Chemical hypoxia induces apoptosis of human pluripotent stem cells by a NOXA-mediated HIF-1α and HIF-2α independent mechanism

Human embryonic and induced pluripotent stem cells (hESCs and hiPSCs) are self-renewing human pluripotent stem cells (hPSCs) that can differentiate to a wide range of specialized cells. Notably, hPSCs enhance their undifferentiated state and self-renewal properties in hypoxia (5% O2). Although thoro...

Descripción completa

Detalles Bibliográficos
Autores: Isaja, Luciana, Mucci, Sofía, Vera, Jonathan, Rodríguez Varela, Maria Soledad, Marazita, Mariela Claudia, Morris Hanon, Olivia, Videla Richardson, Guillermo Agustín, Sevlever, Gustavo Emilio, Scassa, Maria Elida, Romorini, Leonardo
Tipo de recurso: artículo
Estado:Versión publicada
Fecha de publicación:2020
País:Argentina
Institución:Consejo Nacional de Investigaciones Científicas y Técnicas
Repositorio:CONICET Digital (CONICET)
Idioma:inglés
OAI Identifier:oai:ri.conicet.gov.ar:11336/182508
Acceso en línea:http://hdl.handle.net/11336/182508
Access Level:acceso abierto
Palabra clave:HIF-1α
HIF-2α
Hypoxia
Human pluripotent stem cells
https://purl.org/becyt/ford/1.6
https://purl.org/becyt/ford/1
id AR_65dcf40a2e6fa1a02e8faf2cef696e78
oai_identifier_str oai:ri.conicet.gov.ar:11336/182508
network_acronym_str AR
network_name_str Argentina
repository_id_str
dc.title.none.fl_str_mv Chemical hypoxia induces apoptosis of human pluripotent stem cells by a NOXA-mediated HIF-1α and HIF-2α independent mechanism
title Chemical hypoxia induces apoptosis of human pluripotent stem cells by a NOXA-mediated HIF-1α and HIF-2α independent mechanism
spellingShingle Chemical hypoxia induces apoptosis of human pluripotent stem cells by a NOXA-mediated HIF-1α and HIF-2α independent mechanism
Isaja, Luciana
HIF-1α
HIF-2α
Hypoxia
Human pluripotent stem cells
https://purl.org/becyt/ford/1.6
https://purl.org/becyt/ford/1
title_short Chemical hypoxia induces apoptosis of human pluripotent stem cells by a NOXA-mediated HIF-1α and HIF-2α independent mechanism
title_full Chemical hypoxia induces apoptosis of human pluripotent stem cells by a NOXA-mediated HIF-1α and HIF-2α independent mechanism
title_fullStr Chemical hypoxia induces apoptosis of human pluripotent stem cells by a NOXA-mediated HIF-1α and HIF-2α independent mechanism
title_full_unstemmed Chemical hypoxia induces apoptosis of human pluripotent stem cells by a NOXA-mediated HIF-1α and HIF-2α independent mechanism
title_sort Chemical hypoxia induces apoptosis of human pluripotent stem cells by a NOXA-mediated HIF-1α and HIF-2α independent mechanism
dc.creator.none.fl_str_mv Isaja, Luciana
Mucci, Sofía
Vera, Jonathan
Rodríguez Varela, Maria Soledad
Marazita, Mariela Claudia
Morris Hanon, Olivia
Videla Richardson, Guillermo Agustín
Sevlever, Gustavo Emilio
Scassa, Maria Elida
Romorini, Leonardo
author Isaja, Luciana
author_facet Isaja, Luciana
Mucci, Sofía
Vera, Jonathan
Rodríguez Varela, Maria Soledad
Marazita, Mariela Claudia
Morris Hanon, Olivia
Videla Richardson, Guillermo Agustín
Sevlever, Gustavo Emilio
Scassa, Maria Elida
Romorini, Leonardo
author_role author
author2 Mucci, Sofía
Vera, Jonathan
Rodríguez Varela, Maria Soledad
Marazita, Mariela Claudia
Morris Hanon, Olivia
Videla Richardson, Guillermo Agustín
Sevlever, Gustavo Emilio
Scassa, Maria Elida
Romorini, Leonardo
author2_role author
author
author
author
author
author
author
author
author
dc.subject.none.fl_str_mv HIF-1α
HIF-2α
Hypoxia
Human pluripotent stem cells
https://purl.org/becyt/ford/1.6
https://purl.org/becyt/ford/1
topic HIF-1α
HIF-2α
Hypoxia
Human pluripotent stem cells
https://purl.org/becyt/ford/1.6
https://purl.org/becyt/ford/1
