sets of synthesised oligonucleotide pairs (A, B, C and D) were tested using isolates from <em>S. tritici</em>, other selected fungi and wheat seeds. A single DNA fragment was amplified from <em>S. tritici i</em>solates with all primer pairs, whereas no product was generated from otherDNAsources.<em> S. tritici </em>was also detected in wheat seed lots collected from plants with variable pycnidial coverage on the upper two leaves. PCR detection of as little as 0.5 pg of <em>S. tritici</em> genomic DNAwas possible. This is the first report on the detection of<em> S. tritici </em>DNA in naturally infested wheat seeds. This PCR based assay is simple, rapid, specific, sensitive and suitable for routine detection of the wheat pathogen in infested wheat seeds. |
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