A derivative UV spectrophotometry method for the determination of levothyroxine sodium in tablets

A derivative UV (DUV) spectrophotometric method was developed for the determination of Levothyroxine Sodium (LT4) in tablets of different doses. Quantification was performed using the second derivative of the absorption spectrum at 253 nm (2D253) in methanol : water (50 : 50; v/v) (pH 11.2). The met...

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Detalles Bibliográficos
Autores: Gregorini, Anabella, Ruiz, María Esperanza, Volonté, Maria Guillermina
Tipo de recurso: artículo
Estado:Versión publicada
Fecha de publicación:2013
País:Argentina
Institución:Consejo Nacional de Investigaciones Científicas y Técnicas
Repositorio:CONICET Digital (CONICET)
Idioma:inglés
OAI Identifier:oai:ri.conicet.gov.ar:11336/4880
Acceso en línea:http://hdl.handle.net/11336/4880
Access Level:acceso abierto
Palabra clave:Levothyroxine Sodium
Derivative Uv Spectrophotometry
High Performance Liquid Chromatography
https://purl.org/becyt/ford/1.4
https://purl.org/becyt/ford/1
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spelling A derivative UV spectrophotometry method for the determination of levothyroxine sodium in tabletsGregorini, AnabellaRuiz, María EsperanzaVolonté, Maria GuillerminaLevothyroxine SodiumDerivative Uv SpectrophotometryHigh Performance Liquid Chromatographyhttps://purl.org/becyt/ford/1.4https://purl.org/becyt/ford/1A derivative UV (DUV) spectrophotometric method was developed for the determination of Levothyroxine Sodium (LT4) in tablets of different doses. Quantification was performed using the second derivative of the absorption spectrum at 253 nm (2D253) in methanol : water (50 : 50; v/v) (pH 11.2). The method was validated and compared with an HPLC procedure carried out using a RP18 column (125 x~ 4 mm, 5 microm) and methanol: phosphoric acid (0.1%) (70 : 30, v/v) (pH 3) as mobile phase. Flow rate was set at 1.5 mL/min, and detection was performed at 225 nm. The proposed DUV method was linear in the range 3.0 - 40.0 microg/mL with an appropriate precision and accuracy, and it was selective for the drug under study. On the other hand, results obtained by 2D253 analysis were similar to those obtained by HPLC, with no statistically significant differences between them. Therefore, it was concluded that the developed method is suitable for the determination of LT4 in tablets at the tested doses.Fil: Gregorini, Anabella. Universidad Nacional de la Plata. Facultad de Cs.exactas. Departamento de Cs.biologicas. Catedra de Control de Calidad de Medicamentos; ArgentinaFil: Ruiz, María Esperanza. Universidad Nacional de la Plata. Facultad de Cs.exactas. Departamento de Cs.biologicas. Catedra de Control de Calidad de Medicamentos; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico La Plata. Centro de Investigación y Desarrollo en Ciencias Aplicadas; ArgentinaFil: Volonté, Maria Guillermina. Universidad Nacional de la Plata. Facultad de Cs.exactas. Departamento de Cs.biologicas. Catedra de Control de Calidad de Medicamentos; ArgentinaSP MAIK Nauka/Interperiodica2013-06info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/4880Gregorini, Anabella; Ruiz, María Esperanza; Volonté, Maria Guillermina; A derivative UV spectrophotometry method for the determination of levothyroxine sodium in tablets; SP MAIK Nauka/Interperiodica; Journal Of Analytical Chemistry; 68; 6; 6-2013; 510-5151061-9348enginfo:eu-repo/semantics/altIdentifier/url/http://link.springer.com/article/10.1134/S1061934813060075info:eu-repo/semantics/altIdentifier/doi/10.1134/S1061934813060075info:eu-repo/semantics/altIdentifier/doi/info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2024-05-08T14:01:31Zoai:ri.conicet.gov.ar:11336/4880instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982024-05-08 14:01:31.988CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv A derivative UV spectrophotometry method for the determination of levothyroxine sodium in tablets
