Isolation, identification and usefulness of antifungal compounds from Zuccagnia punctata for control of toxigenic ear rot pathogens

Infusion, tincture and decoction of leaves of Zuccagnia punctata Cav. were assayed on growth of Fusarium verticillioides, F. graminearum sensu stricto, F. boothii, F. meridionale, F. subglutinans and F. thapsinum. The tincture showed the lowest IC50 on mycelial growth. A diethyl ether fraction of th...

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Detalhes bibliográficos
Autores: Jiménez, Cristina Marisol, Sampietro, Diego Alejandro, Sgariglia, Melina Araceli, Soberon, Jose Rodolfo, Vattuone, Marta Amelia
Tipo de documento: artigo
Estado:Versão publicada
Data de publicação:2014
País:Argentina
Recursos:Consejo Nacional de Investigaciones Científicas y Técnicas
Repositório:CONICET Digital (CONICET)
Idioma:inglês
OAI Identifier:oai:ri.conicet.gov.ar:11336/12426
Acesso em linha:http://hdl.handle.net/11336/12426
Access Level:Acceso aberto
Palavra-chave:Antifungal
Chalcones
Fusarium
Zuccagnia Punctata
https://purl.org/becyt/ford/4.4
https://purl.org/becyt/ford/4
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repository_id_str
spelling Isolation, identification and usefulness of antifungal compounds from Zuccagnia punctata for control of toxigenic ear rot pathogensJiménez, Cristina MarisolSampietro, Diego AlejandroSgariglia, Melina AraceliSoberon, Jose RodolfoVattuone, Marta AmeliaAntifungalChalconesFusariumZuccagnia Punctatahttps://purl.org/becyt/ford/4.4https://purl.org/becyt/ford/4Infusion, tincture and decoction of leaves of Zuccagnia punctata Cav. were assayed on growth of Fusarium verticillioides, F. graminearum sensu stricto, F. boothii, F. meridionale, F. subglutinans and F. thapsinum. The tincture showed the lowest IC50 on mycelial growth. A diethyl ether fraction of the tincture showed the highest antifungal activity in microdilution assays on F. verticillioides and F. graminearum. The antifungal constituents were separated by silica gel chromatography and identified as 2´,4´-dihydroxychalcone, 2´,4´-dihydroxy-3´-methoxychalcone and 7-hydroxy-3´,4´-dimethoxyflavone. These chalcones had the lowest MIC and MFC values on F. verticillioides and F. graminearum sensu stricto. 2´,4´-Dihydroxychalcone was mildly toxic and the remaining identified compounds were non-toxic in the brine shrimp assay. 2´,4´-Dihydroxychalcone in mixtures with commercial food preservatives showed additive effects on F. graminearum sensu stricto and synergistic ones on F. verticillioides. 2´,4´-Dihydroxy-3´-methoxychalcone showed synergistic effects in mixtures. Our results suggest that addition of chalcones to food preservatives allows reduction in the doses of the preservatives required for control of Fusarium species.Fil: Jiménez, Cristina Marisol. Universidad Nacional de Tucumán. Facultad de Bioquímica, Química y Farmacia; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaFil: Sampietro, Diego Alejandro. Universidad Nacional de Tucumán. Facultad de Bioquímica, Química y Farmacia; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaFil: Sgariglia, Melina Araceli. Universidad Nacional de Tucumán. Facultad de Bioquímica, Química y Farmacia; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaFil: Soberon, Jose Rodolfo. Universidad Nacional de Tucumán. Facultad de Bioquímica, Química y Farmacia; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaFil: Vattuone, Marta Amelia. Universidad Nacional de Tucumán. Facultad de Bioquímica, Química y Farmacia; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaNatural Products2014-10info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/12426Jiménez, Cristina Marisol; Sampietro, Diego Alejandro; Sgariglia, Melina Araceli; Soberon, Jose Rodolfo; Vattuone, Marta Amelia; Isolation, identification and usefulness of antifungal compounds from Zuccagnia punctata for control of toxigenic ear rot pathogens; Natural Products; Natural Product Communications; 9; 10; 10-2014; 1461-14641934-578X1555-9475enginfo:eu-repo/semantics/altIdentifier/url/https://journals.sagepub.com/doi/abs/10.1177/1934578X1400901014info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2024-05-08T13:59:00Zoai:ri.conicet.gov.ar:11336/12426instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982024-05-08 13:59:01.156CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Isolation, identification and usefulness of antifungal compounds from Zuccagnia punctata for control of toxigenic ear rot pathogens
