Complex formation of chlorhexidine gluconate with hydroxypropyl-β- cyclodextrin (HPβCD) by proton nuclear magnetic resonance spectroscopy (1H NMR)
The complex formation of chlorhexidine digluconate (CHX-G2) with hydroxypropyl-β-cyclodextrin (HPβCD) was studied using NMR spectroscopy. The results revealed that this surfactant agent shows an monomer/aggregate equilibrium, which is dependent on the concentration of this drug. This equilibrium can...
| Autores: | , , |
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| Tipo de recurso: | artículo |
| Estado: | Versión publicada |
| Fecha de publicación: | 2011 |
| País: | Argentina |
| Institución: | Consejo Nacional de Investigaciones Científicas y Técnicas |
| Repositorio: | CONICET Digital (CONICET) |
| Idioma: | inglés |
| OAI Identifier: | oai:ri.conicet.gov.ar:11336/190861 |
| Acceso en línea: | http://hdl.handle.net/11336/190861 |
| Access Level: | acceso abierto |
| Palabra clave: | AGGREGATION EQUILIBRIUM CHLORHEXIDINE DIGLUCONATE HYDROXYPROPYL-Β-CYCLODEXTRIN NMR SPECTROSCOPY https://purl.org/becyt/ford/3.3 https://purl.org/becyt/ford/3 |
| Sumario: | The complex formation of chlorhexidine digluconate (CHX-G2) with hydroxypropyl-β-cyclodextrin (HPβCD) was studied using NMR spectroscopy. The results revealed that this surfactant agent shows an monomer/aggregate equilibrium, which is dependent on the concentration of this drug. This equilibrium can be modified by the presence of HPβCD, which reduces the aggregation of the CHX-G2 molecules. An inclusion process of the CHX-G2 aromatic residue within the cyclodextrin cavity was confirmed by 2D ROESY spectroscopy. 1H NMR titration studies of CHX-G2 with HPβCD in D2O confirmed the formation of higher order complexes between CHX-G2 and HPβCD. Moreover, the addition of HPβCD into CHX-G2 solutions forms insoluble aggregates. Such insoluble aggregates may result in the stacking of CHX-G 2 molecules on the surface of the CHX-G2:HPβCD complexes. |
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