title A derivative UV spectrophotometry method for the determination of levothyroxine sodium in tablets
spellingShingle A derivative UV spectrophotometry method for the determination of levothyroxine sodium in tablets
Gregorini, Anabella
Levothyroxine Sodium
Derivative Uv Spectrophotometry
High Performance Liquid Chromatography
https://purl.org/becyt/ford/1.4
https://purl.org/becyt/ford/1
title_short A derivative UV spectrophotometry method for the determination of levothyroxine sodium in tablets
title_full A derivative UV spectrophotometry method for the determination of levothyroxine sodium in tablets
title_fullStr A derivative UV spectrophotometry method for the determination of levothyroxine sodium in tablets
title_full_unstemmed A derivative UV spectrophotometry method for the determination of levothyroxine sodium in tablets
title_sort A derivative UV spectrophotometry method for the determination of levothyroxine sodium in tablets
dc.creator.none.fl_str_mv Gregorini, Anabella
Ruiz, María Esperanza
Volonté, Maria Guillermina
author Gregorini, Anabella
author_facet Gregorini, Anabella
Ruiz, María Esperanza
Volonté, Maria Guillermina
author_role author
author2 Ruiz, María Esperanza
Volonté, Maria Guillermina
author2_role author
author
dc.subject.none.fl_str_mv Levothyroxine Sodium
Derivative Uv Spectrophotometry
High Performance Liquid Chromatography
https://purl.org/becyt/ford/1.4
https://purl.org/becyt/ford/1
topic Levothyroxine Sodium
Derivative Uv Spectrophotometry
High Performance Liquid Chromatography
https://purl.org/becyt/ford/1.4
https://purl.org/becyt/ford/1
description A derivative UV (DUV) spectrophotometric method was developed for the determination of Levothyroxine Sodium (LT4) in tablets of different doses. Quantification was performed using the second derivative of the absorption spectrum at 253 nm (2D253) in methanol : water (50 : 50; v/v) (pH 11.2). The method was validated and compared with an HPLC procedure carried out using a RP18 column (125 x~ 4 mm, 5 microm) and methanol: phosphoric acid (0.1%) (70 : 30, v/v) (pH 3) as mobile phase. Flow rate was set at 1.5 mL/min, and detection was performed at 225 nm. The proposed DUV method was linear in the range 3.0 - 40.0 microg/mL with an appropriate precision and accuracy, and it was selective for the drug under study. On the other hand, results obtained by 2D253 analysis were similar to those obtained by HPLC, with no statistically significant differences between them. Therefore, it was concluded that the developed method is suitable for the determination of LT4 in tablets at the tested doses.
publishDate 2013
dc.date.none.fl_str_mv 2013-06
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/4880
Gregorini, Anabella; Ruiz, María Esperanza; Volonté, Maria Guillermina; A derivative UV spectrophotometry method for the determination of levothyroxine sodium in tablets; SP MAIK Nauka/Interperiodica; Journal Of Analytical Chemistry; 68; 6; 6-2013; 510-515
1061-9348
url http://hdl.handle.net/11336/4880
identifier_str_mv Gregorini, Anabella; Ruiz, María Esperanza; Volonté, Maria Guillermina; A derivative UV spectrophotometry method for the determination of levothyroxine sodium in tablets; SP MAIK Nauka/Interperiodica; Journal Of Analytical Chemistry; 68; 6; 6-2013; 510-515
1061-9348
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/url/http://link.springer.com/article/10.1134/S1061934813060075
info:eu-repo/semantics/altIdentifier/doi/10.1134/S1061934813060075
info:eu-repo/semantics/altIdentifier/doi/
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
dc.publisher.none.fl_str_mv SP MAIK Nauka/Interperiodica
publisher.none.fl_str_mv SP MAIK Nauka/Interperiodica
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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score 15,812455

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