Ceramide-1-phosphate, a new mediator of development and survival in retina photoreceptors
<b>Purpose.</b> Simple sphingolipids control crucial cellular processes in several cell types. Previous work demonstrated that sphingolipids, such as ceramide, sphingosine, and sphingosine-1-phosphate, are key mediators in the regulation of survival, differentiation, and proliferation of...
| Autores: | , , , , |
|---|---|
| Tipo de recurso: | artículo |
| Estado: | Versión publicada |
| Fecha de publicación: | 2011 |
| País: | Argentina |
| Institución: | Universidad Nacional de La Plata |
| Repositorio: | SEDICI (UNLP) |
| Idioma: | inglés |
| OAI Identifier: | oai:sedici.unlp.edu.ar:10915/84041 |
| Acceso en línea: | http://sedici.unlp.edu.ar/handle/10915/84041 |
| Access Level: | acceso abierto |
| Palabra clave: | Biología Sphingolipids Ceramide-1-phosphate Retina Biología Celular |
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Ceramide-1-phosphate, a new mediator of development and survival in retina photoreceptorsMiranda, Gisela E.Abrahan, Carolina E.Agnolazza, Daniela L.Politi, Luis E.Rotstein, Nora P.BiologíaSphingolipidsCeramide-1-phosphateRetinaBiología Celular<b>Purpose.</b> Simple sphingolipids control crucial cellular processes in several cell types. Previous work demonstrated that sphingolipids, such as ceramide, sphingosine, and sphingosine-1-phosphate, are key mediators in the regulation of survival, differentiation, and proliferation of retina photoreceptors. Ceramide-1-phosphate (C1P) regulates growth and survival in several cell types; however, little is known concerning its functions in the retina. Whether C1P also participates in controlling photoreceptor development was also explored. <b>Methods.</b> Rat retina neuronal cultures were supplemented with 1 to 10 μM C1P. Proliferation was determined by evaluating 5-bromo-2-deoxyuridine (BrdU) uptake and the number of mitotic figures and differentiation by evaluating opsin and peripherin expression by immunocytochemistry and Western blot. Apoptosis was inhibited with the pan caspase inhibitor ZVADFMK and evaluated by TUNEL assay, propidium iodide/annexin V, and DAPI labeling. Preservation of mitochondrial membrane potential was evaluated. <b>Results.</b> C1P enhanced BrdU uptake and increased mitosis in retinal progenitors. C1P addition advanced photoreceptor differentiation, enhancing opsin and peripherin expression and stimulating development of the apical processes in which these proteins were concentrated. In the absence of these trophic factors, photoreceptors degenerated after 4 days in vitro, and at day 6, almost 50% of photoreceptors were apoptotic. C1P decreased photoreceptor apoptosis, reducing this percentage by half. Inhibiting caspase activity reduced photoreceptor apoptosis in the controls, but did not increase opsin expression, implying that C1P has separate effects on differentiation and survival. <b>Conclusions.</b> These results suggest for the first time that C1P is a novel mediator that has multiple functions in photoreceptors, initially regulating their proliferation and then promoting their survival and differentiation.Instituto Multidisciplinario de Biología Celular2011-07-04info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionArticulohttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdf6580-6588http://sedici.unlp.edu.ar/handle/10915/84041enginfo:eu-repo/semantics/altIdentifier/issn/0146-0404info:eu-repo/semantics/altIdentifier/doi/10.1167/iovs.10-7065info:eu-repo/semantics/openAccesshttp://creativecommons.org/licenses/by-nc-sa/4.0/Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International (CC BY-NC-SA 4.0)reponame:SEDICI (UNLP)instname:Universidad Nacional de La Platainstacron:UNLP2024-05-08T12:54:07Zoai:sedici.unlp.edu.ar:10915/84041Institucionalhttp://sedici.unlp.edu.ar/Universidad públicaNo correspondehttp://sedici.unlp.edu.ar/oai/snrdalira@sedici.unlp.edu.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:13292024-05-08 12:54:07.622SEDICI (UNLP) - Universidad Nacional de La Platafalse |
| dc.title.none.fl_str_mv |
Ceramide-1-phosphate, a new mediator of development and survival in retina photoreceptors |
| title |
Ceramide-1-phosphate, a new mediator of development and survival in retina photoreceptors |
| spellingShingle |
Ceramide-1-phosphate, a new mediator of development and survival in retina photoreceptors Miranda, Gisela E. Biología Sphingolipids Ceramide-1-phosphate Retina Biología Celular |
