Altered development, oxidative stress and DNA damage in Leptodactylus chaquensis (Anura: Leptodactylidae) larvae exposed to poultry litter

Poultry litter (PL), which is usually used as organic fertilizer, is a source of nutrients, metals, veterinary pharmaceuticals and bacterial pathogens, which, through runoff, may end up in the nearest aquatic ecosystems. In this study, Leptodactylus chaquensis at different development stages (eggs,...

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Authors: Curi, Lucila Marilén, Peltzer, Paola, Martinuzzi, Candela Soledad, Attademo, Andres Maximiliano, Seib, S., Simoniello, Maria Fernanda, Lajmanovich, Rafael Carlos
Format: article
Status:Published version
Publication Date:2017
Country:Argentina
Institution:Consejo Nacional de Investigaciones Científicas y Técnicas
Repository:CONICET Digital (CONICET)
Language:English
OAI Identifier:oai:ri.conicet.gov.ar:11336/42276
Online Access:http://hdl.handle.net/11336/42276
Access Level:Open access
Keyword:Poultry Production
Biological Endpoints
Anuran Tadpoles
Organic Fertilizer
https://purl.org/becyt/ford/1.6
https://purl.org/becyt/ford/1
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network_name_str Argentina
repository_id_str
dc.title.none.fl_str_mv Altered development, oxidative stress and DNA damage in Leptodactylus chaquensis (Anura: Leptodactylidae) larvae exposed to poultry litter
title Altered development, oxidative stress and DNA damage in Leptodactylus chaquensis (Anura: Leptodactylidae) larvae exposed to poultry litter
spellingShingle Altered development, oxidative stress and DNA damage in Leptodactylus chaquensis (Anura: Leptodactylidae) larvae exposed to poultry litter
Curi, Lucila Marilén
Poultry Production
Biological Endpoints
Anuran Tadpoles
Organic Fertilizer
https://purl.org/becyt/ford/1.6
https://purl.org/becyt/ford/1
title_short Altered development, oxidative stress and DNA damage in Leptodactylus chaquensis (Anura: Leptodactylidae) larvae exposed to poultry litter
title_full Altered development, oxidative stress and DNA damage in Leptodactylus chaquensis (Anura: Leptodactylidae) larvae exposed to poultry litter
title_fullStr Altered development, oxidative stress and DNA damage in Leptodactylus chaquensis (Anura: Leptodactylidae) larvae exposed to poultry litter
title_full_unstemmed Altered development, oxidative stress and DNA damage in Leptodactylus chaquensis (Anura: Leptodactylidae) larvae exposed to poultry litter
title_sort Altered development, oxidative stress and DNA damage in Leptodactylus chaquensis (Anura: Leptodactylidae) larvae exposed to poultry litter
dc.creator.none.fl_str_mv Curi, Lucila Marilén
Peltzer, Paola
Martinuzzi, Candela Soledad
Attademo, Andres Maximiliano
Seib, S.
Simoniello, Maria Fernanda
Lajmanovich, Rafael Carlos
author Curi, Lucila Marilén
author_facet Curi, Lucila Marilén
Peltzer, Paola
Martinuzzi, Candela Soledad
Attademo, Andres Maximiliano
Seib, S.
Simoniello, Maria Fernanda
Lajmanovich, Rafael Carlos
author_role author
author2 Peltzer, Paola
Martinuzzi, Candela Soledad
Attademo, Andres Maximiliano
Seib, S.