description Human embryonic and induced pluripotent stem cells (hESCs and hiPSCs) are self-renewing human pluripotent stem cells (hPSCs) that can differentiate to a wide range of specialized cells. Notably, hPSCs enhance their undifferentiated state and self-renewal properties in hypoxia (5% O2). Although thoroughly analyzed, hypoxia implication in hPSCs death is not fully determined. In order to evaluate the effect of chemically mimicked hypoxia on hPSCs cell survival, we analyzed changes in cell viability and several aspects of apoptosis triggered by CoCl2 and dimethyloxalylglycine (DMOG). Mitochondrial function assays revealed a decrease in cell viability at 24 h post-treatments. Moreover, we detected chromatin condensation, DNA fragmentation and CASPASE-9 and 3 cleavages. In this context, we observed that P53, BNIP-3, and NOXA protein expression levels were significantly up-regulated at different time points upon chemical hypoxia induction. However, only siRNA-mediated downregulation of NOXA but not HIF-1α, HIF-2α, BNIP-3, and P53 did significantly affect the extent of cell death triggered by CoCl2 and DMOG in hPSCs. In conclusion, chemically mimicked hypoxia induces hPSCs cell death by a NOXA-mediated HIF-1α and HIF-2α independent mechanism.
publishDate 2020
dc.date.none.fl_str_mv 2020-12
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/182508
Isaja, Luciana; Mucci, Sofía; Vera, Jonathan; Rodríguez Varela, Maria Soledad; Marazita, Mariela Claudia; et al.; Chemical hypoxia induces apoptosis of human pluripotent stem cells by a NOXA-mediated HIF-1α and HIF-2α independent mechanism; Nature Publishing Group; Scientific Reports; 10; 1; 12-2020; 1-15
2045-2322
CONICET Digital
CONICET
url http://hdl.handle.net/11336/182508
identifier_str_mv Isaja, Luciana; Mucci, Sofía; Vera, Jonathan; Rodríguez Varela, Maria Soledad; Marazita, Mariela Claudia; et al.; Chemical hypoxia induces apoptosis of human pluripotent stem cells by a NOXA-mediated HIF-1α and HIF-2α independent mechanism; Nature Publishing Group; Scientific Reports; 10; 1; 12-2020; 1-15
2045-2322
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/doi/10.1038/s41598-020-77792-7
info:eu-repo/semantics/altIdentifier/url/https://www.nature.com/articles/s41598-020-77792-7
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
dc.publisher.none.fl_str_mv Nature Publishing Group
publisher.none.fl_str_mv Nature Publishing Group
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
_version_ 1799196390882017280
spelling Chemical hypoxia induces apoptosis of human pluripotent stem cells by a NOXA-mediated HIF-1α and HIF-2α independent mechanismIsaja, LucianaMucci, SofíaVera, JonathanRodríguez Varela, Maria SoledadMarazita, Mariela ClaudiaMorris Hanon, OliviaVidela Richardson, Guillermo AgustínSevlever, Gustavo EmilioScassa, Maria ElidaRomorini, LeonardoHIF-1αHIF-2αHypoxiaHuman pluripotent stem cellshttps://purl.org/becyt/ford/1.6https://purl.org/becyt/ford/1Human embryonic and induced pluripotent stem cells (hESCs and hiPSCs) are self-renewing human pluripotent stem cells (hPSCs) that can differentiate to a wide range of specialized cells. Notably, hPSCs enhance their undifferentiated state and self-renewal properties in hypoxia (5% O2). Although thoroughly analyzed, hypoxia implication in hPSCs death is not fully determined. In order to evaluate the effect of chemically mimicked hypoxia on hPSCs cell survival, we analyzed changes in cell viability and several aspects of apoptosis triggered by CoCl2 and dimethyloxalylglycine (DMOG). Mitochondrial function assays revealed a decrease in cell viability at 24 h post-treatments. Moreover, we detected