Lack of operant ethanol self-administration in dopamine D2 receptor knockout mice

Rationale: Dopamine D2 receptors are postulated to play an important role in modulating the reinforcing effects of abused drugs including ethanol. Objectives: This experiment examined operant ethanol self-administration in dopamine D2 receptor knockout (KO) mice and wild-type (WT) mice using a conti...

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Autores: Risinger, Fred O., Freeman, Pierre A., Rubinstein, Marcelo, Low, Malcolm J., Grandy, David K.
Tipo de recurso: artículo
Estado:Versión publicada
Fecha de publicación:2000
País:Argentina
Institución:Consejo Nacional de Investigaciones Científicas y Técnicas
Repositorio:CONICET Digital (CONICET)
Idioma:inglés
OAI Identifier:oai:ri.conicet.gov.ar:11336/71734
Acceso en línea:http://hdl.handle.net/11336/71734
Access Level:acceso abierto
Palabra clave:Continuous Access
Dopamine D2 Receptor Knockout Mice
Ethanol Self-Administration
Reinforcement
https://purl.org/becyt/ford/3.1
https://purl.org/becyt/ford/3
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network_acronym_str AR
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repository_id_str
spelling Lack of operant ethanol self-administration in dopamine D2 receptor knockout miceRisinger, Fred O.Freeman, Pierre A.Rubinstein, MarceloLow, Malcolm J.Grandy, David K.Continuous AccessDopamine D2 Receptor Knockout MiceEthanol Self-AdministrationReinforcementhttps://purl.org/becyt/ford/3.1https://purl.org/becyt/ford/3Rationale: Dopamine D2 receptors are postulated to play an important role in modulating the reinforcing effects of abused drugs including ethanol. Objectives: This experiment examined operant ethanol self-administration in dopamine D2 receptor knockout (KO) mice and wild-type (WT) mice using a continuous access procedure. Methods: Adult male KO and WT mice were trained in 30-min sessions to perform a lever press response for access to 10% v/v ethanol. After training, the mice were placed in test chambers on a continuous (23 h/day) basis with access to food (one lever press, i.e., FR1), 10% v/v ethanol (four lever presses, i.e., FR4), and water from a sipper tube (phase 1). After 30 consecutive sessions, response patterns were determined for 0, 5, 10, 20 and 30% v/v ethanol (phase 2). Saccharin (0.2% w/v) was subsequently added to the ethanol mixture and responding was examined for 0, 5, 10 and 20% ethanol (phase 3). Results: During phase 1, WT mice displayed higher ethanol-lever responding compared to KO mice. Food lever responding and water intake was the same in both genotypes. During phase 2, WT mice displayed concentration-dependent ethanol lever responding, whereas KO mice responded at low rates regardless of ethanol concentration. WT mice also responded more for food compared to KO mice. Each genotype showed similar water intakes except at the 20% ethanol concentration, where WT mice had lower intakes. During phase 3, WT mice continued to show higher responding for all concentrations including saccharin alone. WT mice also continued to respond more for food compared to KO mice, but drank less water. In each phase, WT mice displayed episodic (bout) responding on the ethanol lever. KO mice did not respond for ethanol in bouts. Conclusions: Reduced responding in the KO mice for several reinforcers including ethanol indicates a more general role for dopamine D2 receptors in motivated responding rather than a specific role in ethanol reinforcement.Fil: Risinger, Fred O.. Oregon Health Sciences University; Estados UnidosFil: Freeman, Pierre A.. Oregon Health Sciences University; Estados UnidosFil: Rubinstein, Marcelo. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Investigaciones en Ingeniería Genética y Biología Molecular "Dr. Héctor N. Torres"; ArgentinaFil: Low, Malcolm J.. Oregon Health Sciences University; Estados UnidosFil: Grandy, David K.. Oregon Health Sciences University; Estados UnidosSpringer2000-10info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/71734Risinger, Fred O.; Freeman, Pierre A.; Rubinstein, Marcelo; Low, Malcolm J.; Grandy, David K.; Lack of operant ethanol self-administration in dopamine D2 receptor knockout mice; Springer; Psychopharmacology; 152; 3; 10-2000; 343-3500033-31581432-2072CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/doi/10.1007/s002130000548info:eu-repo/semantics/altIdentifier/url/https://link.springer.com/article/10.1007%2Fs002130000548info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2024-05-08T14:10:55Zoai:ri.conicet.gov.ar:11336/71734instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982024-05-08 14:10:55.877CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Lack of operant ethanol self-administration in dopamine D2 receptor knockout mice