Simoniello, Maria Fernanda
Lajmanovich, Rafael Carlos
author2_role author
author
author
author
author
author
dc.subject.none.fl_str_mv Poultry Production
Biological Endpoints
Anuran Tadpoles
Organic Fertilizer
https://purl.org/becyt/ford/1.6
https://purl.org/becyt/ford/1
topic Poultry Production
Biological Endpoints
Anuran Tadpoles
Organic Fertilizer
https://purl.org/becyt/ford/1.6
https://purl.org/becyt/ford/1
description Poultry litter (PL), which is usually used as organic fertilizer, is a source of nutrients, metals, veterinary pharmaceuticals and bacterial pathogens, which, through runoff, may end up in the nearest aquatic ecosystems. In this study, Leptodactylus chaquensis at different development stages (eggs, larval stages 28 and 31 here referred to as stages I, II and III respectively) were exposed to PL test sediments as follows: 6.25% (T1), 12.5% (T2); 25% (T3); 50% (T4); 75% (T5); 100% PL (T6) and to dechlorinated water as control. Larval survival, development endpoints (growth rate -GR-, development rate -DR-, abnormalities), antioxidant enzyme activities (Catalase -CAT- and Glutathione-S-Transferase -GST-), and genotoxic effect (DNA damage index by the Comet assay) were analyzed at different times. In stage I, no egg eclosion was observed in treatments T3-T6, and 50% of embryo mortality was recorded after 24 h of exposure to T2. In stages II and III, mortality in treatments T3-T6 reached 100% between 24 and 48 h. In the three development stages evaluated, the DR and GR were higher in controls than in PL treatments (T1, T2), except for those T1-treated larvae of stage II. Larvae of stage I showed five types of morphological abnormalities, being diamond body shape and lateral displacement of the intestine the most prevalent in T1, whereas larvae of stages II and III presented lower prevalence of abnormalities. In stage I, CAT activity was similar to that of control (p>0.05), whereas it was higher in T1- and T2- treated larvae of stages II and III than controls (p<0.05). In stages I and III, GST activity was similar to that of controls (p>0.05), whereas it was inhibited in T1-treated larvae of stage II (p<0.05). T1- and T2-treated larvae of stages II and III caused higher DNA damage respect to controls (p<0.05), varying from medium to severe damage (comet types II, III and IV). These results showed that PL treatments altered development and growth and induced oxidative stress and DNA damage, resulting ecotoxic for L. chaquensis larvae.
publishDate 2017
dc.date.none.fl_str_mv 2017-09
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/42276
Curi, Lucila Marilén; Peltzer, Paola; Martinuzzi, Candela Soledad; Attademo, Andres Maximiliano; Seib, S.; et al.; Altered development, oxidative stress and DNA damage in Leptodactylus chaquensis (Anura: Leptodactylidae) larvae exposed to poultry litter; Academic Press Inc Elsevier Science; Ecotoxicology and Environmental Safety; 143; 9-2017; 62-71
0147-6513
CONICET Digital
CONICET
url http://hdl.handle.net/11336/42276
identifier_str_mv Curi, Lucila Marilén; Peltzer, Paola; Martinuzzi, Candela Soledad; Attademo, Andres Maximiliano; Seib, S.; et al.; Altered development, oxidative stress and DNA damage in Leptodactylus chaquensis (Anura: Leptodactylidae) larvae exposed to poultry litter; Academic Press Inc Elsevier Science; Ecotoxicology and Environmental Safety; 143; 9-2017; 62-71
0147-6513
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/url/https://www.sciencedirect.com/science/article/pii/S0147651317302804
info:eu-repo/semantics/altIdentifier/doi/10.1016/j.ecoenv.2017.05.007
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-nd/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-nd/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
application/pdf
application/pdf
application/pdf
dc.publisher.none.fl_str_mv Academic Press Inc Elsevier Science
publisher.none.fl_str_mv Academic Press Inc Elsevier Science
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
_version_ 1799194876537995264
