FISH-mapping of the 5S rDNA locus in chili peppers (Capsicum-Solanaceae)

We present here the physical mapping of the 5S rDNA locus in six wild and five cultivated taxa of Capsicum by means of a genus-specific FISH probe. In all taxa, a single 5S locus per haploid genome that persistentlymapped onto the short arm of a unique metacentric chromosome pair at intercalar posit...

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Detalles Bibliográficos
Autores: Aguilera, Patricia Mabel, Debat, Humberto Julio, Scaldaferro, Marisel Analía, Marti, Dardo Andrea, Grabiele, Mauro
Tipo de recurso: artículo
Estado:Versión publicada
Fecha de publicación:2016
País:Argentina
Institución:Consejo Nacional de Investigaciones Científicas y Técnicas
Repositorio:CONICET Digital (CONICET)
Idioma:inglés
OAI Identifier:oai:ri.conicet.gov.ar:11336/23255
Acceso en línea:http://hdl.handle.net/11336/23255
Access Level:acceso abierto
Palabra clave:WILD AND CULTIVATED CAPSICUM
5S rRNA GENE
CAPSICUM-DERIVE PROVE
FISH-MAPPING
https://purl.org/becyt/ford/1.6
https://purl.org/becyt/ford/1
Descripción
Sumario:We present here the physical mapping of the 5S rDNA locus in six wild and five cultivated taxa of Capsicum by means of a genus-specific FISH probe. In all taxa, a single 5S locus per haploid genome that persistentlymapped onto the short arm of a unique metacentric chromosome pair at intercalar position, was found. 5S FISH signals of almost the same size and brightness intensity were observed in all the analyzed taxa. Thisis the first cytological characterization of the 5S in wild taxa of Capsicum by using a genus-derived probe, and the most exhaustive and comprehensive in the chili peppers up to now. The information providedhere will aid the cytomolecular characterization of pepper germplasm to evaluate variability and can be instrumental to integrate physical, genetic and genomic maps already generated in the genus.