Arsenic metabolism in high altitude modern stromatolites revealed by metagenomic analysis

Modern stromatolites thrive only in selected locations in the world. Socompa Lake, located in the Andean plateau at 3570 masl, is one of the numerous extreme Andean microbial ecosystems described over recent years. Extreme environmental conditions include hypersalinity, high UV incidence, and high a...

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Autores: Kurth, Daniel German, Amadio, Ariel F., Ordoñez, Omar F., Allbarracín, Virginia H., Farías, María E., Gärtner, Wolfgang
Tipo de recurso: artículo
Estado:Versión publicada
Fecha de publicación:2017
País:Argentina
Institución:Instituto Nacional de Tecnología Agropecuaria
Repositorio:INTA Digital (INTA)
Idioma:inglés
OAI Identifier:oai:localhost:20.500.12123/630
Acceso en línea:http://hdl.handle.net/20.500.12123/630
https://www.nature.com/articles/s41598-017-00896-0
Access Level:acceso abierto
Palabra clave:Genética
Arsénico
Genómica
Arsenic
Genomics
Genetics
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spelling Arsenic metabolism in high altitude modern stromatolites revealed by metagenomic analysisKurth, Daniel GermanAmadio, Ariel F.Ordoñez, Omar F.Allbarracín, Virginia H.Farías, María E.Gärtner, WolfgangGenéticaArsénicoGenómicaArsenicGenomicsGeneticsModern stromatolites thrive only in selected locations in the world. Socompa Lake, located in the Andean plateau at 3570 masl, is one of the numerous extreme Andean microbial ecosystems described over recent years. Extreme environmental conditions include hypersalinity, high UV incidence, and high arsenic content, among others. After Socompa’s stromatolite microbial communities were analysed by metagenomic DNA sequencing, taxonomic classification showed dominance of Proteobacteria, Bacteroidetes and Firmicutes, and a remarkably high number of unclassified sequences. A functional analysis indicated that carbon fixation might occur not only by the Calvin-Benson cycle, but also through alternative pathways such as the reverse TCA cycle, and the reductive acetyl-CoA pathway. Deltaproteobacteria were involved both in sulfate reduction and nitrogen fixation. Significant differences were found when comparing the Socompa stromatolite metagenome to the Shark Bay (Australia) smooth mat metagenome: namely, those involving stress related processes, particularly, arsenic resistance. An in-depth analysis revealed a surprisingly diverse metabolism comprising all known types of As resistance and energy generating pathways. While the ars operon was the main mechanism, an important abundance of arsM genes was observed in selected phyla. The data resulting from this work will prove a cornerstone for further studies on this rare microbial community.EEA RafaelaFil: Kurth, Daniel German. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Tucumán. Planta Piloto de Procesos Industriales Microbiológicos; ArgentinaFil: Amadio, Ariel F. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Rafaela; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaFil: Ordoñez, Omar F. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Tucumán. Planta Piloto de Procesos Industriales Microbiológicos; ArgentinaFil: Allbarracín, Virginia H. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Tucumán. Planta Piloto de Procesos Industriales Microbiológicos; Argentina. Universidad Nacional de Tucumán. Facultad de Ciencias Naturales. Instituto Miguel Lillo; ArgentinaFil: Farías, María E. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Tucumán. Planta Piloto de Procesos Industriales Microbiológicos; ArgentinaFil: Gärtner, Wolfgang. Max-Planck Institute for Chemical Energy Conversion; Alemania2017-07-10T18:56:51Z2017-07-10T18:56:51Z2017info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfhttp://hdl.handle.net/20.500.12123/630https://www.nature.com/articles/s41598-017-00896-02045-2322Scientific Reports 7, Article number: 1024reponame:INTA Digital (INTA)instname:Instituto Nacional de Tecnología Agropecuariaenginfo:eu-repo/semantics/openAccesshttp://creativecommons.org/licenses/by-nc-sa/4.0/Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International (CC BY-NC-SA 4.0)2024-05-15T12:23:24Zoai:localhost:20.500.12123/630instacron:INTAInstitucionalhttp://repositorio.inta.gob.ar/Organismo científico-tecnológicoNo correspondehttp://repositorio.inta.gob.ar/oai/requesttripaldi.nicolas@inta.gob.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:l2024-05-15 12:23:24.547INTA Digital (INTA) - Instituto Nacional de Tecnología Agropecuariafalse
dc.title.none.fl_str_mv Arsenic metabolism in high altitude modern stromatolites revealed by metagenomic analysis
title Arsenic metabolism in high altitude modern stromatolites revealed by metagenomic analysis
spellingShingle Arsenic metabolism in high altitude modern stromatolites revealed by metagenomic analysis
Kurth, Daniel German
Genética
Arsénico
Genómica
Arsenic
Genomics
Genetics
title_short Arsenic metabolism in high altitude modern stromatolites revealed by metagenomic analysis
title_full Arsenic metabolism in high altitude modern stromatolites revealed by metagenomic analysis
title_fullStr Arsenic metabolism in high altitude modern stromatolites revealed by metagenomic analysis
title_full_unstemmed Arsenic metabolism in high altitude modern stromatolites revealed by metagenomic analysis
title_sort Arsenic metabolism in high altitude modern stromatolites revealed by metagenomic analysis
dc.creator.none.fl_str_mv Kurth, Daniel German
Amadio, Ariel F.
