A simply method for the addition of rotenone in Arabidopsis thaliana leaves
A simple and reproducible method for the treatment of Arabidopsis thalianaleaves with rotenone is presented. Rosette leaves were incubated with rotenone andTriton X-100 for at least 15 h. Treated leaves showed increased expression of COX19 and BCS1a, two genes known to be induced in Arabidopsis cell...
| Autores: | , , , |
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| Tipo de recurso: | artículo |
| Estado: | Versión publicada |
| Fecha de publicación: | 2015 |
| País: | Argentina |
| Institución: | Consejo Nacional de Investigaciones Científicas y Técnicas |
| Repositorio: | CONICET Digital (CONICET) |
| Idioma: | inglés |
| OAI Identifier: | oai:ri.conicet.gov.ar:11336/7826 |
| Acceso en línea: | http://hdl.handle.net/11336/7826 |
| Access Level: | acceso abierto |
| Palabra clave: | Rotenone Arabidopsis Thaliana Mitochondria Mitochondrial Respiration Complex I https://purl.org/becyt/ford/1.6 https://purl.org/becyt/ford/1 |
| Sumario: | A simple and reproducible method for the treatment of Arabidopsis thalianaleaves with rotenone is presented. Rosette leaves were incubated with rotenone andTriton X-100 for at least 15 h. Treated leaves showed increased expression of COX19 and BCS1a, two genes known to be induced in Arabidopsis cell cultures afterrotenone treatment. Moreover, rotenone/Triton X-100 incubated leaves presented aninhibition of oxygen uptake. The simplicity of the procedure shows this methodology useful for studying the effect of the addition of rotenone to a photosynthetic tissue in situ. |
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