Chlamydia trachomatis infection leads to defined alterations to the lipid droplet proteome in epithelial cells
The obligate intracellular bacterium Chlamydia trachomatisis a major human pathogen and a main cause of genital and ocular diseases. During its intracellular cycle, C. trachomatisreplicates inside a membrane-bound vacuole termed an "inclusion". Acquisition of lipids (and other nutrients) f...
| Autores: | , , , , , , |
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| Tipo de recurso: | artículo |
| Estado: | Versión publicada |
| Fecha de publicación: | 2015 |
| País: | Argentina |
| Institución: | Consejo Nacional de Investigaciones Científicas y Técnicas |
| Repositorio: | CONICET Digital (CONICET) |
| Idioma: | inglés |
| OAI Identifier: | oai:ri.conicet.gov.ar:11336/59274 |
| Acceso en línea: | http://hdl.handle.net/11336/59274 |
| Access Level: | acceso abierto |
| Palabra clave: | CHLAMYDIA TRACHOMATIS LIPID DROPLETS HOST PATHOGEN INTERACTIONS QUANTITATIVE PROTEOMICS https://purl.org/becyt/ford/1.6 https://purl.org/becyt/ford/1 |
| Sumario: | The obligate intracellular bacterium Chlamydia trachomatisis a major human pathogen and a main cause of genital and ocular diseases. During its intracellular cycle, C. trachomatisreplicates inside a membrane-bound vacuole termed an "inclusion". Acquisition of lipids (and other nutrients) from the host cell is a critical step in chlamydial replication. Lipid droplets (LD) are ubiquitous, ER-derived neutral lipid-rich storage organelles surrounded by a phospholipids monolayer and associated proteins. Previous studies have shown that LDs accumulate at the periphery of, and eventually translocate into, the chlamydial inclusion. These observations point out to Chlamydia-mediated manipulation of LDs in infected cells, which may impact the function and thereby the protein composition of these organelles. By means of a label-free quantitative mass spectrometry approach we found that the LD proteome is modified in the context of C. trachomatis infection. We determined that LDs isolated from C. trachomatis- infected cells were enriched in proteins related to lipid metabolism, biosynthesis and LD-specific functions. Interestingly, consistent with the observation that LDs intimately associate with the inclusion, a subset of inclusion membrane proteins co-purified with LD protein extracts. Finally, genetic ablation of LDs negatively affected generation of C. trachomatis infectious progeny, consistent with a role for LD biogenesis in optimal chlamydial growth. |
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