N-glycan trimming by glucosidase II is essential for Arabidopsis development

Glucosidase II, one of the early N-glycan processing enzymes and a major player in the glycoprotein folding quality control, has been described as a soluble heterodimer composed of alpha and beta subunits. Here we present the first characterization of a plant glucosidase II alpha subunit at the mole...

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Autores: Soussilane, Pravina, D'Alessio, Cecilia, Paccalet, Thomas, Fitchette, Anne-Catherine, Parodi, Armando José A., Williamson, Richard, Plasson, Carole, Faye, Loic, Gomord, Veronique
Tipo de recurso: artículo
Estado:Versión publicada
Fecha de publicación:2008
País:Argentina
Institución:Consejo Nacional de Investigaciones Científicas y Técnicas
Repositorio:CONICET Digital (CONICET)
Idioma:inglés
OAI Identifier:oai:ri.conicet.gov.ar:11336/25550
Acceso en línea:http://hdl.handle.net/11336/25550
Access Level:acceso abierto
Palabra clave:Plant
N-Glycosylation
Glucosidase Ii
Glucose Trimming
https://purl.org/becyt/ford/1.6
https://purl.org/becyt/ford/1
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repository_id_str
spelling N-glycan trimming by glucosidase II is essential for Arabidopsis developmentSoussilane, PravinaD'Alessio, CeciliaPaccalet, ThomasFitchette, Anne-CatherineParodi, Armando José A.Williamson, RichardPlasson, CaroleFaye, LoicGomord, VeroniquePlantN-GlycosylationGlucosidase IiGlucose Trimminghttps://purl.org/becyt/ford/1.6https://purl.org/becyt/ford/1Glucosidase II, one of the early N-glycan processing enzymes and a major player in the glycoprotein folding quality control, has been described as a soluble heterodimer composed of alpha and beta subunits. Here we present the first characterization of a plant glucosidase II alpha subunit at the molecular level. Expression of the Arabidopsis alpha subunit restored N-glycan maturation capacity in Schizosaccharomyces pombe alpha- or alphabeta-deficient mutants, but with a lower efficiency in the last case. Inactivation of the alpha subunit in a temperature sensitive Arabidopsis mutant blocked N-glycan processing after a first trimming by glucosidase I and strongly affected seedling development.Fil: Soussilane, Pravina. Centre National de la Recherche Scientifique; FranciaFil: D'Alessio, Cecilia. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Parque Centenario. Instituto de Investigaciones Bioquímicas de Buenos Aires. Fundación Instituto Leloir. Instituto de Investigaciones Bioquímicas de Buenos Aires; ArgentinaFil: Paccalet, Thomas. Centre National de la Recherche Scientifique; FranciaFil: Fitchette, Anne-Catherine. Centre National de la Recherche Scientifique; FranciaFil: Parodi, Armando José A.. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Parque Centenario. Instituto de Investigaciones Bioquímicas de Buenos Aires. Fundación Instituto Leloir. Instituto de Investigaciones Bioquímicas de Buenos Aires; ArgentinaFil: Williamson, Richard. The Australian National University; AustraliaFil: Plasson, Carole. Centre National de la Recherche Scientifique; FranciaFil: Faye, Loic. Centre National de la Recherche Scientifique; FranciaFil: Gomord, Veronique. Centre National de la Recherche Scientifique; FranciaSpringer2008-10info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/25550Soussilane, Pravina; D'Alessio, Cecilia; Paccalet, Thomas; Fitchette, Anne-Catherine; Parodi, Armando José A.; et al.; N-glycan trimming by glucosidase II is essential for Arabidopsis development; Springer; Glycoconjugate Journal; 26; 5; 10-2008; 597-6070282-00801573-4986CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/url/https://link.springer.com/article/10.1007%2Fs10719-008-9201-1info:eu-repo/semantics/altIdentifier/doi/10.1007/s10719-008-9201-1info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2024-05-08T13:37:22Zoai:ri.conicet.gov.ar:11336/25550instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982024-05-08 13:37:23.035CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv N-glycan trimming by glucosidase II is essential for Arabidopsis development