| title_short |
Ceramide-1-phosphate, a new mediator of development and survival in retina photoreceptors |
| title_full |
Ceramide-1-phosphate, a new mediator of development and survival in retina photoreceptors |
| title_fullStr |
Ceramide-1-phosphate, a new mediator of development and survival in retina photoreceptors |
| title_full_unstemmed |
Ceramide-1-phosphate, a new mediator of development and survival in retina photoreceptors |
| title_sort |
Ceramide-1-phosphate, a new mediator of development and survival in retina photoreceptors |
| dc.creator.none.fl_str_mv |
Miranda, Gisela E. Abrahan, Carolina E. Agnolazza, Daniela L. Politi, Luis E. Rotstein, Nora P. |
| author |
Miranda, Gisela E. |
| author_facet |
Miranda, Gisela E. Abrahan, Carolina E. Agnolazza, Daniela L. Politi, Luis E. Rotstein, Nora P. |
| author_role |
author |
| author2 |
Abrahan, Carolina E. Agnolazza, Daniela L. Politi, Luis E. Rotstein, Nora P. |
| author2_role |
author author author author |
| dc.subject.none.fl_str_mv |
Biología Sphingolipids Ceramide-1-phosphate Retina Biología Celular |
| topic |
Biología Sphingolipids Ceramide-1-phosphate Retina Biología Celular |
| description |
<b>Purpose.</b> Simple sphingolipids control crucial cellular processes in several cell types. Previous work demonstrated that sphingolipids, such as ceramide, sphingosine, and sphingosine-1-phosphate, are key mediators in the regulation of survival, differentiation, and proliferation of retina photoreceptors. Ceramide-1-phosphate (C1P) regulates growth and survival in several cell types; however, little is known concerning its functions in the retina. Whether C1P also participates in controlling photoreceptor development was also explored. <b>Methods.</b> Rat retina neuronal cultures were supplemented with 1 to 10 μM C1P. Proliferation was determined by evaluating 5-bromo-2-deoxyuridine (BrdU) uptake and the number of mitotic figures and differentiation by evaluating opsin and peripherin expression by immunocytochemistry and Western blot. Apoptosis was inhibited with the pan caspase inhibitor ZVADFMK and evaluated by TUNEL assay, propidium iodide/annexin V, and DAPI labeling. Preservation of mitochondrial membrane potential was evaluated. <b>Results.</b> C1P enhanced BrdU uptake and increased mitosis in retinal progenitors. C1P addition advanced photoreceptor differentiation, enhancing opsin and peripherin expression and stimulating development of the apical processes in which these proteins were concentrated. In the absence of these trophic factors, photoreceptors degenerated after 4 days in vitro, and at day 6, almost 50% of photoreceptors were apoptotic. C1P decreased photoreceptor apoptosis, reducing this percentage by half. Inhibiting caspase activity reduced photoreceptor apoptosis in the controls, but did not increase opsin expression, implying that C1P has separate effects on differentiation and survival. <b>Conclusions.</b> These results suggest for the first time that C1P is a novel mediator that has multiple functions in photoreceptors, initially regulating their proliferation and then promoting their survival and differentiation. |
| publishDate |
2011 |
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2011-07-04 |
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info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion Articulo http://purl.org/coar/resource_type/c_6501 info:ar-repo/semantics/articulo |
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article |
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http://sedici.unlp.edu.ar/handle/10915/84041 |
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eng |
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eng |
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info:eu-repo/semantics/altIdentifier/issn/0146-0404 info:eu-repo/semantics/altIdentifier/doi/10.1167/iovs.10-7065 |
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info:eu-repo/semantics/openAccess http://creativecommons.org/licenses/by-nc-sa/4.0/ Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International (CC BY-NC-SA 4.0) |
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openAccess |
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http://creativecommons.org/licenses/by-nc-sa/4.0/ Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International (CC BY-NC-SA 4.0) |
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