Physiological and genotoxic effects of Molybdenum-induced copper deficiency in cattle

Molybdenosis is a disease caused by the depressing effect of molybdenumn (Mo) on the physiological availability of Cu. The present study was carried out in order to analyze the ability of Mo to cause damage on the DNA integrity and changes in membrane fatty acids by oxidative damage. Holstein male c...

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Autores: Picco, Sebastian Julio, Ponzzinibio, María Virginia, Mattioli, Guillermo Alberto, Rosa, Diana Esther, Minatel, Leonardo, Fazzio, Luis Emilio, Seoane, Analía Isabel
Tipo de recurso: artículo
Estado:Versión publicada
Fecha de publicación:2012
País:Argentina
Institución:Universidad Nacional de La Plata
Repositorio:SEDICI (UNLP)
Idioma:inglés
OAI Identifier:oai:sedici.unlp.edu.ar:10915/99409
Acceso en línea:http://sedici.unlp.edu.ar/handle/10915/99409
Access Level:acceso abierto
Palabra clave:Ciencias Veterinarias
Genotoxicity
Molibdenum
Copper deficiency
Oxidative damage
DNA damage
Bos taurus
SOD activity
Molibdeno
Deficiencia de cobre
Daño oxidativo
Daño del ADN
Actividad SOD
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dc.title.none.fl_str_mv Physiological and genotoxic effects of Molybdenum-induced copper deficiency in cattle
Efecto fisiológico y genotóxico de la deficiencia de cobre inducida por Molibdeno en bovinos
title Physiological and genotoxic effects of Molybdenum-induced copper deficiency in cattle
spellingShingle Physiological and genotoxic effects of Molybdenum-induced copper deficiency in cattle
Picco, Sebastian Julio
Ciencias Veterinarias
Genotoxicity
Molibdenum
Copper deficiency
Oxidative damage
DNA damage
Bos taurus
SOD activity
Molibdeno
Deficiencia de cobre
Daño oxidativo
Daño del ADN
Bos taurus
Actividad SOD
title_short Physiological and genotoxic effects of Molybdenum-induced copper deficiency in cattle
title_full Physiological and genotoxic effects of Molybdenum-induced copper deficiency in cattle
title_fullStr Physiological and genotoxic effects of Molybdenum-induced copper deficiency in cattle
title_full_unstemmed Physiological and genotoxic effects of Molybdenum-induced copper deficiency in cattle
title_sort Physiological and genotoxic effects of Molybdenum-induced copper deficiency in cattle
dc.creator.none.fl_str_mv Picco, Sebastian Julio
Ponzzinibio, María Virginia
Mattioli, Guillermo Alberto
Rosa, Diana Esther
Minatel, Leonardo
Fazzio, Luis Emilio
Seoane, Analía Isabel
author Picco, Sebastian Julio
author_facet Picco, Sebastian Julio
Ponzzinibio, María Virginia
Mattioli, Guillermo Alberto
Rosa, Diana Esther
Minatel, Leonardo
Fazzio, Luis Emilio
Seoane, Analía Isabel
author_role author
author2 Ponzzinibio, María Virginia
Mattioli, Guillermo Alberto
Rosa, Diana Esther
Minatel, Leonardo
Fazzio, Luis Emilio
Seoane, Analía Isabel
author2_role author
author
author
author
author
author
dc.subject.none.fl_str_mv Ciencias Veterinarias
Genotoxicity
Molibdenum
Copper deficiency
Oxidative damage
DNA damage
Bos taurus
SOD activity
Molibdeno
Deficiencia de cobre
Daño oxidativo
Daño del ADN
Bos taurus
Actividad SOD
topic Ciencias Veterinarias
Genotoxicity
Molibdenum
Copper deficiency
Oxidative damage
DNA damage
Bos taurus
SOD activity
Molibdeno
Deficiencia de cobre
Daño oxidativo
Daño del ADN
Bos taurus
Actividad SOD
description Molybdenosis is a disease caused by the depressing effect of molybdenumn (Mo) on the physiological availability of Cu. The present study was carried out in order to analyze the ability of Mo to cause damage on the DNA integrity and changes in membrane fatty acids by oxidative damage. Holstein male calves were fed a Mo-supplemented diet for 9 months. Variables evaluated were plasma Cu concentration, erythrocyte Cu content and SOD activity, comet assay and analysis of the fatty acid composition of erythrocyte membranes. The statistical design was a completely randomized with one single factor and two replications. Copper plasma concentration, erythrocyte copper concentration and Cu/Zn SOD activity were analyzed using the t test. Chi-square test was used to compare the number of cells with DNA damage and one-way analysis of variance and Tukey test (p≤0.05) for fatty acid composition and lipid peroxidation. Results showed that Mo in the diet induced a depletion of hepatic Cu storage, a decrease of Cu plasma and erythrocyte levels, a fall in Cu/Zn-SOD activity, changes in membrane fatty acids composition and DNA damage. These results are in agreement with the three phases model of copper deficiency and validate the occurrence of molybdenosis or secondary hypocuprosis. Further studies will be necessary to explore the mechanisms involved in the DNA damage and to distinguish primary molybdenum toxicosis from the molybdenum-induced copper deficiency.
