Sodium tetrathionate effect on papain purification from different <i>Carica papaya</i> latex crude extracts

Papain from latex of Carica papaya was purified up to matrix-assisted laser desorption= ionization (MALDI) time-of-flight (TOF) mass spectrometry homogeneity by salt precipitation from two different crude extract sources: a refined preparation obtained in our laboratory and a commercial one. Sodium...

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Autores: Llerena Suster, Carlos Rafael, Priolo de Lufrano, Nora Silvia, Morcelle del Valle, Susana Raquel
Tipo de recurso: artículo
Estado:Versión publicada
Fecha de publicación:2011
País:Argentina
Institución:Universidad Nacional de La Plata
Repositorio:SEDICI (UNLP)
Idioma:inglés
OAI Identifier:oai:sedici.unlp.edu.ar:10915/92383
Acceso en línea:http://sedici.unlp.edu.ar/handle/10915/92383
Access Level:acceso abierto
Palabra clave:Biología
Papain
Plant cysteine peptidases
Protein purification
Sodium tetrathionate
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spelling Sodium tetrathionate effect on papain purification from different <i>Carica papaya</i> latex crude extractsLlerena Suster, Carlos RafaelPriolo de Lufrano, Nora SilviaMorcelle del Valle, Susana RaquelBiologíaPapainPlant cysteine peptidasesProtein purificationSodium tetrathionatePapain from latex of Carica papaya was purified up to matrix-assisted laser desorption= ionization (MALDI) time-of-flight (TOF) mass spectrometry homogeneity by salt precipitation from two different crude extract sources: a refined preparation obtained in our laboratory and a commercial one. Sodium tetrathionate was tested in the purification process to preserve the enzymatic activity of the peptidase. Purification was checked by sodium dodecyl sulfate (SDS) polyacrylamide gel electrophoresis (PAGE) and cation exchange chromatography, using commercial pure papain as standard for a rapid comparison. The best purification yields (3.4%) were obtained in presence of 30 mM sodium tetrathionate for the crude extract prepared in our laboratory. The described purification method proved to be robust and reliable to obtain pure papain on a preparative scale.Centro de Investigación de Proteínas Vegetales2011info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionArticulohttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdf107-121http://sedici.unlp.edu.ar/handle/10915/92383enginfo:eu-repo/semantics/altIdentifier/issn/1532-2297info:eu-repo/semantics/altIdentifier/doi/10.1080/10826068.2011.544230info:eu-repo/semantics/openAccesshttp://creativecommons.org/licenses/by-nc-sa/4.0/Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International (CC BY-NC-SA 4.0)reponame:SEDICI (UNLP)instname:Universidad Nacional de La Platainstacron:UNLP2024-05-08T12:57:09Zoai:sedici.unlp.edu.ar:10915/92383Institucionalhttp://sedici.unlp.edu.ar/Universidad públicaNo correspondehttp://sedici.unlp.edu.ar/oai/snrdalira@sedici.unlp.edu.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:13292024-05-08 12:57:09.823SEDICI (UNLP) - Universidad Nacional de La Platafalse
dc.title.none.fl_str_mv Sodium tetrathionate effect on papain purification from different <i>Carica papaya</i> latex crude extracts
title Sodium tetrathionate effect on papain purification from different <i>Carica papaya</i> latex crude extracts
spellingShingle Sodium tetrathionate effect on papain purification from different <i>Carica papaya</i> latex crude extracts
Llerena Suster, Carlos Rafael
Biología
Papain
Plant cysteine peptidases
Protein purification
Sodium tetrathionate
title_short Sodium tetrathionate effect on papain purification from different <i>Carica papaya</i> latex crude extracts
title_full Sodium tetrathionate effect on papain purification from different <i>Carica papaya</i> latex crude extracts
title_fullStr Sodium tetrathionate effect on papain purification from different <i>Carica papaya</i> latex crude extracts
title_full_unstemmed Sodium tetrathionate effect on papain purification from different <i>Carica papaya</i> latex crude extracts
title_sort Sodium tetrathionate effect on papain purification from different <i>Carica papaya</i> latex crude extracts
dc.creator.none.fl_str_mv Llerena Suster, Carlos Rafael
Priolo de Lufrano, Nora Silvia
Morcelle del Valle, Susana Raquel
author Llerena Suster, Carlos Rafael
author_facet Llerena Suster, Carlos Rafael
Priolo de Lufrano, Nora Silvia
Morcelle del Valle, Susana Raquel
author_role author
author2 Priolo de Lufrano, Nora Silvia
Morcelle del Valle, Susana Raquel
author2_role author
author
dc.subject.none.fl_str_mv Biología
Papain
Plant cysteine peptidases
Protein purification
Sodium tetrathionate
topic Biología
Papain
Plant cysteine peptidases
Protein purification
Sodium tetrathionate
description Papain from latex of Carica papaya was purified up to matrix-assisted laser desorption= ionization (MALDI) time-of-flight (TOF) mass spectrometry homogeneity by salt precipitation from two different crude extract sources: a refined preparation obtained in our laboratory and a commercial one. Sodium tetrathionate was tested in the purification process to preserve the enzymatic activity of the peptidase. Purification was checked by sodium dodecyl sulfate (SDS) polyacrylamide gel electrophoresis (PAGE) and cation exchange chromatography, using commercial pure papain as standard for a rapid comparison. The best purification yields (3.4%) were obtained in presence of 30 mM sodium tetrathionate for the crude extract prepared in our laboratory. The described purification method proved to be robust and reliable to obtain pure papain on a preparative scale.
publishDate 2011
dc.date.none.fl_str_mv 2011
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
Articulo
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://sedici.unlp.edu.ar/handle/10915/92383
url http://sedici.unlp.edu.ar/handle/10915/92383
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/issn/1532-2297
info:eu-repo/semantics/altIdentifier/doi/10.1080/10826068.2011.544230
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
http://creativecommons.org/licenses/by-nc-sa/4.0/
Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International (CC BY-NC-SA 4.0)
eu_rights_str_mv openAccess
rights_invalid_str_mv http://creativecommons.org/licenses/by-nc-sa/4.0/
Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International (CC BY-NC-SA 4.0)
dc.format.none.fl_str_mv application/pdf
107-121
dc.source.none.fl_str_mv reponame:SEDICI (UNLP)
instname:Universidad Nacional de La Plata
instacron:UNLP
instname_str Universidad Nacional de La Plata
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collection SEDICI (UNLP)
repository.name.fl_str_mv SEDICI (UNLP) - Universidad Nacional de La Plata
repository.mail.fl_str_mv alira@sedici.unlp.edu.ar
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