rptA, a novel gene from Ensifer (Sinorhizobium) meliloti involved in conjugal transfer

We approached the identification of Ensifer (Sinorhizobium) meliloti conjugal functions by random Tn5-B13 mutagenesis of the pSmeLPU88a plasmid of E. meliloti strain LPU88 and the subsequent selection of those mutants that had lost the ability to mobilize the small plasmid pSmeLPU88b. The Tn5-B13-in...

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Authors: Pistorio, Mariano, Torres Tejerizo, Gonzalo Arturo, Del Papa, María Florencia, Giusti, María de los Ángeles, Lozano, Mauricio Javier, Lagares, Antonio
Format: article
Status:Published version
Publication Date:2013
Country:Argentina
Institution:Universidad Nacional de La Plata
Repository:SEDICI (UNLP)
Language:English
OAI Identifier:oai:sedici.unlp.edu.ar:10915/85390
Online Access:http://sedici.unlp.edu.ar/handle/10915/85390
Access Level:Open access
Keyword:Ciencias Exactas
Conjugation
Ensifer meliloti
Plasmid
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spelling rptA, a novel gene from Ensifer (Sinorhizobium) meliloti involved in conjugal transferPistorio, MarianoTorres Tejerizo, Gonzalo ArturoDel Papa, María FlorenciaGiusti, María de los ÁngelesLozano, Mauricio JavierLagares, AntonioCiencias ExactasConjugationEnsifer melilotiPlasmidWe approached the identification of Ensifer (Sinorhizobium) meliloti conjugal functions by random Tn5-B13 mutagenesis of the pSmeLPU88a plasmid of E. meliloti strain LPU88 and the subsequent selection of those mutants that had lost the ability to mobilize the small plasmid pSmeLPU88b. The Tn5-B13-insertion site of one of the mutants was cloned as an EcoRI-restricted DNA fragment that after subsequent isolation and sequencing demonstrated that a small open reading frame of 522 bp (designated rptA, for rhizobium plasmid transfer A) had been disrupted. The predicted gene product encoded by the rptA sequence shows a significant similarity to two hypothetical proteins of the plasmid pSmed03 of Ensifer medicae WSM419 and other rhizobia plasmids. No significant similarity was found to any protein sequence of known function registered in the databases. Although the rptA gene was required for pSmeLPU88b-plasmid mobilization in the strain 2011 background, it was not required in the original strain LPU88 background.Instituto de Biotecnologia y Biologia Molecular2013info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionArticulohttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdf22-30http://sedici.unlp.edu.ar/handle/10915/85390enginfo:eu-repo/semantics/altIdentifier/issn/0378-1097info:eu-repo/semantics/altIdentifier/doi/10.1111/1574-6968.12177info:eu-repo/semantics/openAccesshttp://creativecommons.org/licenses/by-nc-sa/4.0/Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International (CC BY-NC-SA 4.0)reponame:SEDICI (UNLP)instname:Universidad Nacional de La Platainstacron:UNLP2024-05-08T12:54:31Zoai:sedici.unlp.edu.ar:10915/85390Institucionalhttp://sedici.unlp.edu.ar/Universidad públicaNo correspondehttp://sedici.unlp.edu.ar/oai/snrdalira@sedici.unlp.edu.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:13292024-05-08 12:54:32.034SEDICI (UNLP) - Universidad Nacional de La Platafalse
dc.title.none.fl_str_mv rptA, a novel gene from Ensifer (Sinorhizobium) meliloti involved in conjugal transfer
title rptA, a novel gene from Ensifer (Sinorhizobium) meliloti involved in conjugal transfer
spellingShingle rptA, a novel gene from Ensifer (Sinorhizobium) meliloti involved in conjugal transfer
Pistorio, Mariano
Ciencias Exactas
Conjugation
Ensifer meliloti
Plasmid
title_short rptA, a novel gene from Ensifer (Sinorhizobium) meliloti involved in conjugal transfer
title_full rptA, a novel gene from Ensifer (Sinorhizobium) meliloti involved in conjugal transfer
title_fullStr rptA, a novel gene from Ensifer (Sinorhizobium) meliloti involved in conjugal transfer
title_full_unstemmed rptA, a novel gene from Ensifer (Sinorhizobium) meliloti involved in conjugal transfer
title_sort rptA, a novel gene from Ensifer (Sinorhizobium) meliloti involved in conjugal transfer
dc.creator.none.fl_str_mv Pistorio, Mariano
Torres Tejerizo, Gonzalo Arturo
Del Papa, María Florencia
Giusti, María de los Ángeles
Lozano, Mauricio Javier
Lagares, Antonio
author Pistorio, Mariano
author_facet Pistorio, Mariano
Torres Tejerizo, Gonzalo Arturo
Del Papa, María Florencia
Giusti, María de los Ángeles
Lozano, Mauricio Javier
Lagares, Antonio
author_role author
author2 Torres Tejerizo, Gonzalo Arturo
Del Papa, María Florencia
Giusti, María de los Ángeles
Lozano, Mauricio Javier
Lagares, Antonio
author2_role author
author
author
author
author
dc.subject.none.fl_str_mv Ciencias Exactas
Conjugation
Ensifer meliloti
Plasmid
topic Ciencias Exactas
Conjugation
Ensifer meliloti
Plasmid
description We approached the identification of Ensifer (Sinorhizobium) meliloti conjugal functions by random Tn5-B13 mutagenesis of the pSmeLPU88a plasmid of E. meliloti strain LPU88 and the subsequent selection of those mutants that had lost the ability to mobilize the small plasmid pSmeLPU88b. The Tn5-B13-insertion site of one of the mutants was cloned as an EcoRI-restricted DNA fragment that after subsequent isolation and sequencing demonstrated that a small open reading frame of 522 bp (designated rptA, for rhizobium plasmid transfer A) had been disrupted. The predicted gene product encoded by the rptA sequence shows a significant similarity to two hypothetical proteins of the plasmid pSmed03 of Ensifer medicae WSM419 and other rhizobia plasmids. No significant similarity was found to any protein sequence of known function registered in the databases. Although the rptA gene was required for pSmeLPU88b-plasmid mobilization in the strain 2011 background, it was not required in the original strain LPU88 background.
publishDate 2013
dc.date.none.fl_str_mv 2013
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
Articulo
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://sedici.unlp.edu.ar/handle/10915/85390
url http://sedici.unlp.edu.ar/handle/10915/85390
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/issn/0378-1097
info:eu-repo/semantics/altIdentifier/doi/10.1111/1574-6968.12177
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
http://creativecommons.org/licenses/by-nc-sa/4.0/
Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International (CC BY-NC-SA 4.0)
eu_rights_str_mv openAccess
rights_invalid_str_mv http://creativecommons.org/licenses/by-nc-sa/4.0/
Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International (CC BY-NC-SA 4.0)
dc.format.none.fl_str_mv application/pdf
22-30
dc.source.none.fl_str_mv reponame:SEDICI (UNLP)
instname:Universidad Nacional de La Plata
instacron:UNLP
instname_str Universidad Nacional de La Plata
instacron_str UNLP
institution UNLP
reponame_str SEDICI (UNLP)
collection SEDICI (UNLP)
repository.name.fl_str_mv SEDICI (UNLP) - Universidad Nacional de La Plata
repository.mail.fl_str_mv alira@sedici.unlp.edu.ar
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