Membrane localization of Junín virus glycoproteins requires cholesterol and cholesterol rich membranes

Arenavirus morphogenesis and budding occurs at cellular plasma membrane;however, the nature of membrane assembly sites remains poorly understood. In this study we examined the effect of different cholesterol-lowering agents on Junín virus (JUNV) multiplication. We found that cholesterol cell depleti...

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Authors: Cordo, Sandra Myriam, Valko, Ayelén, Martinez, María Guadalupe, Candurra, Nélida Alicia
Format: article
Status:Published version
Publication Date:2013
Country:Argentina
Institution:Consejo Nacional de Investigaciones Científicas y Técnicas
Repository:CONICET Digital (CONICET)
Language:English
OAI Identifier:oai:ri.conicet.gov.ar:11336/66284
Online Access:http://hdl.handle.net/11336/66284
Access Level:Open access
Keyword:Cholesterol
Junv
Membrane
Glycoprotein
https://purl.org/becyt/ford/1.6
https://purl.org/becyt/ford/1
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network_name_str Argentina
repository_id_str
spelling Membrane localization of Junín virus glycoproteins requires cholesterol and cholesterol rich membranesCordo, Sandra MyriamValko, AyelénMartinez, María GuadalupeCandurra, Nélida AliciaCholesterolJunvMembraneGlycoproteinhttps://purl.org/becyt/ford/1.6https://purl.org/becyt/ford/1Arenavirus morphogenesis and budding occurs at cellular plasma membrane;however, the nature of membrane assembly sites remains poorly understood. In this study we examined the effect of different cholesterol-lowering agents on Junín virus (JUNV) multiplication. We found that cholesterol cell depletion reduced JUNV glycoproteins (GPs) membrane expression and virus budding. Analysis of membrane protein insolubility in Triton X-100 suggested that JUNV GPs associate with cholesterol enriched membranes. Rafts dissociation conditions as warm detergent extraction and cholesterol removal by methyl-b-cyclodextrin compound showed to impair GPs cholesterol enriched membrane association.Analysis of GPs transfected cells showed similar results suggesting that membrane raft association is independent of other viral proteins.Fil: Cordo, Sandra Myriam. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Ciudad Universitaria. Instituto de Química Biológica de la Facultad de Ciencias Exactas y Naturales. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Instituto de Química Biológica de la Facultad de Ciencias Exactas y Naturales; ArgentinaFil: Valko, Ayelén. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Ciudad Universitaria. Instituto de Química Biológica de la Facultad de Ciencias Exactas y Naturales. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Instituto de Química Biológica de la Facultad de Ciencias Exactas y Naturales; ArgentinaFil: Martinez, María Guadalupe. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Ciudad Universitaria. Instituto de Química Biológica de la Facultad de Ciencias Exactas y Naturales. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Instituto de Química Biológica de la Facultad de Ciencias Exactas y Naturales; ArgentinaFil: Candurra, Nélida Alicia. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Ciudad Universitaria. Instituto de Química Biológica de la Facultad de Ciencias Exactas y Naturales. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Instituto de Química Biológica de la Facultad de Ciencias Exactas y Naturales; ArgentinaAcademic Press Inc Elsevier Science2013-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfapplication/pdfapplication/pdfapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/66284Cordo, Sandra Myriam; Valko, Ayelén; Martinez, María Guadalupe; Candurra, Nélida Alicia; Membrane localization of Junín virus glycoproteins requires cholesterol and cholesterol rich membranes; Academic Press Inc Elsevier Science; Biochemical and Biophysical Research Communications; 430; 3; 1-2013; 912-9170006-291XCONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/doi/10.1016/j.bbrc.2012.12.053info:eu-repo/semantics/altIdentifier/url/https://www.sciencedirect.com/science/article/pii/S0006291X12024011info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2024-05-08T13:48:31Zoai:ri.conicet.gov.ar:11336/66284instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982024-05-08 13:48:31.729CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Membrane localization of Junín virus glycoproteins requires cholesterol and cholesterol rich membranes