title Isolation, identification and usefulness of antifungal compounds from Zuccagnia punctata for control of toxigenic ear rot pathogens
spellingShingle Isolation, identification and usefulness of antifungal compounds from Zuccagnia punctata for control of toxigenic ear rot pathogens
Jiménez, Cristina Marisol
Antifungal
Chalcones
Fusarium
Zuccagnia Punctata
https://purl.org/becyt/ford/4.4
https://purl.org/becyt/ford/4
title_short Isolation, identification and usefulness of antifungal compounds from Zuccagnia punctata for control of toxigenic ear rot pathogens
title_full Isolation, identification and usefulness of antifungal compounds from Zuccagnia punctata for control of toxigenic ear rot pathogens
title_fullStr Isolation, identification and usefulness of antifungal compounds from Zuccagnia punctata for control of toxigenic ear rot pathogens
title_full_unstemmed Isolation, identification and usefulness of antifungal compounds from Zuccagnia punctata for control of toxigenic ear rot pathogens
title_sort Isolation, identification and usefulness of antifungal compounds from Zuccagnia punctata for control of toxigenic ear rot pathogens
dc.creator.none.fl_str_mv Jiménez, Cristina Marisol
Sampietro, Diego Alejandro
Sgariglia, Melina Araceli
Soberon, Jose Rodolfo
Vattuone, Marta Amelia
author Jiménez, Cristina Marisol
author_facet Jiménez, Cristina Marisol
Sampietro, Diego Alejandro
Sgariglia, Melina Araceli
Soberon, Jose Rodolfo
Vattuone, Marta Amelia
author_role author
author2 Sampietro, Diego Alejandro
Sgariglia, Melina Araceli
Soberon, Jose Rodolfo
Vattuone, Marta Amelia
author2_role author
author
author
author
dc.subject.none.fl_str_mv Antifungal
Chalcones
Fusarium
Zuccagnia Punctata
https://purl.org/becyt/ford/4.4
https://purl.org/becyt/ford/4
topic Antifungal
Chalcones
Fusarium
Zuccagnia Punctata
https://purl.org/becyt/ford/4.4
https://purl.org/becyt/ford/4
description Infusion, tincture and decoction of leaves of Zuccagnia punctata Cav. were assayed on growth of Fusarium verticillioides, F. graminearum sensu stricto, F. boothii, F. meridionale, F. subglutinans and F. thapsinum. The tincture showed the lowest IC50 on mycelial growth. A diethyl ether fraction of the tincture showed the highest antifungal activity in microdilution assays on F. verticillioides and F. graminearum. The antifungal constituents were separated by silica gel chromatography and identified as 2´,4´-dihydroxychalcone, 2´,4´-dihydroxy-3´-methoxychalcone and 7-hydroxy-3´,4´-dimethoxyflavone. These chalcones had the lowest MIC and MFC values on F. verticillioides and F. graminearum sensu stricto. 2´,4´-Dihydroxychalcone was mildly toxic and the remaining identified compounds were non-toxic in the brine shrimp assay. 2´,4´-Dihydroxychalcone in mixtures with commercial food preservatives showed additive effects on F. graminearum sensu stricto and synergistic ones on F. verticillioides. 2´,4´-Dihydroxy-3´-methoxychalcone showed synergistic effects in mixtures. Our results suggest that addition of chalcones to food preservatives allows reduction in the doses of the preservatives required for control of Fusarium species.
publishDate 2014
dc.date.none.fl_str_mv 2014-10
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/12426
Jiménez, Cristina Marisol; Sampietro, Diego Alejandro; Sgariglia, Melina Araceli; Soberon, Jose Rodolfo; Vattuone, Marta Amelia; Isolation, identification and usefulness of antifungal compounds from Zuccagnia punctata for control of toxigenic ear rot pathogens; Natural Products; Natural Product Communications; 9; 10; 10-2014; 1461-1464
1934-578X
1555-9475
url http://hdl.handle.net/11336/12426
identifier_str_mv Jiménez, Cristina Marisol; Sampietro, Diego Alejandro; Sgariglia, Melina Araceli; Soberon, Jose Rodolfo; Vattuone, Marta Amelia; Isolation, identification and usefulness of antifungal compounds from Zuccagnia punctata for control of toxigenic ear rot pathogens; Natural Products; Natural Product Communications; 9; 10; 10-2014; 1461-1464
1934-578X
1555-9475
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/url/https://journals.sagepub.com/doi/abs/10.1177/1934578X1400901014
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
dc.publisher.none.fl_str_mv Natural Products
publisher.none.fl_str_mv Natural Products
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
_version_ 1799195642718846976
score 15.812429

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