Supramolecular analytical chemistry: Spectrofluorimetric determination of 6-hydroxymelatonin with hydroxypropyl-β-cyclodextrin
The effect of the addition of native (α, β and γ) and derivative (methyl-β and hydroxypropyl-β) cyclodextrins (CD) on the fluorescence of 6-hydroxymelatonin (6HM) in water solutions was studied. The fluorescence of the substrate in the presence of hydroxypropyl-β-CD (HPCD) showed the maximum enhance...
| Authors: | , , |
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| Format: | article |
| Status: | Published version |
| Publication Date: | 2011 |
| Country: | Argentina |
| Institution: | Consejo Nacional de Investigaciones Científicas y Técnicas |
| Repository: | CONICET Digital (CONICET) |
| Language: | English |
| OAI Identifier: | oai:ri.conicet.gov.ar:11336/61852 |
| Online Access: | http://hdl.handle.net/11336/61852 |
| Access Level: | Open access |
| Keyword: | 6-HYDROXYMELATONIN CYCLODEXTRIN DETERMINATION SPECTROFLUORIMETRY SUPRAMOLECULAR INTERACTION https://purl.org/becyt/ford/1.4 https://purl.org/becyt/ford/1 |
| Summary: | The effect of the addition of native (α, β and γ) and derivative (methyl-β and hydroxypropyl-β) cyclodextrins (CD) on the fluorescence of 6-hydroxymelatonin (6HM) in water solutions was studied. The fluorescence of the substrate in the presence of hydroxypropyl-β-CD (HPCD) showed the maximum enhancement and the association constant (KA, mol-1 L) was determined (60±2) and interpreted. The fluorescence quantum yield ratio between the complex and free substrate (φ6HMCD/φ6HM) = 6.0±0.2 provides analytical advantages. On the basis of this supramolecular interaction, an alternative and sensitive spectrofluorimetric method for the determination of 6HM was developed with a detection limit of 0.71 ng mL-1. The validation of the method was performed in urine samples with very good recoveries 95-109 %. |
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