title Membrane localization of Junín virus glycoproteins requires cholesterol and cholesterol rich membranes
spellingShingle Membrane localization of Junín virus glycoproteins requires cholesterol and cholesterol rich membranes
Cordo, Sandra Myriam
Cholesterol
Junv
Membrane
Glycoprotein
https://purl.org/becyt/ford/1.6
https://purl.org/becyt/ford/1
title_short Membrane localization of Junín virus glycoproteins requires cholesterol and cholesterol rich membranes
title_full Membrane localization of Junín virus glycoproteins requires cholesterol and cholesterol rich membranes
title_fullStr Membrane localization of Junín virus glycoproteins requires cholesterol and cholesterol rich membranes
title_full_unstemmed Membrane localization of Junín virus glycoproteins requires cholesterol and cholesterol rich membranes
title_sort Membrane localization of Junín virus glycoproteins requires cholesterol and cholesterol rich membranes
dc.creator.none.fl_str_mv Cordo, Sandra Myriam
Valko, Ayelén
Martinez, María Guadalupe
Candurra, Nélida Alicia
author Cordo, Sandra Myriam
author_facet Cordo, Sandra Myriam
Valko, Ayelén
Martinez, María Guadalupe
Candurra, Nélida Alicia
author_role author
author2 Valko, Ayelén
Martinez, María Guadalupe
Candurra, Nélida Alicia
author2_role author
author
author
dc.subject.none.fl_str_mv Cholesterol
Junv
Membrane
Glycoprotein
https://purl.org/becyt/ford/1.6
https://purl.org/becyt/ford/1
topic Cholesterol
Junv
Membrane
Glycoprotein
https://purl.org/becyt/ford/1.6
https://purl.org/becyt/ford/1
description Arenavirus morphogenesis and budding occurs at cellular plasma membrane;however, the nature of membrane assembly sites remains poorly understood. In this study we examined the effect of different cholesterol-lowering agents on Junín virus (JUNV) multiplication. We found that cholesterol cell depletion reduced JUNV glycoproteins (GPs) membrane expression and virus budding. Analysis of membrane protein insolubility in Triton X-100 suggested that JUNV GPs associate with cholesterol enriched membranes. Rafts dissociation conditions as warm detergent extraction and cholesterol removal by methyl-b-cyclodextrin compound showed to impair GPs cholesterol enriched membrane association.Analysis of GPs transfected cells showed similar results suggesting that membrane raft association is independent of other viral proteins.
publishDate 2013
dc.date.none.fl_str_mv 2013-01
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/66284
Cordo, Sandra Myriam; Valko, Ayelén; Martinez, María Guadalupe; Candurra, Nélida Alicia; Membrane localization of Junín virus glycoproteins requires cholesterol and cholesterol rich membranes; Academic Press Inc Elsevier Science; Biochemical and Biophysical Research Communications; 430; 3; 1-2013; 912-917
0006-291X
CONICET Digital
CONICET
url http://hdl.handle.net/11336/66284
identifier_str_mv Cordo, Sandra Myriam; Valko, Ayelén; Martinez, María Guadalupe; Candurra, Nélida Alicia; Membrane localization of Junín virus glycoproteins requires cholesterol and cholesterol rich membranes; Academic Press Inc Elsevier Science; Biochemical and Biophysical Research Communications; 430; 3; 1-2013; 912-917
0006-291X
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/doi/10.1016/j.bbrc.2012.12.053
info:eu-repo/semantics/altIdentifier/url/https://www.sciencedirect.com/science/article/pii/S0006291X12024011
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
application/pdf
application/pdf
application/pdf
application/pdf
dc.publisher.none.fl_str_mv Academic Press Inc Elsevier Science
publisher.none.fl_str_mv Academic Press Inc Elsevier Science
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
_version_ 1799195300702715904
score 15,812429

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