chromatin condensation, DNA fragmentation and CASPASE-9 and 3 cleavages. In this context, we observed that P53, BNIP-3, and NOXA protein expression levels were significantly up-regulated at different time points upon chemical hypoxia induction. However, only siRNA-mediated downregulation of NOXA but not HIF-1α, HIF-2α, BNIP-3, and P53 did significantly affect the extent of cell death triggered by CoCl2 and DMOG in hPSCs. In conclusion, chemically mimicked hypoxia induces hPSCs cell death by a NOXA-mediated HIF-1α and HIF-2α independent mechanism.Fil: Isaja, Luciana. Fundación para la Lucha contra las Enfermedades Neurológicas de la Infancia. Instituto de Neurociencias - Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Ciudad Universitaria. Instituto de Neurociencias; Argentina. Fundación para la Lucha contra las Enfermedades Neurológicas de la Infancia; ArgentinaFil: Mucci, Sofía. Fundación para la Lucha contra las Enfermedades Neurológicas de la Infancia; ArgentinaFil: Vera, Jonathan. Fundación para la Lucha contra las Enfermedades Neurológicas de la Infancia; ArgentinaFil: Rodríguez Varela, Maria Soledad. Fundación para la Lucha contra las Enfermedades Neurológicas de la Infancia. Instituto de Neurociencias - Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Ciudad Universitaria. Instituto de Neurociencias; Argentina. Fundación para la Lucha contra las Enfermedades Neurológicas de la Infancia; ArgentinaFil: Marazita, Mariela Claudia. Fundación para la Lucha contra las Enfermedades Neurológicas de la Infancia. Instituto de Neurociencias - Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Ciudad Universitaria. Instituto de Neurociencias; Argentina. Fundación para la Lucha contra las Enfermedades Neurológicas de la Infancia; ArgentinaFil: Morris Hanon, Olivia. Fundación para la Lucha contra las Enfermedades Neurológicas de la Infancia. Instituto de Neurociencias - Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Ciudad Universitaria. Instituto de Neurociencias; Argentina. Fundación para la Lucha contra las Enfermedades Neurológicas de la Infancia; ArgentinaFil: Videla Richardson, Guillermo Agustín. Fundación para la Lucha contra las Enfermedades Neurológicas de la Infancia; ArgentinaFil: Sevlever, Gustavo Emilio. Fundación para la Lucha contra las Enfermedades Neurológicas de la Infancia; ArgentinaFil: Scassa, Maria Elida. Fundación para la Lucha contra las Enfermedades Neurológicas de la Infancia; ArgentinaFil: Romorini, Leonardo. Fundación para la Lucha contra las Enfermedades Neurológicas de la Infancia. Instituto de Neurociencias - Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Ciudad Universitaria. Instituto de Neurociencias; Argentina. Fundación para la Lucha contra las Enfermedades Neurológicas de la Infancia; ArgentinaNature Publishing Group2020-12info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/182508Isaja, Luciana; Mucci, Sofía; Vera, Jonathan; Rodríguez Varela, Maria Soledad; Marazita, Mariela Claudia; et al.; Chemical hypoxia induces apoptosis of human pluripotent stem cells by a NOXA-mediated HIF-1α and HIF-2α independent mechanism; Nature Publishing Group; Scientific Reports; 10; 1; 12-2020; 1-152045-2322CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/doi/10.1038/s41598-020-77792-7info:eu-repo/semantics/altIdentifier/url/https://www.nature.com/articles/s41598-020-77792-7info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2024-05-08T14:25:52Zoai:ri.conicet.gov.ar:11336/182508instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982024-05-08 14:25:52.839CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
score 15,811543

Similares en LA Referencia