title N-glycan trimming by glucosidase II is essential for Arabidopsis development
spellingShingle N-glycan trimming by glucosidase II is essential for Arabidopsis development
Soussilane, Pravina
Plant
N-Glycosylation
Glucosidase Ii
Glucose Trimming
https://purl.org/becyt/ford/1.6
https://purl.org/becyt/ford/1
title_short N-glycan trimming by glucosidase II is essential for Arabidopsis development
title_full N-glycan trimming by glucosidase II is essential for Arabidopsis development
title_fullStr N-glycan trimming by glucosidase II is essential for Arabidopsis development
title_full_unstemmed N-glycan trimming by glucosidase II is essential for Arabidopsis development
title_sort N-glycan trimming by glucosidase II is essential for Arabidopsis development
dc.creator.none.fl_str_mv Soussilane, Pravina
D'Alessio, Cecilia
Paccalet, Thomas
Fitchette, Anne-Catherine
Parodi, Armando José A.
Williamson, Richard
Plasson, Carole
Faye, Loic
Gomord, Veronique
author Soussilane, Pravina
author_facet Soussilane, Pravina
D'Alessio, Cecilia
Paccalet, Thomas
Fitchette, Anne-Catherine
Parodi, Armando José A.
Williamson, Richard
Plasson, Carole
Faye, Loic
Gomord, Veronique
author_role author
author2 D'Alessio, Cecilia
Paccalet, Thomas
Fitchette, Anne-Catherine
Parodi, Armando José A.
Williamson, Richard
Plasson, Carole
Faye, Loic
Gomord, Veronique
author2_role author
author
author
author
author
author
author
author
dc.subject.none.fl_str_mv Plant
N-Glycosylation
Glucosidase Ii
Glucose Trimming
https://purl.org/becyt/ford/1.6
https://purl.org/becyt/ford/1
topic Plant
N-Glycosylation
Glucosidase Ii
Glucose Trimming
https://purl.org/becyt/ford/1.6
https://purl.org/becyt/ford/1
description Glucosidase II, one of the early N-glycan processing enzymes and a major player in the glycoprotein folding quality control, has been described as a soluble heterodimer composed of alpha and beta subunits. Here we present the first characterization of a plant glucosidase II alpha subunit at the molecular level. Expression of the Arabidopsis alpha subunit restored N-glycan maturation capacity in Schizosaccharomyces pombe alpha- or alphabeta-deficient mutants, but with a lower efficiency in the last case. Inactivation of the alpha subunit in a temperature sensitive Arabidopsis mutant blocked N-glycan processing after a first trimming by glucosidase I and strongly affected seedling development.
publishDate 2008
dc.date.none.fl_str_mv 2008-10
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/25550
Soussilane, Pravina; D'Alessio, Cecilia; Paccalet, Thomas; Fitchette, Anne-Catherine; Parodi, Armando José A.; et al.; N-glycan trimming by glucosidase II is essential for Arabidopsis development; Springer; Glycoconjugate Journal; 26; 5; 10-2008; 597-607
0282-0080
1573-4986
CONICET Digital
CONICET
url http://hdl.handle.net/11336/25550
identifier_str_mv Soussilane, Pravina; D'Alessio, Cecilia; Paccalet, Thomas; Fitchette, Anne-Catherine; Parodi, Armando José A.; et al.; N-glycan trimming by glucosidase II is essential for Arabidopsis development; Springer; Glycoconjugate Journal; 26; 5; 10-2008; 597-607
0282-0080
1573-4986
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/url/https://link.springer.com/article/10.1007%2Fs10719-008-9201-1
info:eu-repo/semantics/altIdentifier/doi/10.1007/s10719-008-9201-1
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
application/pdf
dc.publisher.none.fl_str_mv Springer
publisher.none.fl_str_mv Springer
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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score 15,812429

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