publishDate 2012
dc.date.none.fl_str_mv 2012-02
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info:eu-repo/semantics/publishedVersion
Articulo
http://purl.org/coar/resource_type/c_6501
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status_str publishedVersion
dc.identifier.none.fl_str_mv http://sedici.unlp.edu.ar/handle/10915/99409
url http://sedici.unlp.edu.ar/handle/10915/99409
dc.language.none.fl_str_mv eng
language eng
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info:eu-repo/semantics/altIdentifier/url/https://agrociencia-colpos.mx/index.php/agrociencia/article/view/940
info:eu-repo/semantics/altIdentifier/issn/1405-3195
info:eu-repo/semantics/altIdentifier/hdl/11336/11703
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
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Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International (CC BY-NC-SA 4.0)
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dc.source.none.fl_str_mv reponame:SEDICI (UNLP)
instname:Universidad Nacional de La Plata
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spelling Physiological and genotoxic effects of Molybdenum-induced copper deficiency in cattleEfecto fisiológico y genotóxico de la deficiencia de cobre inducida por Molibdeno en bovinosPicco, Sebastian JulioPonzzinibio, María VirginiaMattioli, Guillermo AlbertoRosa, Diana EstherMinatel, LeonardoFazzio, Luis EmilioSeoane, Analía IsabelCiencias VeterinariasGenotoxicityMolibdenumCopper deficiencyOxidative damageDNA damageBos taurusSOD activityMolibdenoDeficiencia de cobreDaño oxidativoDaño del ADNBos taurusActividad SODMolybdenosis is a disease caused by the depressing effect of molybdenumn (Mo) on the physiological availability of Cu. The present study was carried out in order to analyze the ability of Mo to cause damage on the DNA integrity and changes in membrane fatty acids by oxidative damage. Holstein male calves were fed a Mo-supplemented diet for 9 months. Variables evaluated were plasma Cu concentration, erythrocyte Cu content and SOD activity, comet assay and analysis of the fatty acid composition of erythrocyte membranes. The statistical design was a completely randomized with one single factor and two replications. Copper plasma concentration, erythrocyte copper concentration and Cu/Zn SOD activity were analyzed using the t test. Chi-square test was used to compare the number of cells with DNA damage and one-way analysis of variance and Tukey test (p≤0.05) for fatty acid composition and lipid peroxidation. Results showed that Mo in the diet induced a depletion of hepatic Cu storage, a decrease of Cu plasma and erythrocyte levels, a fall in Cu/Zn-SOD activity, changes in membrane fatty acids composition and DNA damage. These results are in agreement with the three phases model of copper deficiency and validate the occurrence of molybdenosis or secondary hypocuprosis. Further studies will be necessary to explore the mechanisms involved in the DNA damage and to distinguish primary molybdenum toxicosis from the molybdenum-induced copper deficiency.La molibdenosis es una enfermedad causada por el efecto depresivo del molibdeno (Mo) en la disponibilidad fisioló- gica del Cu. El presente estudio se llevó a cabo para analizar la capacidad del Mo para causar daño en la integridad del ADN y cambios en los ácidos grasos de la membrana a causa de daño oxidativo. Se alimentaron becerros machos Holstein con una dieta suplementada con Mo durante 9 meses. Las variables evaluadas fueron la concentración de Cu en el plasma, el contenido de Cu en eritrocitos y la actividad SOD, un ensayo cometa y el análisis de la composición de ácidos grasos de las membranas de eritrocitos. El diseño estadístico fue completamente al azar con un solo factor y dos réplicas. Se analizaron la concentración de cobre en el plasma, la concentración de cobre en eritrocitos y la actividad Cu/Zn-SOD con la prueba de t. Se utilizó la prueba de Chi cuadrado para comparar el número de células con daño del ADN y el análisis de varianza en un sentido y la prueba de Tukey (p≤0.05) para la composición de ácidos grasos y la peroxidación de lípidos. Los resultados mostraron que el Mo en la dieta indujo una reducción en el almacenaje hepático de Cu, una disminución de los niveles de Cu en el plasma y eritrocitos, una caída en la actividad Cu/Zn-SOD, cambios en la composición de ácidos grasos de la membrana y daño del ADN. Estos resultados coinciden con el modelo de tres fases de deficiencia del cobre y validan la ocurrencia de molibdenosis o hipocuprosis secundaria. Serán necesarios más estudios para explorar los mecanismos involucrados en el daño del ADN y distinguir la toxicosis primaria por molibdeno a partir de la deficiencia de cobre inducida por el mismo.Instituto de Genética VeterinariaFacultad de Ciencias Veterinarias2012-02info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionArticulohttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdf107-117http://sedici.unlp.edu.ar/handle/10915/99409enginfo:eu-repo/semantics/altIdentifier/url/https://ri.conicet.gov.ar/11336/11703info:eu-repo/semantics/altIdentifier/url/https://agrociencia-colpos.mx/index.php/agrociencia/article/view/940info:eu-repo/semantics/altIdentifier/issn/1405-3195info:eu-repo/semantics/altIdentifier/hdl/11336/11703info:eu-repo/semantics/openAccesshttp://creativecommons.org/licenses/by-nc-sa/4.0/Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International (CC BY-NC-SA 4.0)reponame:SEDICI (UNLP)instname:Universidad Nacional de La Platainstacron:UNLP2024-05-08T12:57:49Zoai:sedici.unlp.edu.ar:10915/99409Institucionalhttp://sedici.unlp.edu.ar/Universidad públicaNo correspondehttp://sedici.unlp.edu.ar/oai/snrdalira@sedici.unlp.edu.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:13292024-05-08 12:57:49.818SEDICI (UNLP) - Universidad Nacional de La Platafalse
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