title Lack of operant ethanol self-administration in dopamine D2 receptor knockout mice
spellingShingle Lack of operant ethanol self-administration in dopamine D2 receptor knockout mice
Risinger, Fred O.
Continuous Access
Dopamine D2 Receptor Knockout Mice
Ethanol Self-Administration
Reinforcement
https://purl.org/becyt/ford/3.1
https://purl.org/becyt/ford/3
title_short Lack of operant ethanol self-administration in dopamine D2 receptor knockout mice
title_full Lack of operant ethanol self-administration in dopamine D2 receptor knockout mice
title_fullStr Lack of operant ethanol self-administration in dopamine D2 receptor knockout mice
title_full_unstemmed Lack of operant ethanol self-administration in dopamine D2 receptor knockout mice
title_sort Lack of operant ethanol self-administration in dopamine D2 receptor knockout mice
dc.creator.none.fl_str_mv Risinger, Fred O.
Freeman, Pierre A.
Rubinstein, Marcelo
Low, Malcolm J.
Grandy, David K.
author Risinger, Fred O.
author_facet Risinger, Fred O.
Freeman, Pierre A.
Rubinstein, Marcelo
Low, Malcolm J.
Grandy, David K.
author_role author
author2 Freeman, Pierre A.
Rubinstein, Marcelo
Low, Malcolm J.
Grandy, David K.
author2_role author
author
author
author
dc.subject.none.fl_str_mv Continuous Access
Dopamine D2 Receptor Knockout Mice
Ethanol Self-Administration
Reinforcement
https://purl.org/becyt/ford/3.1
https://purl.org/becyt/ford/3
topic Continuous Access
Dopamine D2 Receptor Knockout Mice
Ethanol Self-Administration
Reinforcement
https://purl.org/becyt/ford/3.1
https://purl.org/becyt/ford/3
description Rationale: Dopamine D2 receptors are postulated to play an important role in modulating the reinforcing effects of abused drugs including ethanol. Objectives: This experiment examined operant ethanol self-administration in dopamine D2 receptor knockout (KO) mice and wild-type (WT) mice using a continuous access procedure. Methods: Adult male KO and WT mice were trained in 30-min sessions to perform a lever press response for access to 10% v/v ethanol. After training, the mice were placed in test chambers on a continuous (23 h/day) basis with access to food (one lever press, i.e., FR1), 10% v/v ethanol (four lever presses, i.e., FR4), and water from a sipper tube (phase 1). After 30 consecutive sessions, response patterns were determined for 0, 5, 10, 20 and 30% v/v ethanol (phase 2). Saccharin (0.2% w/v) was subsequently added to the ethanol mixture and responding was examined for 0, 5, 10 and 20% ethanol (phase 3). Results: During phase 1, WT mice displayed higher ethanol-lever responding compared to KO mice. Food lever responding and water intake was the same in both genotypes. During phase 2, WT mice displayed concentration-dependent ethanol lever responding, whereas KO mice responded at low rates regardless of ethanol concentration. WT mice also responded more for food compared to KO mice. Each genotype showed similar water intakes except at the 20% ethanol concentration, where WT mice had lower intakes. During phase 3, WT mice continued to show higher responding for all concentrations including saccharin alone. WT mice also continued to respond more for food compared to KO mice, but drank less water. In each phase, WT mice displayed episodic (bout) responding on the ethanol lever. KO mice did not respond for ethanol in bouts. Conclusions: Reduced responding in the KO mice for several reinforcers including ethanol indicates a more general role for dopamine D2 receptors in motivated responding rather than a specific role in ethanol reinforcement.
publishDate 2000
dc.date.none.fl_str_mv 2000-10
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/71734
Risinger, Fred O.; Freeman, Pierre A.; Rubinstein, Marcelo; Low, Malcolm J.; Grandy, David K.; Lack of operant ethanol self-administration in dopamine D2 receptor knockout mice; Springer; Psychopharmacology; 152; 3; 10-2000; 343-350
0033-3158
1432-2072
CONICET Digital
CONICET
url http://hdl.handle.net/11336/71734
identifier_str_mv Risinger, Fred O.; Freeman, Pierre A.; Rubinstein, Marcelo; Low, Malcolm J.; Grandy, David K.; Lack of operant ethanol self-administration in dopamine D2 receptor knockout mice; Springer; Psychopharmacology; 152; 3; 10-2000; 343-350
0033-3158
1432-2072
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/doi/10.1007/s002130000548
info:eu-repo/semantics/altIdentifier/url/https://link.springer.com/article/10.1007%2Fs002130000548
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
dc.publisher.none.fl_str_mv Springer
publisher.none.fl_str_mv Springer
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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score 15,811543

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