spelling Altered development, oxidative stress and DNA damage in Leptodactylus chaquensis (Anura: Leptodactylidae) larvae exposed to poultry litterCuri, Lucila MarilénPeltzer, PaolaMartinuzzi, Candela SoledadAttademo, Andres MaximilianoSeib, S.Simoniello, Maria FernandaLajmanovich, Rafael CarlosPoultry ProductionBiological EndpointsAnuran TadpolesOrganic Fertilizerhttps://purl.org/becyt/ford/1.6https://purl.org/becyt/ford/1Poultry litter (PL), which is usually used as organic fertilizer, is a source of nutrients, metals, veterinary pharmaceuticals and bacterial pathogens, which, through runoff, may end up in the nearest aquatic ecosystems. In this study, Leptodactylus chaquensis at different development stages (eggs, larval stages 28 and 31 here referred to as stages I, II and III respectively) were exposed to PL test sediments as follows: 6.25% (T1), 12.5% (T2); 25% (T3); 50% (T4); 75% (T5); 100% PL (T6) and to dechlorinated water as control. Larval survival, development endpoints (growth rate -GR-, development rate -DR-, abnormalities), antioxidant enzyme activities (Catalase -CAT- and Glutathione-S-Transferase -GST-), and genotoxic effect (DNA damage index by the Comet assay) were analyzed at different times. In stage I, no egg eclosion was observed in treatments T3-T6, and 50% of embryo mortality was recorded after 24 h of exposure to T2. In stages II and III, mortality in treatments T3-T6 reached 100% between 24 and 48 h. In the three development stages evaluated, the DR and GR were higher in controls than in PL treatments (T1, T2), except for those T1-treated larvae of stage II. Larvae of stage I showed five types of morphological abnormalities, being diamond body shape and lateral displacement of the intestine the most prevalent in T1, whereas larvae of stages II and III presented lower prevalence of abnormalities. In stage I, CAT activity was similar to that of control (p>0.05), whereas it was higher in T1- and T2- treated larvae of stages II and III than controls (p<0.05). In stages I and III, GST activity was similar to that of controls (p>0.05), whereas it was inhibited in T1-treated larvae of stage II (p<0.05). T1- and T2-treated larvae of stages II and III caused higher DNA damage respect to controls (p<0.05), varying from medium to severe damage (comet types II, III and IV). These results showed that PL treatments altered development and growth and induced oxidative stress and DNA damage, resulting ecotoxic for L. chaquensis larvae.Fil: Curi, Lucila Marilén. Universidad Nacional del Litoral. Facultad de Bioquímica y Ciencias Biológicas. Laboratorio de Saneamiento Ambiental. Cátedra de Ecotoxicología; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaFil: Peltzer, Paola. Universidad Nacional del Litoral. Facultad de Bioquímica y Ciencias Biológicas. Laboratorio de Saneamiento Ambiental. Cátedra de Ecotoxicología; ArgentinaFil: Martinuzzi, Candela Soledad. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Universidad Nacional del Litoral. Facultad de Bioquímica y Ciencias Biológicas. Laboratorio de Saneamiento Ambiental. Cátedra de Ecotoxicología; ArgentinaFil: Attademo, Andres Maximiliano. Universidad Nacional del Litoral. Facultad de Bioquímica y Ciencias Biológicas. Laboratorio de Saneamiento Ambiental. Cátedra de Ecotoxicología; ArgentinaFil: Seib, S.. Universidad Nacional del Litoral. Facultad de Bioquímica y Ciencias Biológicas. Laboratorio de Saneamiento Ambiental. Cátedra de Ecotoxicología; ArgentinaFil: Simoniello, Maria Fernanda. Universidad Nacional del Litoral. Facultad de Bioquímica y Ciencias Biológicas. Laboratorio de Saneamiento Ambiental. Cátedra de Ecotoxicología; ArgentinaFil: Lajmanovich, Rafael Carlos. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Universidad Nacional del Litoral. Facultad de Bioquímica y Ciencias Biológicas. Laboratorio de Saneamiento Ambiental. Cátedra de Ecotoxicología; ArgentinaAcademic Press Inc Elsevier Science2017-09info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfapplication/pdfapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/42276Curi, Lucila Marilén; Peltzer, Paola; Martinuzzi, Candela Soledad; Attademo, Andres Maximiliano; Seib, S.; et al.; Altered development, oxidative stress and DNA damage in Leptodactylus chaquensis (Anura: Leptodactylidae) larvae exposed to poultry litter; Academic Press Inc Elsevier Science; Ecotoxicology and Environmental Safety; 143; 9-2017; 62-710147-6513CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/url/https://www.sciencedirect.com/science/article/pii/S0147651317302804info:eu-repo/semantics/altIdentifier/doi/10.1016/j.ecoenv.2017.05.007info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-nd/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2024-05-08T13:38:28Zoai:ri.conicet.gov.ar:11336/42276instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982024-05-08 13:38:29.06CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
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