Ordoñez, Omar F.
Allbarracín, Virginia H.
Farías, María E.
Gärtner, Wolfgang
author Kurth, Daniel German
author_facet Kurth, Daniel German
Amadio, Ariel F.
Ordoñez, Omar F.
Allbarracín, Virginia H.
Farías, María E.
Gärtner, Wolfgang
author_role author
author2 Amadio, Ariel F.
Ordoñez, Omar F.
Allbarracín, Virginia H.
Farías, María E.
Gärtner, Wolfgang
author2_role author
author
author
author
author
dc.subject.none.fl_str_mv Genética
Arsénico
Genómica
Arsenic
Genomics
Genetics
topic Genética
Arsénico
Genómica
Arsenic
Genomics
Genetics
description Modern stromatolites thrive only in selected locations in the world. Socompa Lake, located in the Andean plateau at 3570 masl, is one of the numerous extreme Andean microbial ecosystems described over recent years. Extreme environmental conditions include hypersalinity, high UV incidence, and high arsenic content, among others. After Socompa’s stromatolite microbial communities were analysed by metagenomic DNA sequencing, taxonomic classification showed dominance of Proteobacteria, Bacteroidetes and Firmicutes, and a remarkably high number of unclassified sequences. A functional analysis indicated that carbon fixation might occur not only by the Calvin-Benson cycle, but also through alternative pathways such as the reverse TCA cycle, and the reductive acetyl-CoA pathway. Deltaproteobacteria were involved both in sulfate reduction and nitrogen fixation. Significant differences were found when comparing the Socompa stromatolite metagenome to the Shark Bay (Australia) smooth mat metagenome: namely, those involving stress related processes, particularly, arsenic resistance. An in-depth analysis revealed a surprisingly diverse metabolism comprising all known types of As resistance and energy generating pathways. While the ars operon was the main mechanism, an important abundance of arsM genes was observed in selected phyla. The data resulting from this work will prove a cornerstone for further studies on this rare microbial community.
publishDate 2017
dc.date.none.fl_str_mv 2017-07-10T18:56:51Z
2017-07-10T18:56:51Z
2017
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/20.500.12123/630
https://www.nature.com/articles/s41598-017-00896-0
2045-2322
url http://hdl.handle.net/20.500.12123/630
https://www.nature.com/articles/s41598-017-00896-0
identifier_str_mv 2045-2322
dc.language.none.fl_str_mv eng
language eng
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
http://creativecommons.org/licenses/by-nc-sa/4.0/
Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International (CC BY-NC-SA 4.0)
eu_rights_str_mv openAccess
rights_invalid_str_mv http://creativecommons.org/licenses/by-nc-sa/4.0/
Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International (CC BY-NC-SA 4.0)
dc.format.none.fl_str_mv application/pdf
dc.source.none.fl_str_mv Scientific Reports 7, Article number: 1024
reponame:INTA Digital (INTA)
instname:Instituto Nacional de Tecnología Agropecuaria
instname_str Instituto Nacional de Tecnología Agropecuaria
reponame_str INTA Digital (INTA)
collection INTA Digital (INTA)
repository.name.fl_str_mv INTA Digital (INTA) - Instituto Nacional de Tecnología Agropecuaria
repository.mail.fl_str_mv tripaldi.nicolas@inta.gob.ar
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